Fiber bundle based chemiluminescence array detection

2021 ◽  
Author(s):  
Gen Chen ◽  
Tan He ◽  
Song Sun ◽  
Jun Bao ◽  
Hua Cui ◽  
...  
Keyword(s):  
Clinical Diagnosis ◽  
Fiber Bundle ◽  
Simultaneous Detection ◽  
Multiplex Analysis ◽  
Array Detection ◽  
Increasing Demand ◽  
Multiple Samples

Chemiluminescence (CL) is a dominated technology in clinical diagnosis. In order to meet increasing demand for sensitive and simultaneous detection of chemiluminescence from multiple samples, the development of multiplex analysis...

scholarly journals Simultaneous detection of antibodies to fiveActinobacillus pleuropneumoniaeserovars using bead-based multiplex analysis

2017 ◽  
Vol 29 (6) ◽  
pp. 797-804 ◽  
Author(s):  
Sanne Schou Berger ◽  
Klara Tølbøll Lauritsen ◽  
Ulrik Boas ◽  
Peter Lind ◽  
Lars Ole Andresen
Keyword(s):  
Simultaneous Detection ◽  
Multiplex Analysis

Simultaneous detection of porcine cytokines by multiplex analysis: Development of magnetic bioplex assay

2009 ◽  
Vol 130 (1-2) ◽  
pp. 53-58 ◽  
Author(s):  
Mette Bjerre ◽  
Troels Krarup Hansen ◽  
Allan Flyvbjerg ◽  
Else Tønnesen
Keyword(s):  
Simultaneous Detection ◽  
Multiplex Analysis

scholarly journals Application of Real-Time Simultaneous Amplification and Testing Method to Accurately and Rapidly Detect Extra-pulmonary Tuberculosis

2020 ◽  
Author(s):  
Tongxin Li ◽  
Tao Shi ◽  
Ying Sun ◽  
Kan Zhou ◽  
Zhenggu Huang ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Clinical Diagnosis ◽  
Sensitivity And Specificity ◽  
Simultaneous Detection ◽  
Culture Method ◽  
Culture Methods ◽  
Predictive Values ◽  
Extra Pulmonary Tuberculosis ◽  
Testing Method ◽  
Extra Pulmonary

Abstract Background: This study aimed to establish and evaluate a simultaneous amplification and testing method for detection of extra-pulmonary tuberculosis (EPTB). Methods: From January 2016 and December 2017 the pus or surgical excision from the lesions of inpatients admitted from Chongqing Public Health Treatment Center were collected. According to the clinical diagnosis, the samples were divided into two groups including EPTB (Group A) and other diseases excluded from tuberculosis diseases (Group B). Simultaneous detection of Mycobacterium tuberculosis (MTB) used Roche culture method, liquid culture method and simultaneous amplification and testing (SAT) method. The sensitivity and specificity of the SAT method were compared with culture methods and clinical diagnosis of EPTB. Results: For 433 EPTB specimens and 49 non-TB specimens, the simultaneous amplification and testing tuberculosis (SAT-TB) results correlated with 80.5% (388/482 specimens) of the culture assay results. The sensitivity, specificity, and positive and negative predictive values of the SAT-TB test for the diagnosis of EPTB were 83.6%, 79.4%, 59.4%, and 93.0%, respectively, compared to culture methods. Compared with the clinical diagnosis of patients, the sensitivity and specificity of the SAT-TB test were 41.6% and 100%, respectively, the cultures test were 29.3% and 98.0%. Conclusions: SAT test is a simple and rapid test with high specificity which may enhance the detection of EPTB. SAT-TB is a higher clinical diagnosis value for EPTB in clinical microbiology laboratories.

scholarly journals Application of Real-Time Simultaneous Amplification and Testing Method to Accurately and Rapidly Detect Extra-pulmonary Tuberculosis

2020 ◽  
Author(s):  
Tongxin Li ◽  
Tao Shi ◽  
Ying Sun ◽  
Kan Zhou ◽  
Zhenggu Huang ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Clinical Diagnosis ◽  
Sensitivity And Specificity ◽  
Simultaneous Detection ◽  
Culture Method ◽  
Culture Methods ◽  
Predictive Values ◽  
Extra Pulmonary Tuberculosis ◽  
Testing Method ◽  
Extra Pulmonary

Abstract Background: This study aimed to establish and evaluate a simultaneous amplification and testing method for detection of extra-pulmonary tuberculosis. Methods: From January 2016 and December 2017 the pus or surgical excision from the lesions of inpatients admitted from Chongqing Public Health Treatment Center were collected. According to the clinical diagnosis, the samples were divided into two groups including extra-pulmonary tuberculosis (Group A) and other diseases excluded from tuberculosis diseases (Group B). Simultaneous detection of Mycobacterium tuberculosis (MTB) used Roche culture method, liquid culture method and simultaneous amplification and testing method (SAT). The sensitivity and specificity of the SAT method were compared with culture methods and clinical diagnosis of extra-pulmonary tuberculosis. Results: For 433 extra-pulmonary tuberculosis (EPTB) specimens and 49 non-TB specimens, the SAT-TB results correlated with 80.5% (388/482 specimens) of the culture assay results. The sensitivity, specificity, and positive and negative predictive values of the SAT-TB test for the diagnosis of EPTB were 83.6%, 79.4%, 59.4%, and 93.0%, respectively, compared to culture methods. Compared with the clinical diagnosis of patients, the sensitivity and specificity of the SAT-TB test were 41.6% and 100%, respectively, the cultures test were 29.3% and 98.0%. Conclusions: SAT test is a simple and rapid test with high specificity which may enhance the detection of EPTB. SAT-TB is a higher clinical diagnosis value for EPTB in clinical microbiology laboratories.

