Dynamic visualization of mRNA splicing variants with a transactivating reporter

2021 ◽  
Author(s):  
Beilei Zhang ◽  
Wenjie Shu ◽  
Zhe Ma ◽  
Miao Li ◽  
Fu Wang
Keyword(s):  
Noninvasive Monitoring ◽  
Mrna Splicing ◽  
Dynamic Visualization ◽  
Intron Sequence ◽  
Dynamic Changes ◽  
Splicing Variants

The dynamic changes for intron sequence with their loss and gain are poorly detected due to the limited methods for noninvasive monitoring pre-mRNA splicing process. Here we described the design...

scholarly journals Correction: Dynamic visualization of mRNA splicing variants with a transactivating reporter

2021 ◽  
Vol 57 (75) ◽  
pp. 9626-9626
Author(s):  
Si Chen ◽  
Wenjie Shu ◽  
Haifeng Zheng ◽  
Zhe Ma ◽  
Miao Li ◽  
...  
Keyword(s):  
Mrna Splicing ◽  
Dynamic Visualization ◽  
Splicing Variants

Correction for ‘Dynamic visualization of mRNA splicing variants with a transactivating reporter’ by Si Chen et al., Chem. Commun., 2021, DOI: 10.1039/d1cc02439f.

scholarly journals NEIL1 mRNA Splicing Variants are Expressed in Normal Mouse Organs

2012 ◽  
Vol 53 (2) ◽  
pp. 234-241 ◽  
Author(s):  
Ryohei YAMAMOTO ◽  
Mizuki YAMAMOTO ◽  
Hiroyuki KUSAKA ◽  
Hideaki MASATSUGU ◽  
Satoshi MATSUYAMA ◽  
...  
Keyword(s):  
Normal Mouse ◽  
Mrna Splicing ◽  
Splicing Variants

scholarly journals SpliceRCA: in Situ Single-Cell Analysis of mRNA Splicing Variants

2018 ◽  
Vol 4 (6) ◽  
pp. 680-687 ◽  
Author(s):  
Xiaojun Ren ◽  
Ruijie Deng ◽  
Kaixiang Zhang ◽  
Yupeng Sun ◽  
Xucong Teng ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Mrna Splicing ◽  
Cell Analysis ◽  
Splicing Variants

scholarly journals Real-time detection of mRNA splicing variants with specifically designed reverse-transcription loop-mediated isothermal amplification

RSC Advances ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 6271-6276
Author(s):  
Fengxia Su ◽  
Guanhao Wang ◽  
Jianing Ji ◽  
Pengbo Zhang ◽  
Fangfang Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Mrna Splicing ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Splicing Variant ◽  
Splicing Variants ◽  
Real Time Detection

A novel splicing variant assay is developed based on specifically designed reverse-transcription (RT) loop-mediated isothermal amplification.

scholarly journals Validation of predicted mRNA splicing mutations using high-throughput transcriptome data

F1000Research ◽  
2014 ◽  
Vol 3 ◽  
pp. 8
Author(s):  
Coby Viner ◽  
Stephanie N. Dorman ◽  
Ben C. Shirley ◽  
Peter K. Rogan
Keyword(s):  
Exon Skipping ◽  
Mrna Splicing ◽  
Cryptic Splice Site ◽  
Sequencing Data ◽  
Manual Inspection ◽  
Large Numbers ◽  
Splicing Variants ◽  
Complete Genomes ◽  
Site Use ◽  
Splicing Patterns

Interpretation of variants present in complete genomes or exomes reveals numerous sequence changes, only a fraction of which are likely to be pathogenic. Mutations have been traditionally inferred from allele frequencies and inheritance patterns in such data. Variants predicted to alter mRNA splicing can be validated by manual inspection of transcriptome sequencing data, however this approach is intractable for large datasets. These abnormal mRNA splicing patterns are characterized by reads demonstrating either exon skipping, cryptic splice site use, and high levels of intron inclusion, or combinations of these properties. We present, Veridical, anin silicomethod for the automatic validation of DNA sequencing variants that alter mRNA splicing. Veridical performs statistically valid comparisons of the normalized read counts of abnormal RNA species in mutant versus non-mutant tissues. This leverages large numbers of control samples to corroborate the consequences of predicted splicing variants in complete genomes and exomes.

