Quantitative trace metal determinations in cell culture media using LS-APGD-MS and ICP-OES with free/bound species differentiation following polymer fiber separations

2021 ◽  
Vol 13 (16) ◽  
pp. 1945-1954
Author(s):  
Katja A. Hall ◽  
Htoo W. Paing ◽  
R. Kenneth Marcus
Keyword(s):  
Cell Culture ◽  
Trace Metal ◽  
Culture Media ◽  
Media Analysis ◽  
Species Differentiation ◽  
Polymer Fiber ◽  
Icp Oes ◽  
Cell Culture Media

The use of LS-APGD-MS is demonstrated for applications in cell culture media analysis and validated by ICP-OES. Use of simple polymer fiber injector loop allows determinations of the relative free vs. bound fractions of the metals.

scholarly journals Degradation of silicon photonic biosensors in cell culture media: analysis and prevention

2017 ◽  
Vol 8 (6) ◽  
pp. 2924
Author(s):  
Graham J. Triggs ◽  
Gareth J. O. Evans ◽  
Thomas F. Krauss
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Media Analysis ◽  
Cell Culture Media ◽  
Silicon Photonic ◽  
Photonic Biosensors

scholarly journals Evaluation of MDA-MB-468 Cell Culture Media Analysis in Predicting Triple-Negative Breast Cancer Patient Sera Metabolic Profiles

Metabolites ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 173
Author(s):  
Wojciech Wojtowicz ◽  
Anna Wróbel ◽  
Karolina Pyziak ◽  
Radosław Tarkowski ◽  
Alicja Balcerzak ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Culture Media ◽  
Media Analysis ◽  
Serum Samples ◽  
Cell Culture Media ◽  
Time Courses ◽  
And Control

Triple-negative breast cancer (TNBC) is characterized by limited survival, poor prognosis, and high recurrence. Understanding the metabolic adaptations of TNBC could help reveal improved treatment regiments. Here we performed a comprehensive 1H NMR metabolic characterization of the MDA-MB-468 cell line, a commonly used model of TNBC, followed by an analysis of serum samples obtained from TNBC patients and healthy controls. MDA-MB-468 cells were cultured, and changes in the metabolic composition of the medium were monitored for 72 h. Based on time courses, metabolites were categorized as being consumed, being produced, or showing a mixed behavior. When comparing TNBC and control samples (HC), and by using multivariate and univariate analyses, we identified nine metabolites with differing profiles). The serum of TNBC patients was characterized by higher levels of glucose, glutamine, citrate, and acetoacetate and by lower levels of lactate, alanine, tyrosine, glutamate, and acetone. A comparative analysis between MDA-MB-468 cell culture media and TNBC patients’ serum identified a potential systemic response to the carcinogenesis-associated processes, highlighting that MDA-MB-468 cells footprint does not reflect metabolic changes observed in studied TNBC serum fingerprint.

Cell culture media: NMR approaches to evaluating quality and nutrient consumption

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
KB Killday ◽  
AS Freund ◽  
C Fischer ◽  
KL Colson
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Cell Culture Media ◽  
Nutrient Consumption

Selection and preliminary application of DNA aptamer targeting A549 excreta in cell culture media

2021 ◽  
pp. 106811
Author(s):  
Yuanbin Guo ◽  
Ming Shi ◽  
Xiujuan Liu ◽  
Huagang Liang ◽  
Liming Gao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Dna Aptamer ◽  
Cell Culture Media ◽  
Preliminary Application

scholarly journals Benchmarking of commercially available CHO cell culture media for antibody production

2015 ◽  
Vol 99 (11) ◽  
pp. 4645-4657 ◽  
Author(s):  
David Reinhart ◽  
Lukas Damjanovic ◽  
Christian Kaisermayer ◽  
Renate Kunert
Keyword(s):  
Cell Culture ◽  
Antibody Production ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Cho Cell Culture
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