A novel ratiometric MALDI-MS quantitation strategy for alkaline phosphatase activity with a homogeneous reaction and a tunable dynamic range

2021 ◽  
Author(s):  
Rong-Na Ma ◽  
Min Zhang ◽  
Chao-Long Hu ◽  
Hui-Jing Pan ◽  
Lei Si ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Dynamic Range ◽  
Maldi Ms ◽  
Enzymatic Cleavage ◽  
Homogeneous Reaction ◽  
Signal Output

A unique ratiometric MALDI-MS strategy based on a homogeneous enzymatic cleavage triggered double-signal output was designed for the convenient and reliable quantitation of alkaline phosphatase with a tunable dynamic range.

LEUCOCYTE METABOLISM IN PREGNANCY

1960 ◽  
Vol XXXV (IV) ◽  
pp. 575-584 ◽  
Author(s):  
C. Borel ◽  
J. Frei ◽  
A. Vannotti
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Consumption ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lactate Production ◽  
Glucose Consumption ◽  
In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

Alkaline phosphatase activity and the development of rat sponge-induced granulation tissue

1972 ◽  
Vol 83 (1) ◽  
pp. 60-69 ◽  
Author(s):  
Mário Roberto Vizioli ◽  
Lourenço Bozzo ◽  
Luiz Valdrighi
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Granulation Tissue
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