scholarly journals An automated microfluidic platform for the screening and characterization of novel hepatitis B virus capsid assembly modulators

2022 ◽  
Author(s):  
Tamás Vermes ◽  
Mark Kielpinski ◽  
Thomas Henkel ◽  
Miquel A. Pericàs ◽  
Esther Alza ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
High Resolution ◽  
Hepatitis B ◽  
Dose Response ◽  
Microfluidic System ◽  
Capsid Assembly ◽  
Response Curves ◽  
B Virus ◽  
Convection Dominated

A fully automated microfluidic system was developed to screen for novel anti-HBV capsid assembly modulators. High-resolution dose–response curves were generated using convection-dominated Taylor–Aris dispersion of the screening compounds.

scholarly journals Molecular characterization of hepatitis B virus genotypes in Uzbekistan

2020 ◽  
Vol 101 ◽  
pp. 511
Author(s):  
M. Abdukadirova ◽  
A. Khikmatullaeva ◽  
N. Ibadullaeva ◽  
S. Bakieva
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Molecular Characterization ◽  
B Virus ◽  
Virus Genotypes

scholarly journals Functional Characterization of Interferon Regulation Element of Hepatitis B virus Genome In Vivo

2012 ◽  
Vol 23 (3) ◽  
pp. 278-285 ◽  
Author(s):  
Feng-Jun Liu ◽  
En-Qiang Chen ◽  
Qiao-Ling Zhou ◽  
Tao-You Zhou ◽  
Cong Liu ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Functional Characterization ◽  
Virus Genome ◽  
B Virus

scholarly journals Characterization of antiviral resistance mutations among the Eastern Indian Hepatitis B virus infected population

2013 ◽  
Vol 10 (1) ◽  
pp. 56 ◽  
Author(s):  
Rajesh Panigrahi ◽  
Avik Biswas ◽  
Binay Krishna De ◽  
Sekhar Chakrabarti ◽  
Runu Chakravarty
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Antiviral Resistance ◽  
Resistance Mutations ◽  
Infected Population ◽  
B Virus

scholarly journals Functional association of cellular microtubules with viral capsid assembly supports efficient hepatitis B virus replication

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Masashi Iwamoto ◽  
Dawei Cai ◽  
Masaya Sugiyama ◽  
Ryosuke Suzuki ◽  
Hideki Aizaki ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Virus Replication ◽  
Viral Capsid ◽  
Capsid Assembly ◽  
Functional Association ◽  
B Virus

Characterization of a novel hepatitis B virus subgenotype B10 among chronic hepatitis B patients in Yunnan, China

2020 ◽  
Vol 83 ◽  
pp. 104322
Author(s):  
Ying Liu ◽  
Yue Feng ◽  
Yaling Li ◽  
Jing Ma ◽  
Yuanyuan Jia ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
B Virus

Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody specific for the S antigen of hepatitis B virus

Gene ◽  
1994 ◽  
Vol 144 (2) ◽  
pp. 313-314 ◽  
Author(s):  
Ryu Chun Jeih ◽  
Jin Byung Rae ◽  
Chung Hong Keun ◽  
Han Moon Hi ◽  
Hong Hyo Jeong
Keyword(s):  
Monoclonal Antibody ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Light Chains ◽  
B Virus

scholarly journals Characterization of hepatitis B virus genotypes among Yucpa Indians in Venezuela

2001 ◽  
Vol 82 (2) ◽  
pp. 359-365 ◽  
Author(s):  
Tatsunori Nakano ◽  
Ling Lu ◽  
Xiaolei Hu ◽  
Masashi Mizokami ◽  
Etsuro Orito ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Complete Genome ◽  
Deletion Mutants ◽  
Reading Frame ◽  
B Virus ◽  
Virus Genotypes ◽  
Core Reading ◽  
Genotype F

The complete genome sequences of hepatitis B virus (HBV) from 12 HBV-infected Yucpa Indians of Venezuela, a group with highly endemic HBV, were amplified and sequenced. The 12 isolates were closely related to each other, with 98·6–100% nucleotide identity. A phylogenetic tree based on the complete genome indicated clearly that they were genotype F. Three individuals had evidence of infection with two different HBV deletion mutants. In two individuals, a three amino acid deletion was identified just prior to the ‘a’ determinant loop of the S region. A third individual was infected with virus that contained a complete core reading frame and a population that contained a deletion in the middle of the core region. These results indicate that genotype F HBV is present in the Venezuelan Yucpa Amerindians and the complete genome sequence allowed the identification of two unique deletion mutants in a limited set of samples.

scholarly journals Characterization of a G-quadruplex from hepatitis B virus and its stabilization by binding TMPyP4, BRACO19 and PhenDC3

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Orsolya Réka Molnár ◽  
András Végh ◽  
Judit Somkuti ◽  
László Smeller
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Binding Constants ◽  
Temperature Stability ◽  
Cd Spectroscopy ◽  
Parallel Structure ◽  
Hybrid Form ◽  
B Virus ◽  
G Quadruplex

AbstractSpecific guanine rich nucleic acid sequences can form non-canonical structures, like the four stranded G-quadruplex (GQ). We studied the GQ-forming sequence (named HepB) found in the genome of the hepatitis B virus. Fluorescence-, infrared- and CD-spectroscopy were used. HepB shows a hybrid form in presence of K+, but Na+, Li+, and Rb+ induce parallel structure. Higher concentrations of metal ions increase the unfolding temperature, which was explained by a short thermodynamic calculation. Temperature stability of the GQ structure was determined for all these ions. Na+ has stronger stabilizing effect on HepB than K+, which is highly unusual. The transition temperatures were 56.6, 53.8, 58.5 and 54.4 °C for Na+, K+, Li+, and Rb+ respectively. Binding constants for Na+ and K+ were 10.2 mM and 7.1 mM respectively. Study of three ligands designed in cancer research for GQ targeting (TMPyP4, BRACO19 and PhenDC3) showed unequivocally their binding to HepB. Binding was proven by the increased stability of the bound form. The stabilization was higher than 20 °C for TMPyP4 and PhenDC3, while it was considerably lower for BRACO19. These results might have medical importance in the fight against the hepatitis B virus.

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