scholarly journals Segmental and site specific isotope labelling strategies for structural analysis of posttranslationally modified proteins

2021 ◽  
Author(s):  
Dominik P Vogl ◽  
Anne C. Conibear ◽  
Christian F.W. Becker
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Raman Spectroscopy ◽  
Structural Analysis ◽  
Posttranslational Modifications ◽  
Protein Structures ◽  
Spectroscopic Techniques ◽  
Site Specific ◽  
Isotope Labelling ◽  
Infrared And Raman Spectroscopy ◽  
Modified Proteins

Posttranslational modifications can alter protein structures, functions and localisation, and are important cellular regulatory and signalling mechanisms. Spectroscopic techniques such as nuclear magnetic resonance, infrared, and Raman spectroscopy, as well...

Relations entre les tungstophosphates apparentés à l'anion PW12O403−. Synthèse et propriétés d'un nouveau polyoxotungstophosphate lacunaire K10P2W20O70•24H2O

1987 ◽  
Vol 65 (3) ◽  
pp. 568-573 ◽  
Author(s):  
Roland Contant
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Raman Spectroscopy ◽  
Magnetic Resonance ◽  
Type B ◽  
Compound K ◽  
Nuclear Magnetic Resonance Spectra ◽  
Infrared And Raman Spectroscopy ◽  
Coordination Isomers ◽  
The Relationship ◽  
Magnetic Resonance Spectra

The relationship between all the polyoxotungstophosphates related to PW12O403− and P2W21O716− anions was established. A new compound K10P2W20O70 was synthesized and characterized by infrared and Raman spectroscopy. These properties, and especially the 31P and 183W nuclear magnetic resonance spectra, allowed us to propose the structure. It is a lacunary species that reacts with Co(II) to give two 1–1 complexes, probably coordination isomers. In the presence of Co(II) the A-type → B-type PW9 isomerization occurs in several steps, which are specified. The tungstophosphate β isomers are less stable than the corresponding tungstosilicates or germanates, and β-PW12 was only identified as a reduced species PW12O404−.

scholarly journals Site-Specific Modification and Segmental Isotope Labelling of HMGN1 Reveals Long-Range Conformational Perturbations Caused by Posttranslational Modifications

2020 ◽  
Author(s):  
Gerhard Niederacher ◽  
Debra Urwin ◽  
David J. Tremethick ◽  
K. Johan Rosengren ◽  
Christian F. W. Becker ◽  
...  
Keyword(s):  
Posttranslational Modifications ◽  
High Mobility ◽  
Intrinsically Disordered Protein ◽  
Site Specific ◽  
Isotope Labelling ◽  
Specific Modification ◽  
Intrinsically Disordered ◽  
Cellular Localisation ◽  
And Function ◽  
Nucleosome Binding

Interactions between histones, which package DNA in eukaryotes, and nuclear proteins such as the high mobility group nucleosome-binding protein HMGN1 are important for regulating access to DNA. HMGN1 is a highly charged and intrinsically disordered protein (IDP) that is modified at several sites by posttranslational modifications (PTMs) - acetylation, phosphorylation and ADP-ribosylation. These PTMs are thought to affect cellular localisation of HMGN1 and its ability to bind nucleosomes; however, little is known about how these PTMs regulate the structure and function of HMGN1 at a molecular level. Here, we combine the chemical biology tools of protein semi-synthesis and site-specific modification to generate a series of unique HMGN1 variants bearing precise PTMs at their N- and C-termini with segmental isotope labelling for NMR spectroscopy. This study demonstrates the power of combining protein semi-synthesis for introduction of site-specific PTMs with segmental isotope labelling for structural biology, allowing us to understand the roles of PTMs with atomic precision, from both structural and functional perspectives.<br>

Formation, Structure, and Properties of Nitrosonium and Nitronium Fluorosulfate

1971 ◽  
Vol 49 (1) ◽  
pp. 35-44 ◽  
Author(s):  
A. M. Qureshi ◽  
H. A. Carter ◽  
F. Aubke
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Raman Spectroscopy ◽  
Structure And Properties ◽  
Unit Cell Parameters ◽  
Monoclinic System ◽  
X Ray ◽  
Cell Parameters ◽  
Infrared And Raman Spectroscopy ◽  
Ionic Compounds ◽  
Intermediate Oxidation

