Preparation of WPU-based super-amphiphobic coatings functionalized by in situ modified SiOx particles and their anti-biofilm mechanism

2021 ◽  
Author(s):  
Jiatao Zhang ◽  
Junyi Yang ◽  
Qiuying Li ◽  
Jie Ding ◽  
Liangjun Liu ◽  
...  
Keyword(s):  
Shewanella Putrefaciens ◽  
Easy Method

TWPU-based super-amphiphobic coatings functionalized by in situ modified SiOx particles were developed by an easy method for revealing their anti-biofilm mechanism to Shewanella putrefaciens.

scholarly journals In Situ Spectral Kinetics of Cr(VI) Reduction by c-Type Cytochromes in A Suspension of Living Shewanella putrefaciens 200

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Tongxu Liu ◽  
Xiaomin Li ◽  
Fangbai Li ◽  
Rui Han ◽  
Yundang Wu ◽  
...  
Keyword(s):  
Shewanella Putrefaciens ◽  
Kinetics Of

scholarly journals Detection of Oligodendrocytes in Tissue Sections Using PCR Synthesis of Digoxigenin-labeled Probes

2003 ◽  
Vol 51 (7) ◽  
pp. 913-919 ◽  
Author(s):  
Walid Jalabi ◽  
Mirela Cerghet ◽  
Robert P. Skoff ◽  
M. Said Ghandour
Keyword(s):  
Nervous System ◽  
Proteolipid Protein ◽  
Newborn Mouse ◽  
Specific Primers ◽  
Easy Method ◽  
Double Labeling ◽  
Tissue Sections ◽  
The Central Nervous System ◽  
Specific Mrnas

Oligodendrocytes, the myelin-forming cells in the central nervous system, were visualized with excellent resolution at the light microscopic level using in situ hybridization (ISH). Digoxigenin (Dig)-tagged probes were synthesized and efficiently labeled by PCR. Specific probes to myelin genes were made by RT from brain total RNAs, followed by PCR with designed specific primers in the presence of Dig-11-dUTP. Probes specific to proteolipid protein (PLP), PLP and its isoform DM20 (PLP/DM20), and myelin oligodendrocyte glycoprotein (MOG) were synthesized and labeled. ISH was then applied on vibratomed tissue sections from mouse brains. Despite a low expression of MOG-specific and PLP-specific mRNAs in adult and newborn mouse brains, an oligodendrocyte population was detected. The specificity of Dig-labeled probes was confirmed with the double labeling of carbonic anhydrase II (CA II) and glial fibrillary acidic protein (GFAP) immunocytochemistry and ISH. This versatile and easy method for synthesis and labeling of specific probes to oligodendrocytes can be also applied to detect many other mRNAs in the nervous system and in other tissues.

scholarly journals In Situ Formation of Ionic Liquid by Metathesis Reaction for the Rapid Removal of Bisphenol A from Aqueous Solutions

Water ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2087 ◽  
Author(s):  
Yesica Vicente-Martínez ◽  
Manuel Caravaca ◽  
Antonio Soto-Meca ◽  
Óscar De Francisco-Ortíz ◽  
Carmen Fernández-López
Keyword(s):  
Ionic Liquid ◽  
Bisphenol A ◽  
Aqueous Solutions ◽  
High Performance ◽  
Cost Effective ◽  
Metathesis Reaction ◽  
Experimental Conditions ◽  
Easy Method ◽  
Dispersive Liquid Liquid Microextraction

In this work we present a rapid and easy method to remove the totality of bisphenol A from aqueous solutions using ionic liquid (IL). Dispersive liquid–liquid microextraction is employed. The IL 1-octyl-3-methylimidazolium bis((trifluoromethane)sulfonyl)imide ([C8C1im] [NTf2]) is formed in situ because of the mixture of 1-octyl-3-methylimidazolium chloride ([C8C1im]Cl) and lithium bis(trifluoromethanesulfonyl)imide (Li[NTf2]) aqueous solutions. A cloud of microdroplets of IL formed by the dispersion generated through the precursors metathesis reaction allows the rapid and total extraction of bisphenol A (BPA). After centrifugation, the formed IL phase is deposited at the bottom of the flask and the total amount of BPA is extracted in the sedimented phase. The volume of IL is very low, in the order of microliters, which enables us to remove all the BPA from the solution. The technique studied is highly efficient, cost-effective, and presents less environmental impact than other extraction techniques, thus becoming an outstanding alternative to the most commonly used methods. BPA concentration is determined by high performance liquid chromatography by injecting the IL phase directly. An extraction kinetic model for the kinetic profile has been tested for this method, which allows to infer the ideal experimental conditions to execute the extraction method.

scholarly journals Ladder-type sulfonated poly(arylene perfluoroalkylene)s for high performance proton exchange membrane fuel cells

