Structure-Selective Differentiation of Deletion Mutation in Circulating Tumor DNA by Dual Probe-Based Isothermal Amplification

2021 ◽  
Author(s):  
Yusong Wang ◽  
Say Li Kong ◽  
Xiaodi Su

A rapid dual probe-based fluorimetric assay was developed to detect deletion mutation in circulating tumor DNA, through structure-selective isothermal amplification and pattern recognition. This method can detect both homozygous and...

RSC Advances ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 1476-1483 ◽  
Author(s):  
Yusong Wang ◽  
Say Li Kong ◽  
Xiao Di Su

A centrifugation-assisted dual signal amplified visual detection of ctDNA SNP (∼150 nt, 1% clinic sensitivity) within 2 hours has been reported.


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