scholarly journals Corepressors: Custom Tailoring and Alterations While you Wait

2005 ◽  
Vol 3 (1) ◽  
pp. nrs.03003 ◽  
Author(s):  
Michael Goodson ◽  
Brian A. Jonas ◽  
Martin A. Privalsky
Keyword(s):  
Transcription Factor ◽  
Transcriptional Repression ◽  
Developmental Stages ◽  
Thyroid Hormone Receptor ◽  
Protein Complexes ◽  
Biological Properties ◽  
Mrna Splicing ◽  
Alternative Mrna Splicing ◽  
Splicing Variants ◽  
Protein Variants

A diverse cadre of metazoan transcription factors mediate repression by recruiting protein complexes containing the SMRT (silencing mediator of retinoid and thyroid hormone receptor) or N-CoR (nuclear receptor corepressor) corepressors. SMRT and N-CoR nucleate the assembly of still larger corepressor complexes that perform the specific molecular incantations necessary to confer transcriptional repression. Although SMRT and N-CoR are paralogs and possess similar molecular architectures and mechanistic strategies, they nonetheless exhibit distinct molecular and biological properties. It is now clear that the functions of both SMRT and N-CoR are further diversified through alternative mRNA splicing, yielding a series of corepressor protein variants that participate in distinctive transcription factor partnerships and display distinguishable repression properties. This review will discuss what is known about the structure and actions of SMRT, N-CoR, and their splicing variants, and how alternative splicing may allow the functions of these corepressors to be adapted and tailored to different cells and to different developmental stages.

scholarly journals Enhanced Risk Profiling of Implanted Defibrillator Shocks With Circulating SCN5A mRNA Splicing Variants

2014 ◽  
Vol 63 (21) ◽  
pp. 2261-2269 ◽  
Author(s):  
Ge Gao ◽  
Vikram Brahmanandam ◽  
Mihai Raicu ◽  
Lianzhi Gu ◽  
Li Zhou ◽  
...  
Keyword(s):  
Mrna Splicing ◽  
Risk Profiling ◽  
Splicing Variants

scholarly journals Expression and physiological role of CCN4/Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes

FEBS Journal ◽  
2007 ◽  
Vol 274 (7) ◽  
pp. 1655-1665 ◽  
Author(s):  
Takeshi Yanagita ◽  
Satoshi Kubota ◽  
Harumi Kawaki ◽  
Kazumi Kawata ◽  
Seiji Kondo ◽  
...  
Keyword(s):  
Physiological Role ◽  
Mrna Splicing ◽  
Secreted Protein ◽  
Splicing Variants

scholarly journals Identification of novel HNF1B mRNA splicing variants and their qualitative and semi-quantitative profile in selected healthy and tumour tissues

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jan Hojny ◽  
Michaela Bartu ◽  
Eva Krkavcova ◽  
Kristyna Nemejcova ◽  
Jan Sevcik ◽  
...  
Keyword(s):  
Mrna Splicing ◽  
Splicing Variants

scholarly journals DETERMINATION OF LEVEL EXPRESSION OF mRNA SPLICING VARIANTS FOR DR3 IN BLOOD CELLS IN INFECTIOUS MONONUCLEOSIS

2016 ◽  
Vol 18 (2) ◽  
pp. 139-150
Author(s):  
V. D. Cvetkova ◽  
N. A. Sakharnov ◽  
D. I. Knyazev ◽  
O. V. Utkin ◽  
N. V. Neumoina ◽  
...  
Keyword(s):  
Infectious Mononucleosis ◽  
Blood Cells ◽  
Mrna Splicing ◽  
Splicing Variants
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