Ultrasensitive homogeneous electrochemical biosensor based on CRISPR-Cas12a

2021 ◽  
Author(s):  
Jie Liu ◽  
Qing Wan ◽  
Ruijin Zeng ◽  
Dianping Tang

Taking advantage of the high-efficiency indiscriminate ssDNA cleavage activity of Cas12a in combination with the difference diffusivity of methylene blue (MB)-labeled probes (short oligonucleotide/mononucleotide) toward negatively-charged indium tin oxide (ITO)...

2016 ◽  
Vol 62 (11) ◽  
pp. 944-952 ◽  
Author(s):  
Lotfi Mhamdi ◽  
Nejib Mhamdi ◽  
Naceur Mhamdi ◽  
Philippe Lejeune ◽  
Nicole Jaffrezic ◽  
...  

This preliminary study focused on the effect of exposure to 0.5 T static magnetic fields on Escherichia coli adhesion and orientation. We investigated the difference in bacterial adhesion on the surface of glass and indium tin oxide-coated glass when exposed to a magnetic field either perpendicular or parallel to the adhesion surface (vectors of magnetic induction are perpendicular or parallel to the adhesion surface, respectively). Control cultures were simultaneously grown under identical conditions but without exposure to the magnetic field. We observed a decrease in cell adhesion after exposure to the magnetic field. Orientation of bacteria cells was affected after exposure to a parallel magnetic field. On the other hand, no effect on the orientation of bacteria cells was observed after exposure to a perpendicular magnetic field.


1989 ◽  
Vol 54 (26) ◽  
pp. 2674-2676 ◽  
Author(s):  
X. Li ◽  
M. W. Wanlass ◽  
T. A. Gessert ◽  
K. A. Emery ◽  
T. J. Coutts

Catalysts ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 848
Author(s):  
Cheng Gong ◽  
Shiyin Xu ◽  
Peng Xiao ◽  
Feifan Liu ◽  
Yunhui Xu ◽  
...  

A hybrid material of indium tin-oxide (ITO) wrapped titanium dioxide and reduced graphene oxide (ITO-rGO and TiO2) was prepared using a facile hydrothermal technique. TiO2 nanorods were in situ grown on the surface of rGO (rGO and TiO2), and which was then assembled onto ITO substrate layer by layer with formation of a 3D structure. ITO-rGO and TiO2 exhibit low charge transfer resistance at the electrode-electrolyte interface and have good photoresponsive ability. Methylene blue (MB) can be effectively adsorbed and enriched onto ITO-rGO and TiO2 surface. The adsorption kinetics and thermodynamics of ITO-rGO and TiO2 were evaluated, showing that the exothermic and entropy-driven reaction were the main thermodynamic processes, and the Langmuir isotherm was the ideal model for adsorption fitting. Meanwhile, ITO greatly improved degradation of rGO and TiO2 because electrons can be collected by ITO before recombination and MB can easily enter into the 3D structure of rGO and TiO2. The highest photodegradation rate of MB reached 93.40% for ITO-rGO and TiO2 at pH 9. Additionally, ITO-rGO and TiO2 successfully solved the problems of being difficult to recycle and causing secondary pollution of traditional TiO2 catalysts. Therefore, ITO-rGO and TiO2 may be a potential photocatalyst for degrading organic pollutants in water.


2008 ◽  
Vol 8 (3) ◽  
pp. 1341-1345
Author(s):  
Sha Xiong ◽  
Shihua Huang ◽  
Aiwei Tang ◽  
Feng Teng

Electroluminescence was obtained from an indium-tin-oxide/poly[2-methoxy-5-(2′-ethylhexyloxy)-1,4-phenylene vinylene] (MEH-PPV): ZnSe/2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline (BCP)/8-tris-hydroxyquinoline (Alq3)/LiF/Al structured device, in which ZnSe nanocrystals were synthesized in aqueous solution by using mercapto-acetate acid as stabilizer. The mechanical, electrical, and optical properties of the device were established. The photoluminescence and electroluminescence spectra changed with the mass ratio of ZnSe to MEH-PPV in the composite. Comparison between the absorption spectra and photoluminescence spectra of the ZnSe nanocrystals and the MEH-PPV thin film exhibited an effective energy transfer from ZnSe nanocrystals to MEH-PPV, which was one reason for the difference between the photoluminescence and electroluminescence spectra of the MEH-PPV: ZnSe composite film. The recombination mechanism of ZnSe nanocrystals under photo excitation and electric injection was investigated with the help of a single layer device structure of indium-tin-oxide/ZnSe/LiF/Al.


1987 ◽  
Vol 23 (10) ◽  
pp. 527-528 ◽  
Author(s):  
D.G. Parker ◽  
P.G. Say ◽  
A.M. Hansom ◽  
W. Sibbett

1998 ◽  
Vol 37 (Part 2, No. 7B) ◽  
pp. L879-L881 ◽  
Author(s):  
Takahisa Omata ◽  
Hiroyuki Fujiwara ◽  
Shinya Otsuka ◽  
Yao ◽  
Matsuo ◽  
...  

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