RSC Advances ◽  
2020 ◽  
Vol 10 (67) ◽  
pp. 41058-41064
Author(s):  
Zhi Long ◽  
Junpei Miyake ◽  
Kenji Miyatake
Keyword(s):  
Fuel Cell ◽  
High Performance ◽  
Proton Exchange Membrane ◽  
Mechanical Stability ◽  
Proton Exchange ◽  
Easy Method ◽  
Fuel Cell Performance ◽  
Exchange Membrane ◽  
Sulfonated Poly

Sulfone-bonded ladder-type sulfonated poly(arylene perfluoroalkylene)s (SPAF-P-Lad) were synthesized by an easy method to achieve high thermo-mechanical stability, proton conductivity, fuel cell performance and remarkable in situ durability.

Kinetics of Fe(III) and Mn(IV) reduction by the Black Sea strain of Shewanella putrefaciens using in situ solid state voltammetric Au/Hg electrodes

2000 ◽  
Vol 70 (1-3) ◽  
pp. 171-180 ◽  
Author(s):  
Michael E Dollhopf ◽  
Kenneth H Nealson ◽  
Dawn M Simon ◽  
George W Luther
Keyword(s):  
Solid State ◽  
Black Sea ◽  
Shewanella Putrefaciens ◽  
The Black Sea ◽  
Kinetics Of

Development of an easy method to test for HER2 in breast cancer using dual-color in situ hybridization

Breast Cancer ◽  
2014 ◽  
Vol 23 (1) ◽  
pp. 78-84
Author(s):  
Tomoe Nakagawa ◽  
Rie Horii ◽  
Yoshinori Ito ◽  
Takuji Iwase ◽  
Futoshi Akiyama
Keyword(s):  
Breast Cancer ◽  
In Situ Hybridization ◽  
Easy Method ◽  
Dual Color

In situ fixation of the spinal cord using microwave radiation

1988 ◽  
Vol 69 (5) ◽  
pp. 719-722 ◽  
Author(s):  
David J. Gower ◽  
Carol Hollman ◽  
K. Stuart Lee ◽  
Michael Tytell
Keyword(s):  
Spinal Cord ◽  
Mechanical Trauma ◽  
Tissue Fixation ◽  
Easy Method ◽  
Content Type ◽  
In Situ Fixation ◽  
Tissue Degradation ◽  
Perfusion Fixation ◽  
Fine Print

✓ Due to its investiture with bone, the spinal cord can be difficult to study anatomically and histologically. Tissue degradation during immersion fixation or mechanical trauma during extraction of unfixed tissue often produces confusing artifacts. Perfusion fixation eliminates many of these problems, but it is a slow, tedious, and technically demanding procedure. This report demonstrates that microwave irradiation of the spinal cord before its removal from the spine is a rapid and easy method of tissue fixation with an absence of artifacts comparable to that with perfusion fixation.

DNA replication-dependent intranuclear relocation of double minute chromatin

1998 ◽  
Vol 111 (22) ◽  
pp. 3275-3285 ◽  
Author(s):  
N. Itoh ◽  
N. Shimizu
Keyword(s):  
Dna Replication ◽  
Nuclear Periphery ◽  
Simultaneous Detection ◽  
Nuclear Lamina ◽  
Easy Method ◽  
Circular Dna ◽  
Growth And Survival ◽  
M Phase ◽  
Cell Fixation

Double minutes (DMs) seen in a substantial fraction of human tumors are the cytogenetic manifestation of gene amplification which renders the tumor cells advantageous for growth and survival. DMs are acentric and atelomeric chromatin composed of circular DNA. In this study, we found they showed a remarkable relocation inside the nucleus which was spatially and temporally coupled to DNA replication. Using the human COLO 320DM tumor line, we detected DMs by fluorescence in situ hybridization followed by confocal examination. The location of multi-copy DMs was evaluated statistically by an easy method developed in this study. By examination of a synchronized culture, we found that DMs preferentially located at the nuclear periphery during G1-phase of the cell cycle, which is consistent with the location at M-phase. The peripheral DMs were in contact with the nuclear lamina which was shown by the simultaneous detection of DMs and lamin protein. The peripheral location persisted until the cells reached the G1/S-boundary, then the DMs relocated promptly to inward once the DNA replication started. The relocation was obvious using two different probes that detect DMs, or using two different methods for the cell fixation. Furthermore, the simultaneous detection of DMs and the site of DNA replication suggested that the inward relocation of peripheral DMs initiated just prior to the onset of DNA replication at the periphery. On the other hand, if the same amplified sequences were placed in a chromosome as an homogeneously staining region, they did not show any significant relocation during S-phase. From these and reported results, there may exist a generalized inward motion of some kind of chromatin that precedes the replication of their DNA. DMs might magnify the motion by their acentric, atelomeric or small circular nature.

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