scholarly journals A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

2021 ◽  
Author(s):  
Subha Bakthavatsalam ◽  
Kosuke Dodo ◽  
Mikiko Sodeoka
Keyword(s):  
Functional Groups ◽  
Biological Samples ◽  
Biological Systems ◽  
Raman Imaging ◽  
Silent Region

Alkyne functional groups have unique stretching frequency in the cell silent region. This review discusses the application of alkyne tags for Raman imaging in biological samples.

scholarly journals Polydiacetylene-based ultrastrong bioorthogonal Raman probes for targeted live-cell Raman imaging

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Sidan Tian ◽  
Haozheng Li ◽  
Zhong Li ◽  
Huajun Tang ◽  
Mingming Yin ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Raman Imaging ◽  
Live Cell ◽  
Water Soluble ◽  
Imaging Agents ◽  
Great Promise ◽  
High Quality ◽  
Topochemical Polymerization ◽  
Silent Region

AbstractLive-cell Raman imaging based on bioorthogonal Raman probes with distinct signals in the cellular Raman-silent region (1800–2800 cm−1) has attracted great interest in recent years. We report here a class of water-soluble and biocompatible polydiacetylenes with intrinsic ultrastrong alkyne Raman signals that locate in this region for organelle-targeting live-cell Raman imaging. Using a host-guest topochemical polymerization strategy, we have synthesized a water-soluble and functionalizable master polydiacetylene, namely poly(deca-4,6-diynedioic acid) (PDDA), which possesses significantly enhanced (up to ~104 fold) alkyne vibration compared to conventional alkyne Raman probes. In addition, PDDA can be used as a general platform for multi-functional ultrastrong Raman probes. We achieve high quality live-cell stimulated Raman scattering imaging on the basis of modified PDDA. The polydiacetylene-based Raman probes represent ultrastrong intrinsic Raman imaging agents in the Raman-silent region (without any Raman enhancer), and the flexible functionalization of this material holds great promise for its potential diverse applications.

Raman tags derived from dyes encapsulated inside carbon nanotubes for Raman imaging of biological samples

2014 ◽  
Vol 211 (12) ◽  
pp. 2790-2794 ◽  
Author(s):  
Nicolas Cottenye ◽  
Nathalie Y-Wa Tang ◽  
Etienne Gaufrès ◽  
Annie Leduc ◽  
Jean Barbeau ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Biological Samples ◽  
Raman Imaging

Facile synthesis of zwitterionic polymer-coated core–shell magnetic nanoparticles for highly specific capture of N-linked glycopeptides

Nanoscale ◽  
2015 ◽  
Vol 7 (7) ◽  
pp. 3100-3108 ◽  
Author(s):  
Yajing Chen ◽  
Zhichao Xiong ◽  
Lingyi Zhang ◽  
Jiaying Zhao ◽  
Quanqing Zhang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Biological Samples ◽  
Biological Systems ◽  
Core Shell ◽  
Facile Synthesis ◽  
Disease Biomarkers ◽  
Zwitterionic Polymer ◽  
Proteins And Peptides ◽  
Glycosylated Proteins

Highly selective and efficient capture of glycosylated proteins and peptides from complex biological samples is of profound significance for the discovery of disease biomarkers in biological systems.

scholarly journals Synthetic mimetics of protein secondary structure domains

2010 ◽  
Vol 368 (1914) ◽  
pp. 989-1008 ◽  
Author(s):  
Nathan T. Ross ◽  
William P. Katt ◽  
Andrew D. Hamilton
Keyword(s):  
Secondary Structure ◽  
Functional Groups ◽  
Biological Systems ◽  
Protein Secondary Structure ◽  
Protein Recognition ◽  
Structural Elements ◽  
Biological Functions ◽  
Major Focus ◽  
The Past ◽  
Secondary Structural Elements

Proteins modulate the majority of all biological functions and are primarily composed of highly organized secondary structural elements such as helices, turns and sheets. Many of these functions are affected by a small number of key protein–protein contacts, often involving one or more of these well-defined structural elements. Given the ubiquitous nature of these protein recognition domains, their mimicry by peptidic and non-peptidic scaffolds has become a major focus of contemporary research. This review examines several key advances in secondary structure mimicry over the past several years, particularly focusing upon scaffolds that show not only promising projection of functional groups, but also a proven effect in biological systems.

Subwavelength Raman imaging of biological samples using near-field spectroscopy

1999 ◽  
Author(s):  
Volker Deckert ◽  
Dieter Zeisel ◽  
Renato Zenobi ◽  
Tuan Vo-Dinh
Keyword(s):  
Biological Samples ◽  
Near Field ◽  
Raman Imaging ◽  
Field Spectroscopy

Metabolic remodeling of bacterial surfaces via tetrazine ligations

2015 ◽  
Vol 51 (51) ◽  
pp. 10330-10333 ◽  
Author(s):  
S. E. Pidgeon ◽  
M. M. Pires
Keyword(s):  
Functional Groups ◽  
Biological Systems ◽  
Metabolic Remodeling ◽  
Bacterial Surfaces

Bioorthogonal click ligations are extensively used for the introduction of functional groups in biological systems.

scholarly journals Physical and Methodological Perspectives on the Optical Properties of Biological Samples: A Review

Photonics ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 540
Author(s):  
George I. Lambrou ◽  
Anna Tagka ◽  
Athanasios Kotoulas ◽  
Argyro Chatziioannou ◽  
George K. Matsopoulos
Keyword(s):  
Optical Properties ◽  
Biological Samples ◽  
Biological Systems ◽  
Disease Diagnosis ◽  
High Energy ◽  
X Rays ◽  
Γ Radiation ◽  
Theoretical Concepts ◽  
Tissue Optical Properties ◽  
Depth Study

The optical properties of biological systems can be measured by imaging and microscopy methodologies. The use of X-rays, γ-radiation and electron microscopy provides information about the contents and functions of the systems. The need to develop imaging methods and analyses to measure these optical properties is increasing. On the other hand, biological samples are easily penetrated by a high-energy input, which has revolutionized the field of tissue optical properties and has now reached a point where light can be applied in the diagnosis and treatment of diseases. To this end, developing methodologies would allow the in-depth study of optical properties of tissues. In the present work, we review the literature focusing on optical properties of biological systems and tissues. We have reviewed the literature for related articles on biological samples’ optical properties. We have reported on the theoretical concepts and the applications of Monte Carlo simulations in the studies of optical properties of biological samples. Optical properties of biological samples are of paramount importance for the understanding of biological samples as well as for their applications in disease diagnosis and therapy.

scholarly journals In vivo generation of DNA sequence diversity for cellular barcoding

2014 ◽  
Author(s):  
Ian Peikon ◽  
Diana Gizatullina ◽  
Anthony Zador
Keyword(s):  
Dna Sequencing ◽  
Dna Sequence ◽  
Biological Samples ◽  
Biological Systems ◽  
Sequence Diversity ◽  
Bacterial Cells ◽  
Complete Understanding ◽  
Novel Method ◽  
Cellular Barcoding

Heterogeneity is a ubiquitous feature of biological systems. A complete understanding of such systems requires a method for uniquely identifying and tracking individual components and their interactions with each other. We have developed a novel method of uniquely tagging individual cells in vivo with a genetic 'barcode' that can be recovered by DNA sequencing. We demonstrate the feasibility of this technique in bacterial cells. This method should prove useful in tracking interactions of cells within a network, and/or heterogeneity within complex biological samples.

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