Development of a simple and sensitive HPLC-DAD method for quantification of vitamin B12 fortified in infant food

2021 ◽  
Author(s):  
dingyan fan ◽  
yingzhou zhang ◽  
haiping wu
Keyword(s):  
Quantitative Determination ◽  
Vitamin B12 ◽  
Vitamin B12 Deficiency ◽  
Cow’S Milk ◽  
Infant Food ◽  
Cow's Milk ◽  
B12 Deficiency

To prevent infants from vitamin B12 deficiency,infant food is designed based on cow’s milk or cereal with the fortification of vitamin B12.A method for quantitative determination of vitamin B12 in...

scholarly journals Determination of Vitamin B12 Deficiency Status in Vegan and Non-Vegan Thais by Assessment of Homocysteine Level

2021 ◽  
Vol 17 (01) ◽  
pp. 15-19
Author(s):  
Sant Chaiyodsilp ◽  
Sirada Phuriwattanapong ◽  
Weerapong Chaiyaphak ◽  
Somwong Chaiyodsilp ◽  
Phasuk Pruisart
Keyword(s):  
Vitamin B12 ◽  
Homocysteine Level ◽  
Vitamin B12 Deficiency ◽  
B12 Deficiency

scholarly journals Breast milk provides inadequate amounts of vitamin B12 for predominantly breastfed Guatemalan infants

2020 ◽  
Vol 90 (5-6) ◽  
pp. 395-402
Author(s):  
Miriam A. Anaya-Loyola ◽  
Alex Brito ◽  
Kenneth H. Brown ◽  
Lindsay H. Allen
Keyword(s):  
Breast Milk ◽  
Vitamin B12 ◽  
Cow’S Milk ◽  
Guatemala City ◽  
Total Consumption ◽  
Cow's Milk ◽  
High Prevalence ◽  
B12 Deficiency ◽  
And Function ◽  
The Brain

Abstract. Vitamin B12 (B12) plays in an important role in the development and function of the brain and nervous system, and adequate B12 status is especially important for the normal development of infants. In previous research conducted in Guatemala City we reported a high prevalence of B12 deficiency in lactating women and their infants 3 and 12 months of age, and low B12 concentrations in breast milk. The objective of this study was to assess predictors of serum B12 concentration in predominantly breastfed Guatemalan infants including intake of B12 from breast milk and other foods. Serum B12, breast milk and other food intakes, anthropometry, morbidity and socioeconomic status were assessed in infants 6.7 ± 0.6 months of age (n = 127, 52% female) in peri-urban Guatemala City. Twenty-four percent of infants had deficient B12 status (serum B12 concentration < 148 pmol/L) and 37% had marginal B12 status (148–220 pmol/L). Serum B12 concentrations were negatively correlated with infants’ consumption of energy from breast milk (r = −0.37, p = 0.001), and positively correlated with their total consumption of animal source foods, especially cow’s milk (r = 0.40, p = 0.001). Based on previously analyzed breast milk B12 concentrations in a nearby community, breast milk provided < 10% of the recommended daily B12 intake for this age. We conclude that there was a high prevalence of B12 deficiency in these Guatemalan infants by 6 months of age. Serum B12 was higher in infants consuming more cow’s milk and lower in those consuming more breast milk.

Relationships among Urinary Aminoimidazolecarboxamide in Urine and Folate, and Vitamin B12 Concentrations in Serum

1973 ◽  
Vol 19 (9) ◽  
pp. 1049-1052 ◽  
Author(s):  
J W Harrison ◽  
B A Slade ◽  
W Shaw
Keyword(s):  
Vitamin B12 ◽  
Serum Vitamin ◽  
Vitamin B12 Deficiency ◽  
Strong Relationship ◽  
Automated System ◽  
Serum Folate ◽  
B12 Deficiency ◽  
The Mean ◽  
Folate And Vitamin B12

Abstract Urinary aminoimidazolecarboxamide (AIC), serum folate, and serum vitamin B12 values were determined in 84 apparently healthy individuals. An automated system for determination of AIC in urine is described. Despite claims to the contrary, we found no evidence of a strong relationship between elevated (e.g., &gt;1.3 µg/mg of creatinine) AIC excretion as reflected in a casual sample of urine and folate or vitamin B12 deficiency. Urinary AIC values ranged from 0.10 to 5.20 µg/mg of creatinine. The mean for the population examined was 1.36 ± 1.02 µg/mg of creatinine.

scholarly journals Determination of indirect reference intervals of vitamin B12 for outpatients admitted to primary healthcare centers

2020 ◽  
Vol 44 (1) ◽  
pp. 11-16
Author(s):  
Esin Avci ◽  
Diler Aslan
Keyword(s):  
Vitamin B12 ◽  
Primary Healthcare ◽  
Clinical Chemistry ◽  
Age Groups ◽  
Vitamin B12 Deficiency ◽  
Reference Intervals ◽  
Microcytic Anemia ◽  
Reference Limits ◽  
B12 Deficiency

