Planar organization of airway epithelial cell morphology using hydrogel grooves during ciliogenesis fails to induce ciliary alignment

2021 ◽  
Author(s):  
Ratna Varma ◽  
James Poon ◽  
Zhongfa Liao ◽  
Stewart Aitchison ◽  
Thomas K Waddell ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Morphology ◽  
Airway Epithelial Cell ◽  
Epithelial Lining ◽  
Airway Epithelial ◽  
Collagen Fibres ◽  
The Matrix ◽  
Cell Organization ◽  
Topographical Cues

Topographical cues are known to influence cell organization both in native tissues and in vitro. In the trachea, the matrix beneath the epithelial lining is composed of collagen fibres that...

Effects of corticosteroid-induced apoptosis on airway epithelial wound closure in vitro

2006 ◽  
Vol 291 (4) ◽  
pp. L794-L801 ◽  
Author(s):  
Delbert R. Dorscheid ◽  
Benjamin J. Patchell ◽  
Oscar Estrada ◽  
Bertha Marroquin ◽  
Roberta Tse ◽  
...  
Keyword(s):  
Cell Migration ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Untreated Control ◽  
Wound Closure ◽  
Airway Epithelial Cell ◽  
Airway Epithelial ◽  
Wound Area ◽  
Epithelial Cell Migration

Damage to the airway epithelium is common in asthma. Corticosteroids induce apoptosis in and suppress proliferation of airway epithelial cells in culture. Whether apoptosis contributes to impaired epithelial cell repair after injury is not known. We examined whether corticosteroids would impair epithelial cell migration in an in vitro model of wound closure. Wounds (∼0.5–1.3 mm2) were created in cultured 1HAEo−human airway epithelial cell monolayers, after which cells were treated with up to 10 μM dexamethasone or budesonide for 24 h. Cultured cells were pretreated for 24 or 48 h with dexamethasone to observe the effect of long-term exposure on wound closure. After 12 h, the remaining wound area in monolayers pretreated for 48 h with 10 μM dexamethasone was 43 ± 18% vs. 10 ± 8% for untreated control monolayers. The addition of either corticosteroid immediately after injury did not slow closure significantly. After 12 h the remaining wound area in monolayers treated with 10 μM budesonide was 39 ± 4% vs. 43 ± 3% for untreated control monolayers. The proportion of apoptotic epithelial cells as measured by terminal deoxynucleotidyltransferase-mediated dUTP biotin nick end labeling both at and away from the wound edge was higher in monolayers treated with budesonide compared with controls. However, wound closure in the apoptosis-resistant 1HAEo−.Bcl-2+cell line was not different after dexamethasone treatment. We demonstrate that corticosteroid treatment before mechanical wounding impairs airway epithelial cell migration. The addition of corticosteroids after injury does not slow migration, despite their ability to induce apoptosis in these cells.

scholarly journals The Function of Ophiocordyceps sinensis in Airway Epithelial Cell Senescence in a Rat COPD Model

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Xiaohui Ma ◽  
Xingai Jiao ◽  
Jinxiang Wu ◽  
Jiping Zhao ◽  
Yurong Xu ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Quantitative Pcr ◽  
Airway Epithelial Cell ◽  
Cell Senescence ◽  
Airway Epithelial ◽  
Airway Epithelia ◽  
Real Time Quantitative Pcr ◽  
Ophiocordyceps Sinensis

Ophiocordyceps sinensis (O. sinensis) seems to be able to alleviate airway epithelial cell senescence in chronic obstructive pulmonary disease (COPD). The objective of the study is to evaluate the effect of O. sinensis on airway epithelial senescence in the COPD model both in vitro and in vivo. We observed the expression of P16 and P21 in the airway epithelia of 30 patients with COPD. The optimal concentration of O. sinensis and exposure time of the cigarette smoke extract (CSE) were determined in vitro, and senescence-associated β-galactosidase (SA-β-gal) and 5-bromodeoxyuridine (BrdU) were used to evaluate the senescence and proliferation of human bronchial epithelial (16HBE) cells pretreated with O. sinensis by staining kits. COPD model rats were treated with O. sinensis at various concentrations to determine the changes in P16 and P21 expression in airway epithelial tissues. It was found that the expression levels of P16 and P21 were higher in the airway epithelia of COPD patients than those in the control group based on immunohistochemical staining, real-time quantitative PCR, and western blotting. The CSE could induce 16HBE cell senescence, and O. sinensis could alleviate CSE-induced senescence and promote the proliferation of 16HBE cells. The expression levels of P16 and P21 were also higher in the airway epithelia of COPD model rats; however, the levels of P16 and P21 in the groups treated with all concentrations of O. sinensis were obviously lower than those in the COPD model group based on real-time quantitative PCR and western blotting. In conclusion, the CSE can induce airway epithelium senescence, and O. sinensis can inhibit CSE-induced cellular senescence, both in vitro and in vivo.

Primary bronchial airway epithelial cell in vitro responses to common environmental exposures

2015 ◽  
Author(s):  
Steve Turner ◽  
Neil McInnes ◽  
Matthew Davidson ◽  
Alison Scaife ◽  
David Miller ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Airway Epithelial Cell ◽  
Environmental Exposures ◽  
Airway Epithelial

scholarly journals Primary Paediatric Bronchial Airway Epithelial Cell in Vitro Responses to Environmental Exposures

2016 ◽  
Vol 13 (4) ◽  
pp. 359 ◽  
Author(s):  
Neil McInnes ◽  
Matthew Davidson ◽  
Alison Scaife ◽  
David Miller ◽  
Daniella Spiteri ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Airway Epithelial Cell ◽  
Environmental Exposures ◽  
Airway Epithelial

scholarly journals Rescue of CF airway epithelial cell function in vitro by a CFTR potentiator, VX-770

2009 ◽  
Vol 8 ◽  
pp. S17
Author(s):  
F. Van Goor ◽  
B. Burton ◽  
S. Hadida ◽  
P. Grootenhuis ◽  
E.R. Olson ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Function ◽  
Airway Epithelial Cell ◽  
Airway Epithelial
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