Ultrasensitive detection of specific IgE based on nanomagnetic capture and separation with AuNP-anti-IgE nanobioprobe for signal amplification

2021 ◽  
Author(s):  
Mengda Cao ◽  
Yongxin Liu ◽  
Chen Lu ◽  
Miao Guo ◽  
Lin Li ◽  
...  
Keyword(s):  
Allergic Disease ◽  
Signal Amplification ◽  
Specific Ige ◽  
Ultrasensitive Detection ◽  
Accurate Detection ◽  
Personalized Diagnosis

The accurate detection of allergen specific IgE (sIgE) is fundamental in the diagnosis of allergic disease. The present commercial platforms fail to meet the need for personalized diagnosis, due to...

A novel label free long non-coding RNA electrochemical biosensor based on green l-cysteine electrodeposition and Au–Rh hollow nanospheres as tags

RSC Advances ◽  
2015 ◽  
Vol 5 (64) ◽  
pp. 51990-51999 ◽  
Author(s):  
Fei Liu ◽  
Guiming Xiang ◽  
Liqun Zhang ◽  
Dongneng Jiang ◽  
Linlin Liu ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Single Wall Carbon Nanotubes ◽  
Complex Signal ◽  
Ultrasensitive Detection ◽  
Label Free ◽  
Hollow Nanospheres ◽  
Non Coding Rna ◽  
Long Non Coding Rna

lncRNA biosensor based on single-wall carbon nanotubes wrapped with Au–Rh hollow nanospheres (Au/Rh-HNP@SWCNT) complex signal amplification and an l-Cys Au nano-film provided ultrasensitive detection for the nuclear paraspeckle assembly transcript 1 (NEAT1).

scholarly journals Invited review: The role of allergen‐specific IgE, IgG and IgA in allergic disease

Allergy ◽  
2021 ◽  
Author(s):  
Mohamed H. Shamji ◽  
Rudolf Valenta ◽  
Theodore Jardetzky ◽  
Valerie Verhasselt ◽  
Stephen R. Durham ◽  
...  
Keyword(s):  
Allergic Disease ◽  
Specific Ige

A new enzyme-free quadratic SERS signal amplification approach for circulating microRNA detection in human serum

2015 ◽  
Vol 51 (90) ◽  
pp. 16271-16274 ◽  
Author(s):  
Jing Zheng ◽  
Dandan Ma ◽  
Muling Shi ◽  
Junhui Bai ◽  
Yinhui Li ◽  
...  
Keyword(s):  
Human Serum ◽  
Signal Amplification ◽  
Circulating Microrna ◽  
Ultrasensitive Detection ◽  
Circulating Mirna ◽  
Sers Signal ◽  
Microrna Detection

In this communication, we proposed a new enzyme-free quadratic SERS signal amplification approach for the ultrasensitive detection of circulating miRNA in human serum.

A disposable paper-based electrochemiluminescence device for ultrasensitive monitoring of CEA based on Ru(bpy)32+@Au nanocages

RSC Advances ◽  
2015 ◽  
Vol 5 (36) ◽  
pp. 28324-28331 ◽  
Author(s):  
Chaomin Gao ◽  
Min Su ◽  
Yanhu Wang ◽  
Shenguang Ge ◽  
Jinghua Yu
Keyword(s):  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Working Electrode

Ultrasensitive detection of CEA based on Ag nanospheres modified paper working electrode platform and Ru(bpy)32+@Au nanocages signal amplification.

Proficiency Survey-Based Evaluation of Clinical Total and Allergen-Specific IgE Assay Performance

2010 ◽  
Vol 134 (7) ◽  
pp. 975-982 ◽  
Author(s):  
Robert G. Hamilton
Keyword(s):  
Allergic Disease ◽  
Immunoglobulin E ◽  
Diagnostic Algorithm ◽  
Specific Ige ◽  
Total Serum ◽  
Design Data ◽  
Ige Antibody ◽  
Marked Variability ◽  
Specific Immunoglobulin E ◽  
History Of

Abstract Context.—The diagnostic algorithm for human allergic disease involves confirmation of sensitization by detection of allergen-specific immunoglobulin E (IgE) antibody in individuals suspected of having allergic disease because of a history of allergic symptoms after known allergen exposure. Previous studies showed wide disparity among clinically reported allergen-specific IgE levels from different serologic assays. Objective.—To validate the relative analytic performance (sensitivity, interassay reproducibility, linearity/parallelism, intermethod agreement) of clinically used total and allergen-specific IgE assays by using College of American Pathologists' Diagnostic Allergy “SE” Proficiency Survey data. Design.—Data from 2 SE survey cycles were used to assess relative analytic performance of the ImmunoCAP (Phadia), Immulite (Siemens Healthcare-Diagnostics), and HYTEC 288 (HYCOR-Agilent Technologies) total and allergen-specific IgE assays. In each cycle, 2 recalcified plasma pools from atopic donors were diluted twice with IgE-negative serum and evaluated in approximately 200 federally certified clinical laboratories for total IgE and IgE antibody to 5 allergen specificities. Statistical analysis evaluated analytic sensitivity, linearity, reproducibility, and intermethod agreement. Results.—Interlaboratory intramethod, intermethod, and interdilution agreement of all 6 clinically used total serum IgE assays were excellent, with coefficients of variation (CVs) below 15%. Interlaboratory intramethod, and interdilution agreement of 3 clinically used allergen-specific IgE assays were also excellent with CVs below 15%. However, intermethod CVs identified between-assay disagreement greater than 20% in 80% of allergen-specific IgE measurements. Allergen reagents and patients' immune response heterogeneity are suggested probable causes. Conclusions.—Clinical total and allergen-specific IgE assays display excellent analytic sensitivity, precision, reproducibility, and linearity. Marked variability in quantitative estimates of allergen-specific IgE from clinically used automated immunoassays is a concern that may be ameliorated with component allergen use.

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