Abstract Background Vitamin B12 deficiency is still an important nutritional problem worldwide. There is no consensus on the reference intervals (RIs) for vitamin B12. In order to establish vitamin B12 RIs, we used the database obtained from a primary healthcare organization and estimated region-specific intervals. Methods Vitamin B12 results were collected from our laboratory information system (LIS) between January 2014 and July 2014. We excluded the results of the individuals who had megaloblastic and microcytic anemia. After outlier detection and exclusion, we estimated the RIs of the “1–12, 13–18, 19–64 and 65 +” age groups according to the International Federation of Clinical Chemistry (IFCC) method. Statistical differences were examined between groups. Results The reference limits and 90% confidence intervals (CIs) (n = 14,748, 3790 male, age: 1–96; 10,958 female, age: 1–115) were estimated with 90% CIs as 106 (104–107)–393 (391–395) pmol/L. We also estimated the reference limits of the “1–12, 13–18, 19–64 and 65 +” age groups to be 127–459, 108–398, 106–384 and 104–426 pmol/L, respectively. There was no difference between genders in all age groups. Conclusions Both the lower and upper limits of the estimated RIs were found to be lower than the reference limits recommended by the manufacturer. Our findings revealed that the RIs of vitamin B12 should be given for the “1–12, 13–18, 19–64 and 65 +” age groups separately. The big data obtained from laboratories should be used for the determination of its population health status. As our population is composed of outpatients, the estimated vitamin B12 RIs may establish the basic information for the examination of our population for vitamin B12 deficiency.

55: The Risk of Vitamin B12 Deficiency is Low after Ileal Orthotopic Bladder Substitution

2004 ◽  
Vol 171 (4S) ◽  
pp. 15-15
Author(s):  
Urs E. Studer ◽  
Richard Aebischer ◽  
Katharina Ochsner ◽  
Werner W. Hochreiter
Keyword(s):  
Vitamin B12 ◽  
Vitamin B12 Deficiency ◽  
B12 Deficiency ◽  
Bladder Substitution

Bioavailability of Vitamin B12

2010 ◽  
Vol 80 (45) ◽  
pp. 330-335 ◽  
Author(s):  
Lindsay Helen Allen
Keyword(s):  
Vitamin B12 ◽  
Vitamin B12 Deficiency ◽  
The Elderly ◽  
Radioactive Isotopes ◽  
Animal Source Foods ◽  
Dietary Requirements ◽  
In Vivo Labeling ◽  
B12 Deficiency ◽  
Vitamin B12 Malabsorption

Vitamin B12 deficiency is common in people of all ages who consume a low intake of animal-source foods, including populations in developing countries. It is also prevalent among the elderly, even in wealthier countries, due to their malabsorption of B12 from food. Several methods have been applied to diagnose vitamin B12 malabsorption, including Schilling’s test, which is now used rarely, but these do not quantify percent bioavailability. Most of the information on B12 bioavailability from foods was collected 40 to 50 years ago, using radioactive isotopes of cobalt to label the corrinoid ring. The data are sparse, and the level of radioactivity required for in vivo labeling of animal tissues can be prohibitive. A newer method under development uses a low dose of radioactivity as 14C-labeled B12, with measurement of the isotope excreted in urine and feces by accelerator mass spectrometry. This test has revealed that the unabsorbed vitamin is degraded in the intestine. The percent bioavailability is inversely proportional to the dose consumed due to saturation of the active absorption process, even within the range of usual intake from foods. This has important implications for the assessment and interpretation of bioavailability values, setting dietary requirements, and interpreting relationships between intake and status of the vitamin.

Increased monocyte to HDL cholesterol ratio in vitamin B12 deficiency: is it related to cardiometabolic risk?

2020 ◽  
pp. 1-8
Author(s):  
Sanem Kayhan ◽  
Nazli Gulsoy Kirnap ◽  
Mercan Tastemur
Keyword(s):  
Risk Factors ◽  
Blood Pressure ◽  
Uric Acid ◽  
Vitamin B12 ◽  
Cardiometabolic Risk ◽  
Vitamin B12 Deficiency ◽  
Left Ventricular ◽  
Cardiometabolic Risk Factors ◽  
B12 Deficiency ◽  
Deficiency Group

Abstract. Vitamin B12 deficiency may have indirect cardiovascular effects in addition to hematological and neuropsychiatric symptoms. It was shown that the monocyte count-to-high density lipoprotein cholesterol (HDL-C) ratio (MHR) is a novel cardiovascular marker. In this study, the aim was to evaluate whether MHR was high in patients with vitamin B12 deficiency and its relationship with cardiometabolic risk factors. The study included 128 patients diagnosed with vitamin B12 deficiency and 93 healthy controls. Patients with vitamin B12 deficiency had significantly higher systolic blood pressure (SBP), diastolic blood pressure (DBP), MHR, C-reactive protein (CRP) and uric acid levels compared with the controls (median 139 vs 115 mmHg, p < 0.001; 80 vs 70 mmHg, p < 0.001; 14.2 vs 9.5, p < 0.001; 10.2 vs 4 mg/dl p < 0.001; 6.68 vs 4.8 mg/dl, p < 0.001 respectively). The prevalence of left ventricular hypertrophy was higher in vitamin B12 deficiency group (43.8%) than the control group (8.6%) (p < 0.001). In vitamin B12 deficiency group, a positive correlation was detected between MHR and SBP, CRP and uric acid (p < 0.001 r:0.34, p < 0.001 r:0.30, p < 0.001 r:0.5, respectively) and a significant negative correlation was detected between MHR and T-CHOL, LDL, HDL and B12 (p < 0.001 r: −0.39, p < 0.001 r: −0.34, p < 0.001 r: −0.57, p < 0.04 r: −0.17, respectively). MHR was high in vitamin B12 deficiency group, and correlated with the cardiometabolic risk factors in this group, which were SBP, CRP, uric acid and HDL. In conclusion, MRH, which can be easily calculated in clinical practice, can be a useful marker to assess cardiovascular risk in patients with vitamin B12 deficiency.

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