An Osmium(VI) Nitride Triggers Mitochondria-induced Oncosis and Apoptosis

2022 ◽  
Author(s):  
Meng Ye ◽  
Wan-Qiong Huang ◽  
Zi-Xin Li ◽  
Chuan-Xian Wang ◽  
Tao Liu ◽  
...  
Keyword(s):  
Cell Death ◽  
Liver Cancer ◽  
Anticancer Activity ◽  
Cancer Cell ◽  
Mitochondrial Damage ◽  
Tetradentate Ligand ◽  
Liver Cancer Cell ◽  
Cancer Cell Death

We report a new osmium(VI) nitrido complex bearing a nonplanar tetradentate ligand with potent anticancer activity. It causes mitochondrial damage, which induces liver cancer cell death via oncosis and apoptosis....

scholarly journals A small library of chalcones induce liver cancer cell death through Akt phosphorylation inhibition

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Irem Durmaz Sahin ◽  
Michael S. Christodoulou ◽  
Ece Akhan Guzelcan ◽  
Altay Koyas ◽  
Cigdem Karaca ◽  
...  
Keyword(s):  
Cell Death ◽  
Liver Cancer ◽  
Cancer Cell ◽  
Liver Cancer Cell ◽  
Akt Phosphorylation ◽  
Cancer Cell Death

scholarly journals Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway through inhibiting cytosolic ferritin and inducing chromotin autophagy

2020 ◽  
Author(s):  
Qingyuan Feng ◽  
Xuzhao Bian ◽  
Xuan Liu ◽  
Ying Wang ◽  
Huiting Zhou ◽  
...  
Keyword(s):  
Cell Death ◽  
Western Blot ◽  
Cancer Cell ◽  
Malignant Tumors ◽  
Mitochondrial Damage ◽  
Arginine Deiminase ◽  
Immunofluorescent Staining ◽  
Apoptosis Pathway ◽  
P53 Expression ◽  
Cancer Cell Death

Abstract Background：Based on its low toxicity, arginine starvation therapy has the potential to treat those malignant tumors that can’t be treated by surgery. Arginine deiminase (ADI) gene is indicated to be an ideal cancer-suppressor gene. ADI expressed in cytosol displays higher oncolytic efficiency than ADI-PEG20 (Pegylated Arginine Deiminase by PEG 20,000). However, it is still unknown whether cytosolic ADI has the same function mechanism as ADI-PEG20 or other underlying mechanisms in cells.Methods: The interaction of ADI and other protein factors was screened by yeast hybrid, and verified by co-immunoprecipitation and immunofluorescent staining. The effect of ADI inhibiting ferritin light-chain domain (FTL) on mitochondrial damage was evaluated by site-directed mutation and flow cytometry. The apoptosis pathway of mitochondria control was analyzed by Western Blot and real-time PCR experiments. The effect of p53 expression on cancer cell death was assessed by siTP53 transfection. The chromatin autophagy was explored by immunofluorescent staining and Western Blot.Results: ADI expressed in cytosol inhibited the activity of cytosolic ferritin through interacting with FTL. The inactive mutant of ADI still induced the apoptosis in certain cell lines of ASS- through mitochondrial damage. Arginine starvation also induced the increased expression of p53 and p53AIP1, which aggravate cellular mitochondrial damage. Chromatin autophagy appeared at the later stage of arginine starvation. DNA damage came along with the whole process of arginine starvation. Histone 3 (H3) was found in autophagosomes, which implied that cancer cells try to utilize the arginine in histones to survive during arginine starvation. Conclusions: Mitochondrial damage is the major mechanism of cell death induced by cytosolic ADI. Chromatophagy accumulations not only drive cancer cell to utilize histone arginine but also speed up cancer cell death at the later time point of arginine starvation.

scholarly journals Anticancer (liver cancer cell lines) and antioxidant activity of Cassia auriculata flower extract from acetone and methanol solvents

2018 ◽  
Vol 8 (6-s) ◽  
pp. 274-278 ◽  
Author(s):  
K Padmalochana
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Liver Cancer ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Acetone Extract ◽  
Liver Cancer Cell ◽  
Cassia Auriculata

This research paper was communicated to elucidate the antioxidant and anticancer activities of acetone and methanol extract of Cassia auriculata flower. Antioxidant activity was assessed against DPPH and hydroxyl free radical. The anticancer activity was examined by inhibition on liver cancer cell lines. The highest antioxidant activity was found for methanol extract (IC50: 46.28 μg/ml) than acetone extract (IC50: 52.60 μg/ml) on DPPH free radical. The IC50 values for methanol and acetone extract was found to be 47.05 and 56.01±1.85 µg/ml, respectively on hydroxyl radical. The highest anticancer activity was found for methanol extract to be 36.10±2.46 µg/ml. Among these extract methanol extract have high antioxidant and anticancer activity. Keywords: Antioxidant, anticancer, Plant

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