scholarly journals Application of Real-Time Simultaneous Amplification and Testing Method to Accurately and Rapidly Detect Extra-pulmonary Tuberculosis

2020 ◽  
Author(s):  
Tongxin Li ◽  
Tao Shi ◽  
Ying Sun ◽  
Kan Zhou ◽  
Zhenggu Huang ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Clinical Diagnosis ◽  
Sensitivity And Specificity ◽  
Simultaneous Detection ◽  
Culture Method ◽  
Culture Methods ◽  
Predictive Values ◽  
Extra Pulmonary Tuberculosis ◽  
Testing Method ◽  
Extra Pulmonary

Abstract Background: This study aimed to establish and evaluate a simultaneous amplification and testing method for detection of extra-pulmonary tuberculosis. Methods: From January 2016 and December 2017 the pus or surgical excision from the lesions of inpatients admitted from Chongqing Public Health Treatment Center were collected. According to the clinical diagnosis, the samples were divided into two groups including extra-pulmonary tuberculosis (Group A) and other diseases excluded from tuberculosis diseases (Group B). Simultaneous detection of Mycobacterium tuberculosis (MTB) used Roche culture method, liquid culture method and simultaneous amplification and testing method (SAT). The sensitivity and specificity of the SAT method were compared with culture methods and clinical diagnosis of extra-pulmonary tuberculosis. Results: For 433 extra-pulmonary tuberculosis (EPTB) specimens and 49 non-TB specimens, the SAT-TB results correlated with 80.5% (388/482 specimens) of the culture assay results. The sensitivity, specificity, and positive and negative predictive values of the SAT-TB test for the diagnosis of EPTB were 83.6%, 79.4%, 59.4%, and 93.0%, respectively, compared to culture methods. Compared with the clinical diagnosis of patients, the sensitivity and specificity of the SAT-TB test were 41.6% and 100%, respectively, the cultures test were 29.3% and 98.0%. Conclusions: SAT test is a simple and rapid test with high specificity which may enhance the detection of EPTB. SAT-TB is a higher clinical diagnosis value for EPTB in clinical microbiology laboratories.

scholarly journals MuClone: Somatic mutation detection and classification through probabilistic integration of clonal population structure

2017 ◽  
Author(s):  
Fatemeh Dorri ◽  
Sean Jewell ◽  
Alexandre Bouchard-Côté ◽  
Sohrab P. Shah
Keyword(s):  
Mutation Detection ◽  
Simultaneous Detection ◽  
Whole Genome Sequence ◽  
Whole Genome ◽  
Sequencing Data ◽  
Single Nucleotide Variants ◽  
Clonal Population ◽  
Low Prevalence ◽  
Multiple Samples

AbstractAccurate detection and classification of somatic single nucleotide variants (SNVs) is important in defining the clonal composition of human cancers. Existing tools are prone to miss low prevalence mutations and methods for classification of mutations into clonal groups across the whole genome are underdeveloped. Increasing interest in deciphering clonal population dynamics over multiple samples in time or anatomic space from the same patient is resulting in whole genome sequence (WGS) data from phylogenetically related samples. With the access to this data, we posited that injecting clonal structure information into the inference of mutations from multiple samples would improve mutation detection.We developed MuClone: a novel statistical framework for simultaneous detection and classification of mutations across multiple tumour samples of a patient from whole genome or exome sequencing data. The key advance lies in incorporating prior knowledge about the cellular prevalences of clones to improve the performance of detecting mutations, particularly low prevalence mutations. We evaluated MuClone through synthetic and real data from spatially sampled ovarian cancers. Results support the hypothesis that clonal information improves sensitivity in detecting somatic mutations without compromising specificity. In addition, MuClone classifies mutations across whole genomes of multiple samples into biologically meaningful groups, providing additional phylogenetic insights and enhancing the study of WGS-derived clonal dynamics.

scholarly journals On the Use of Infrared Thermography for the Estimation of Melting Enthalpy

2021 ◽  
Vol 11 (13) ◽  
pp. 5915
Author(s):  
Clément Mailhé ◽  
Marie Duquesne ◽  
Elena Palomo del Barrio ◽  
Alexandre Godin
Keyword(s):  
Thermal Analysis ◽  
Infrared Thermography ◽  
Simultaneous Detection ◽  
Melting Enthalpy ◽  
Scanning Calorimetry ◽  
Eutectic Mixtures ◽  
Working Principle ◽  
The One ◽  
Multiple Samples ◽  
Material Screening

A calorimetry method based on infrared thermography is showing promise for material screening, allowing the simultaneous detection of phase transitions of multiple samples at a time, hence enabling the establishment of phase diagrams in a record time. The working principle of this method is similar to the one of Differential Thermal Analysis. Therefore, this work aims at identifying if the melting enthalpy of materials could be estimated on the same basis using infrared thermography. In this work, the melting of six eutectic mixtures of fatty acids is estimated under three considerations. The results are compared to Differential Scanning Calorimetry measurements and literature data. The accuracy of the method is discussed and improvements are proposed.

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