Assessment of trace of aflatoxins AFB1 and AFB2 in non-dairy beverages by molecularly imprinted polymer based micro solid-phase extraction and liquid chromatography-tandem mass spectrometry

2021 ◽  
Author(s):  
Anis Chmangui ◽  
G.D. Thilini Madurangika Jayasinghe ◽  
Mohamed Ridha Driss ◽  
Soufiane Touil ◽  
Pilar Bermejo-Barrera ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymer ◽  
Solid Phase ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Dairy Beverages

A selective molecularly imprinted polymer (MIP) adsorbent was synthesised and used in a batch micro-solid phase extraction format for isolating aflatoxins (AFB1, and AFB2) from non-dairy beverages before liquid chromatography-tandem...

Solid-phase extraction using molecularly imprinted polymer for determination of ochratoxin A in human urine

2015 ◽  
Vol 8 (1) ◽  
pp. 37-43 ◽  
Author(s):  
L. Xie ◽  
P. Sheng ◽  
W. Kong ◽  
X. Zhao ◽  
Z. Ou-Yang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Ochratoxin A ◽  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Solid Phase ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

A rapid, selective and reliable sample preparation technique employing solid-phase extraction (SPE) based on molecularly imprinted polymer (MIP) for the determination of ochratoxin A (OTA) in human urine was described. After sample adjustment to pH 2.5 with 0.1 M HCl, the urine sample was loaded onto the MIP-SPE column, and after a wash step, OTA was eluted for measurement by ultra-high performance liquid chromatography coupled with fluorescence detection. Key parameters which affected the MIP-SPE extraction efficiency were optimized as was the detection method. Under the optimised conditions, the limits of detection and quantification for OTA in urine were 0.2 ng/ml and 0.6 ng/ml, respectively. The recoveries for OTA in urine, spiked at the 0.6, 6.0 and 60 ng/ml levels, ranged from 92.0 to 98.9%. Sixty urine samples were analysed, of which four were found to contain OTA at concentrations ranging from 0.022 to 0.083 ng/ml; the positive results were confirmed by liquid chromatography coupled with tandem mass spectrometry. OTA determination in urine is a good indicator for human exposure to the mycotoxin, and this is the first report on OTA contamination in Chinese people.

Molecularly imprinted polymer-based solid phase extraction combined high performance liquid chromatography for determination of fluoroquinolones in milk

2016 ◽  
Vol 8 (27) ◽  
pp. 5511-5518 ◽  
Author(s):  
Geng Nan Wang ◽  
Kun Yang ◽  
Hui Zhi Liu ◽  
Meng Xiao Feng ◽  
Jian Ping Wang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymer ◽  
High Performance ◽  
Solid Phase ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

Molecularly imprinted polymer based solid phase extraction combined high performance liquid chromatography to determine fluoroquinolone drugs in milk.

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