Development of an Eco-friendly Sample Preparation protocol for metals determination in food samples: Oxygen Pressurized Single Reaction Chamber using Diluted Nitric Acid

2021 ◽  
Author(s):  
Cezar Augusto Bizzi ◽  
Matheus Felipe Pedrotti ◽  
Diogo Maurício Betiolo ◽  
Mariele Samuel Nascimento ◽  
Edson Irineu Müller ◽  
...  
Keyword(s):  
Nitric Acid ◽  
Sample Preparation ◽  
Reaction Chamber ◽  
Food Samples ◽  
Alternative Method ◽  
Digestion Efficiency ◽  
Single Reaction

Many efforts have been recently made to improve the digestion efficiency by using powerful equipment or by using auxiliary reagent. In this work, an alternative method is reported, which explores...

scholarly journals Rapid Method Using Two Microbial Enzymes for Detection of l-Abrine in Food as a Marker for the Toxic Protein Abrin

2014 ◽  
Vol 81 (5) ◽  
pp. 1610-1615 ◽  
Author(s):  
Anthony G. Dodge ◽  
Kelvin Carrasquillo ◽  
Luis Rivera ◽  
Lei Xu ◽  
Lawrence P. Wackett ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Visual Detection ◽  
Food Samples ◽  
Content Type ◽  
Toxic Protein ◽  
Beverage Samples ◽  
Lower Detection Limit ◽  
Lower Detection ◽  
Complex Sample ◽  
Food And Beverage

ABSTRACTAbrin is a toxic protein produced by the ornamental plantAbrus precatorius, and it is of concern as a biothreat agent. The small coextracting moleculeN-methyl-l-tryptophan (l-abrine) is specific to members of the genusAbrusand thus can be used as a marker for the presence or ingestion of abrin. Current methods for the detection of abrin orl-abrine in foods and other matrices require complex sample preparation and expensive instrumentation. To develop a fast and portable method for the detection ofl-abrine in beverages and foods, theEscherichia coliproteinsN-methyltryptophan oxidase (MTOX) and tryptophanase were expressed and purified. The two enzymes jointly degradedl-abrine to products that included ammonia and indole, and colorimetric assays for the detection of those analytes in beverage and food samples were evaluated. An indole assay using a modified version of Ehrlich's/Kovac's reagent was more sensitive and less subject to negative interferences from components in the samples than the Berthelot ammonia assay. The two enzymes were added into food and beverage samples spiked withl-abrine, and indole was detected as a degradation product, with the visual lower detection limit being 2.5 to 10.0 μM (∼0.6 to 2.2 ppm)l-abrine in the samples tested. Results could be obtained in as little as 15 min. Sample preparation was limited to pH adjustment of some samples. Visual detection was found to be about as sensitive as detection with a spectrophotometer, especially in milk-based matrices.

scholarly journals Sample preparation combined with electroanalysis to improve simultaneous determination of antibiotics in animal derived food samples

2018 ◽  
Vol 250 ◽  
pp. 7-13 ◽  
Author(s):  
Wesley Pereira da Silva ◽  
Luiz Henrique de Oliveira ◽  
André Luiz dos Santos ◽  
Valdir Souza Ferreira ◽  
Magno Aparecido Gonçalves Trindade
Keyword(s):  
Sample Preparation ◽  
Simultaneous Determination ◽  
Food Samples

Rare earth element determination in heavy crude oil by USN-ICP-MS after digestion using a microwave-assisted single reaction chamber

2016 ◽  
Vol 31 (6) ◽  
pp. 1185-1191 ◽  
Author(s):  
Gabriel T. Druzian ◽  
Leticia S. F. Pereira ◽  
Paola A. Mello ◽  
Marcia F. Mesko ◽  
Fabio A. Duarte ◽  
...  
Keyword(s):  
Rare Earth ◽  
Crude Oil ◽  
Rare Earth Element ◽  
Earth Element ◽  
Reaction Chamber ◽  
Heavy Crude Oil ◽  
Microwave Assisted ◽  
Icp Ms ◽  
Heavy Crude ◽  
Single Reaction

In this work a method for rare earth element (REE) determination by inductively coupled plasma mass spectrometry (ICP-MS) was proposed after heavy crude oil digestion by microwave-assisted wet digestion (MAWD) using a single reaction chamber (SRC) system.

scholarly journals Development of the multiplex system for determining markers of predisposition to cardiovascular diseases

2021 ◽  
Vol 57 (1) ◽  
pp. 48-60
Author(s):  
I. V. Haidukevich ◽  
A. M. Gorkavaya ◽  
A. V. Grudo ◽  
G. V. Sergeev ◽  
S. A. Usanov
Keyword(s):  
Cardiovascular Diseases ◽  
Sample Preparation ◽  
High Sensitivity ◽  
Fragment Analysis ◽  
Different Types ◽  
Multiplex System ◽  
Single Reaction