The reactions of the nitrogen oxides, N2O, NO, N2O3, NO2 (N2O4), and N2O5, the oxychlorides ClNO and ClNO2, and the oxyanions NO2− and NO3− with the fluorosulfonating agents S2O6F2 and BrOSO2F are studied systematically in regard to the formation of nitrosonium and nitronium fluorosulfate. Complex reactions are found when N2O3 and N2O4 are used as substrates, resulting in mixed or impure products. An explanation involving the intermediate oxidation into N2O5 is put forward to explain the results.The solid products NOSO3F and NO2SO3F are characterized by powder X-ray analysis, infrared and Raman spectroscopy, and their solution behavior in HSO3F. Whereas NOSO3F has been found to have an orthorhombic lattice, NO2SO3F crystallizes in the monoclinic system. Unit cell parameters are reported. The vibrational spectra are interpreted in terms of ionic compounds NO+SO3F− and NO2+ − SO3F−. The SO3F− anion, however, appears to be perturbed and all the E modes are slightly split. No unambiguous evidence for a previously reported non-linear NO2+ cation is found. Force constants for this species are calculated assuming D∞h symmetry.Both compounds are found to be completely dissociated in HSO3F as evidenced by conductometry and 1H and 19F nuclear magnetic resonance (n.m.r.) spectroscopy.

Characterization of fine particulate matter in ambient air by combining TEM and multiple spectroscopic techniques – NMR, FTIR and Raman spectroscopy

2015 ◽  
Vol 17 (3) ◽  
pp. 552-560 ◽  
Author(s):  
Zhurun Ji ◽  
Rucheng Dai ◽  
Zengming Zhang
Keyword(s):  
Raman Spectroscopy ◽  
Fine Particulate Matter ◽  
Ambient Air ◽  
Spectroscopic Techniques ◽  
Fine Particulate ◽  
Transmission Electron ◽  
Spectroscopic Characteristics ◽  
Infrared And Raman Spectroscopy ◽  
Ftir And Raman Spectroscopy

We report a study of the microstructures and spectroscopic characteristics of PM2.5and its potential sources in Beijing by combining transmission electron microscopy and multiple spectroscopic techniques: nuclear magnetic resonance, Fourier transform infrared and Raman spectroscopy.

scholarly journals Extra-Framework Content in Sodalite-Group Minerals: Complexity and New Aspects of Its Study Using Infrared and Raman Spectroscopy

Minerals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 363 ◽  
Author(s):  
Nikita V. Chukanov ◽  
Marina F. Vigasina ◽  
Natalia V. Zubkova ◽  
Igor V. Pekov ◽  
Christof Schäfer ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Raman Spectroscopy ◽  
Structural Analysis ◽  
Ir Spectroscopy ◽  
Quantitative Method ◽  
Fluoride Anion ◽  
Spectroscopy Data ◽  
Group Mineral ◽  
X Ray ◽  
Infrared And Raman Spectroscopy

Nine samples of carbonate-free sodalite-group minerals, including those with abnormally high contents of polysulfide groups, fluoride anion and carbon dioxide molecules as well as synthetic fluoraluminate sodalite-type compound Na8(Si7Al5O24)(AlF6)3–·5H2O, have been studied by means of electron microprobe analyses, infrared and Raman spectroscopy; the CO2 content was determined using the selective sorption of gaseous ignition products. This article describes a semi-quantitative method for estimating the content of carbon dioxide molecules in these minerals, based on IR spectroscopy data. The data obtained demonstrate the existence of a sulfide sodalite-group mineral with the idealized formula Na7(Si6Al6O24)(S3−)·H2O, which differs significantly from the formula Na6Ca2(Si6Al6O24)S2–2 accepted for lazurite. According to single-crystal X-ray structural analysis, in the F-rich sodalite-group mineral from the Eifel paleovolcanic region, Germany with the idealized formula Na7(Si6Al6O24)F−·nH2O fluorine occurs as an isolated F− anion, unlike synthetic F-rich sodalite-type compounds.

scholarly journals Site-Specific Modification and Segmental Isotope Labelling of HMGN1 Reveals Long-Range Conformational Perturbations Caused by Posttranslational Modifications

