Dipolar Coupling-Based Electron Paramagnetic Resonance Method for Protease Enzymatic Characterization and Inhibitor Screening

2021 ◽  
Author(s):  
Lu Yu ◽  
Aokun Liu ◽  
Bingbo Zhang ◽  
Jian Kuang ◽  
Xiaoqi Guo ◽  
...  

Herein, we report an EPR-based method for protease enzymatic characterization and inhibitor screening. This method utilizes dual paramagnetically-labeled probes consisting of a nitroxide spin probe and a Gd3+ ion flanking...

2010 ◽  
Vol 5 (1) ◽  
pp. 97-101
Author(s):  
Sergey P. Bardakhanov ◽  
Aleksandr M. Volodin ◽  
Konstantin V. Zobov ◽  
Roman M. Kenzhin ◽  
Vladimir I. Lysenko

The paper is devoted to experimental study of alumina and other nanopowders by electron paramagnetic resonance method. The experiments have showed the surface donor centers for ZrO2 + MgO (9 % mol.) and Al2O3 samples. The influence of Fe(3+) dirt was determined in glacial Al2O3 liquid melt, but Al2O3 nanopowder was free from this impurity. The Cr(5+) dirt were found in ZrO2 + MgO (9 % mol.) nanopowder sample.


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