Highly stable surface-enhanced Raman spectroscopy assay on abnormal thrombin levels in the blood plasma of cancer patients

2021 ◽  
Vol 13 (37) ◽  
pp. 4328-4333
Author(s):  
Lingling Liao ◽  
Shanshan Du ◽  
Yan Ding ◽  
Mengke Su ◽  
Ting Yu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Blood Plasma ◽  
Cancer Patients ◽  
False Positive ◽  
Surface Enhanced Raman Spectroscopy ◽  
Clinical Diagnostics ◽  
Dual Reporter ◽  
Surface Enhanced ◽  
Testing Tool ◽  
Surface Enhanced Raman

A double-aptamer sandwich strategy for dual-reporter SERS assay with reduced false-positive as practical protein testing tool in clinical diagnostics and prognosis.

scholarly journals Toward Cancer Diagnostics of the Tumor Suppressor p53 by Surface Enhanced Raman Spectroscopy

Sensors ◽  
2020 ◽  
Vol 20 (24) ◽  
pp. 7153
Author(s):  
Anna Rita Bizzarri ◽  
Salvatore Cannistraro
Keyword(s):  
Raman Spectroscopy ◽  
Tumor Suppressor ◽  
P53 Protein ◽  
Surface Enhanced Raman Spectroscopy ◽  
Cancer Diagnostics ◽  
Clinical Diagnostics ◽  
Tumor Suppressor P53 ◽  
Wild Type ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

The tumor suppressor p53 protein plays a crucial role in many biological processes. The presence of abnormal concentrations of wild-type p53, or some of its mutants, can be indicative of a pathological cancer state. p53 represents therefore a valuable biomarker for tumor screening approaches and development of suitable biosensors for its detection deserves a high interest in early diagnostics. Here, we revisit our experimental approaches, combining Surface Enhanced Raman Spectroscopy (SERS) and nanotechnological materials, for ultrasensitive detection of wild-type and mutated p53, in the perspective to develop biosensors to be used in clinical diagnostics. The Raman marker is provided by a small molecule (4-ATP) acting as a bridge between gold nanoparticles (NPs) and a protein biomolecule. The Azurin copper protein and specific antibodies of p53 were used as a capture element for p53 (wild-type and its mutants). The developed approaches allowed us to reach a detection level of p53 down to 10−17 M in both buffer and serum. The implementation of the method in a biosensor device, together with some possible developments are discussed.

Surface-enhanced Raman spectroscopy of blood plasma and serum using Ag and Au nanoparticles: a systematic study

2014 ◽  
Vol 406 (9-10) ◽  
pp. 2355-2365 ◽  
Author(s):  
Alois Bonifacio ◽  
Silvia Dalla Marta ◽  
Riccardo Spizzo ◽  
Silvia Cervo ◽  
Agostino Steffan ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Blood Plasma ◽  
Systematic Study ◽  
Au Nanoparticles ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Surface-Enhanced Raman Spectroscopy to Characterize Different Fractions of Extracellular Vesicles from Control and Prostate Cancer Patients

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 580
Author(s):  
Eric Boateng Osei ◽  
Liliia Paniushkina ◽  
Konrad Wilhelm ◽  
Jürgen Popp ◽  
Irina Nazarenko ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Raman Spectroscopy ◽  
Cancer Patients ◽  
Extracellular Vesicles ◽  
Protein Secondary Structure ◽  
Surface Enhanced Raman Spectroscopy ◽  
Cancer Diagnostics ◽  
Label Free ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Extracellular vesicles (EVs) are membrane-enclosed structures ranging in size from about 60 to 800 nm that are released by the cells into the extracellular space; they have attracted interest as easily available biomarkers for cancer diagnostics. In this study, EVs from plasma of control and prostate cancer patients were fractionated by differential centrifugation at 5000× g, 12,000× g and 120,000× g. The remaining supernatants were purified by ultrafiltration to produce EV-depleted free-circulating (fc) fractions. Spontaneous Raman and surface-enhanced Raman spectroscopy (SERS) at 785 nm excitation using silver nanoparticles (AgNPs) were employed as label-free techniques to collect fingerprint spectra and identify the fractions that best discriminate between control and cancer patients. SERS spectra from 10 µL droplets showed an enhanced Raman signature of EV-enriched fractions that were much more intense for cancer patients than controls. The Raman spectra of dehydrated pellets of EV-enriched fractions without AgNPs were dominated by spectral contributions of proteins and showed variations in S-S stretch, tryptophan and protein secondary structure bands between control and cancer fractions. We conclude that the AgNPs-mediated SERS effect strongly enhances Raman bands in EV-enriched fractions, and the fractions, EV12 and EV120 provide the best separation of cancer and control patients by Raman and SERS spectra.

Surface-enhanced Raman spectroscopy of microorganisms: limitations and applicability on the single-cell level

The Analyst ◽  
2019 ◽  
Vol 144 (3) ◽  
pp. 943-953 ◽  
Author(s):  
Ruben Weiss ◽  
Márton Palatinszky ◽  
Michael Wagner ◽  
Reinhard Niessner ◽  
Martin Elsner ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Single Cell ◽  
Environmental Studies ◽  
Surface Enhanced Raman Spectroscopy ◽  
Clinical Diagnostics ◽  
Single Cell Level ◽  
Cell Level ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Detection and characterization of microorganisms is essential for both clinical diagnostics and environmental studies.

Sign in / Sign up

Export Citation Format

Share Document