2020 ◽  
Author(s):  
Gerhard Niederacher ◽  
Debra Urwin ◽  
David J. Tremethick ◽  
K. Johan Rosengren ◽  
Christian F. W. Becker ◽  
...  
Keyword(s):  
Posttranslational Modifications ◽  
High Mobility ◽  
Intrinsically Disordered Protein ◽  
Site Specific ◽  
Isotope Labelling ◽  
Specific Modification ◽  
Intrinsically Disordered ◽  
Cellular Localisation ◽  
And Function ◽  
Nucleosome Binding

Interactions between histones, which package DNA in eukaryotes, and nuclear proteins such as the high mobility group nucleosome-binding protein HMGN1 are important for regulating access to DNA. HMGN1 is a highly charged and intrinsically disordered protein (IDP) that is modified at several sites by posttranslational modifications (PTMs) - acetylation, phosphorylation and ADP-ribosylation. These PTMs are thought to affect cellular localisation of HMGN1 and its ability to bind nucleosomes; however, little is known about how these PTMs regulate the structure and function of HMGN1 at a molecular level. Here, we combine the chemical biology tools of protein semi-synthesis and site-specific modification to generate a series of unique HMGN1 variants bearing precise PTMs at their N- and C-termini with segmental isotope labelling for NMR spectroscopy. This study demonstrates the power of combining protein semi-synthesis for introduction of site-specific PTMs with segmental isotope labelling for structural biology, allowing us to understand the roles of PTMs with atomic precision, from both structural and functional perspectives.<br>

Forensic investigation of arson residue by infrared and Raman spectroscopy: From conventional to non-destructive techniques

2020 ◽  
Vol 60 (3) ◽  
pp. 206-215 ◽  
Author(s):  
Vijay Kumar Yadav ◽  
Kriti Nigam ◽  
Ankit Srivastava
Keyword(s):  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Analytical Techniques ◽  
Chemical Degradation ◽  
Spectroscopic Techniques ◽  
Original Material ◽  
Infrared And Raman Spectroscopy ◽  
Ignitable Liquids ◽  
Fire Debris ◽  
Non Destructive

Arson can result in highly challenging and complicated crime scenes. Much physical evidence undergoes chemical degradation because of the destructive nature of fire, while accelerants either completely burn or evaporate, and may be present in traces within any of the decomposed materials. To identify the original material and the accelerant involved, it is necessary to use advanced analytical techniques. Gas chromatography, with different detectors, is one of the most frequently used instruments in fire debris and accelerant analysis. Among other instruments, capillary electrophoresis and laser-induced thermal desorption Fourier transform mass spectrometry are two major contributors. Vibrational spectroscopy, including infrared absorption and Raman scattering, is one of the major non-destructive tools for the analysis of evidence because of its advantages over other spectroscopic techniques. Most studies involving vibrational spectroscopy (i.e. infrared and Raman spectroscopy) have focused on the identification of commonly found household materials, while very few studies have considered the identification of ignitable liquids. This article reviews studies based on an analysis of fire debris and accelerants by vibrational spectroscopic techniques and considers the limitations and future perspectives of arson investigations in forensic science.

scholarly journals Site-specific modification and segmental isotope labelling of HMGN1 reveals long-range conformational perturbations caused by posttranslational modifications

2021 ◽  
Author(s):  
Gerhard Niederacher ◽  
Debra Urwin ◽  
Yasmin Dijkwel ◽  
David J. Tremethick ◽  
K. Johan Rosengren ◽  
...  
Keyword(s):  
Long Range ◽  
Posttranslational Modifications ◽  
Binding Protein ◽  
Site Specific ◽  
Isotope Labelling ◽  
Specific Modification ◽  
Nucleosome Binding

Using protein semi-synthesis, segmentally isotope-labelled variants of nucleosome-binding protein HMGN1 were generated with site-specific posttranslational modifications to explore their structural and functional effects.

Identification of filler substances in emeralds by infrared and Raman spectroscopy

2000 ◽  
Vol 26 (1) ◽  
pp. 501-520 ◽  
Author(s):  
L. Kiefert ◽  
H.A. Hänni ◽  
J-P. Chalain ◽  
W. Weber
Keyword(s):  
Raman Spectroscopy ◽  
Infrared And Raman Spectroscopy
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