A multiplex system to detect mutations at loci rs5985, rs1799983, rs5918, rs2243093, rs4673, rs4646994, rs1722009, rs3980933, rs71103505 associated with the development of cardiovascular diseases has been developed. These mutations belong to different types - SNP, STR, Ins/Del - therefore, minisequencing and fragment analysis technologies were used to determine them. Oligonucleotide analysis was performed to amplify all loci in a single reaction format. The minisequencing technology, in comparison with fragment analysis, required additional stages of sample preparation; therefore, oligonucleotides for loci with SNP were combined into a separate plex. For the two plexes created, the same composition of the amplification mixture and parameters of the PCR reaction program were optimized, and “bin” panels were developed to interpret the results. The testing of the system confirmed the possibility of detecting mutations at nine loci with high sensitivity and reproducibility. 

Use of Microwave Oven and Nitric Acid-Hydrogen Peroxide Digestion to Prepare Botanical Materials for Elemental Analysis by Inductively Coupled Argon Plasma Emission Spectroscopy

1985 ◽  
Vol 68 (4) ◽  
pp. 766-769
Author(s):  
Thomas R White ◽  
Garnett E Douthit
Keyword(s):  
Hydrogen Peroxide ◽  
Nitric Acid ◽  
Sample Preparation ◽  
Emission Spectroscopy ◽  
Argon Plasma ◽  
Microwave Oven ◽  
Standard Reference Materials ◽  
Plasma Emission ◽  
Inductively Coupled ◽  
Plasma Emission Spectroscopy

Abstract A sample preparation method has been developed that provides a nitric acid-hydrogen peroxide digestion of plant tissue when it is heated internally by the oscillating electromagnetic field generated in a microwave oven. The total elemental concentration for barium, calcium, magnesium, manganese, phosphorus, potassium, sodium, sulfur, and zinc can be determined by inductively coupled argon plasma emission spectroscopy after sample preparation in a microwave oven. The method is rapid and provides quantitative results that agree with certified values for 4 National Bureau of Standards Standard Reference Materials: 1570 Spinach, 1572 Citrus Leaves, 1573 Tomato Leaves, and 1575 Pine Needles.

scholarly journals Multivariate Assessment of Procedures for Molecularly Imprinted Polymer Synthesis for Pesticides Determination in Environmental and Agricultural Samples

Materials ◽  
2021 ◽  
Vol 14 (22) ◽  
pp. 7078
Author(s):  
Mariusz Marć ◽  
Marta Bystrzanowska ◽  
Katarzyna Pokajewicz ◽  
Marek Tobiszewski
Keyword(s):  
Sample Preparation ◽  
Solid Phase ◽  
Food Samples ◽  
Mechanical Resistance ◽  
Molecularly Imprinted ◽  
Innovative Strategies ◽  
Wide Range ◽  
Analytical Procedures ◽  
Pre Treatment ◽  
The Impact

In the case of quantitative and qualitative analysis of pesticides in environmental and food samples, it is required to perform a sample pre-treatment process. It allows to minimalize the impact of interferences on the final results, as well as increase the recovery rate. Nowadays, apart from routinely employed sample preparation techniques such as solid-phase extraction (SPE) or solid-phase microextraction (SPME), the application of molecularly imprinted polymers (MIPs) is gaining greater popularity. It is mainly related to their physicochemical properties, sorption capacity and selectivity, thermo-mechanical resistance, as well as a wide range of polymerization techniques allowing to obtain the desired type of sorption materials, adequate to a specific type of pesticide. This paper targets to summarize the most popular and innovative strategies since 2010, associated with the MIPs synthesis and analytical procedures for pesticides determination in environmental and food samples. Application of multi-criteria decision analysis (MCDA) allows for visualization of the most beneficial analytical procedures in case of changing the priority of each step of analysis (MIPs synthesis, sample preparation process—pesticides extraction, chromatographic analysis) bearing in mind metrological and environmental issues.

scholarly journals Digital droplet PCR on disk

Lab on a Chip ◽  
2016 ◽  
Vol 16 (1) ◽  
pp. 208-216 ◽  
Author(s):  
Friedrich Schuler ◽  
Martin Trotter ◽  
Marcel Geltman ◽  
Frank Schwemmer ◽  
Simon Wadle ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Reaction Chamber ◽  
Droplet Generation ◽  
Digital Droplet Pcr ◽  
Droplet Pcr ◽  
Single Reaction

An integrated digital droplet PCR system is presented that enables droplet generation, PCR amplification, and readout within a single reaction chamber on a centrifugal microfluidic LabDisk.

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