A new urinary exosome enrichment method by a combination of ultrafiltration and TiO2 nanoparticles

2021 ◽  
Vol 13 (13) ◽  
pp. 1591-1600
Author(s):  
Xiaochao Xiang ◽  
Fulin Guan ◽  
Fenglong Jiao ◽  
Hang Li ◽  
Wanjun Zhang ◽  
...  
Keyword(s):  
Tio2 Nanoparticles ◽  
Human Urine ◽  
High Purity ◽  
Enrichment Method ◽  
New Strategy ◽  
Urinary Exosome

The workflow of separation and enrichment of exosomes by ultrafiltration–TiO2. We proposed a new strategy for facile exosome isolation from human urine by utilizing the ultrafiltration technique and TiO2, which can significantly reduce urine volumes, increase exposure of the material to exosomes in urine and obtain high purity exosomes.

Novel method for rapid enrichment of high purity circulating tumor cells (CTCs) for prostate cancer (PCa) gene expression profiling.

2018 ◽  
Vol 36 (6_suppl) ◽  
pp. 271-271
Author(s):  
Gareth Morrison ◽  
Nita Jojo ◽  
Alexander Cunha ◽  
Yucheng Xu ◽  
Peggy S. Robinson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Gene Expression Profiling ◽  
High Purity ◽  
Whole Blood ◽  
Expression Profiling ◽  
Cell Recovery ◽  
Enrichment Method ◽  
Qrt Pcr ◽  
Cd45 Depletion

271 Background: CTC RNA analysis currently involves single cell recovery that is laborious and expensive, or alternatively lysis of preserved whole blood which yields RNA predominantly from leukocytes which vastly outnumber CTCs. To effectively characterize gene expression in large patient cohorts, new enrichment methodologies are needed that yield high purity CTC populations while preserving RNA integrity. Here we describe a simple yet robust method for enrichment of prostate CTCs for gene expression analysis. Methods: Blood was drawn with informed consent under an IRB-approved protocol. For initial optimization, CFSE-stained PCa cells were spiked into healthy blood and recovered using various combinations of 2 methods: microfluidic enrichment (Parsortix™ system) and CD45 depletion. For assay qualification, a prostate-specific multiplexed qRT-PCR gene expression panel was developed. Enrichment and gene expression were tested initially using PCa cell lines spiked into healthy blood, then metastatic castrate resistant prostate cancer (mCRPC) blood samples in parallel with CellSearch enumeration. Results: Optimal enrichment of live cells was achieved with CD45 depletion followed by microfluidic enrichment, resulting in an average spiked cell recovery of 30% and approximately 100 contaminating background leukocytes. Using this enrichment method, prostate specific genes were detectable by multiplexed qRT-PCR down to 25 cells spiked into 7.5 ml whole blood, and transcripts were not measurable in matched healthy blood controls. When applied to mCRPC patient blood containing CTCs by CellSearch, multiplexed qRT-PCR successfully detected prostate specific genes in all samples. Conclusions: We developed a novel enrichment method capable of rapidly and efficiently recovering live CTCs with high purity, free of magnetic beads and with very few background leukocytes. Captured cells yielded high-quality RNA with high sensitivity and specificity for prostate-specific transcripts. This approach is applicable to high throughput gene expression profiling assays and offers an alternative to laborious single cell recovery or non-cancer-specific whole blood fixation.

TiO2 nanoparticles vs. TiO2 nanowires as support in hydrogen peroxide direct synthesis: the influence of N and Au doping

RSC Advances ◽  
2016 ◽  
Vol 6 (105) ◽  
pp. 103311-103319 ◽  
Author(s):  
Nicola Gemo ◽  
Federica Menegazzo ◽  
Pierdomenico Biasi ◽  
Anjana Sarkar ◽  
Ajaikumar Samikannu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Tio2 Nanoparticles ◽  
Nitrogen Doping ◽  
Direct Synthesis ◽  
Tio2 Nanowires ◽  
New Strategy

Nitrogen doping is a new strategy to improve catalysts for H2O2 direct synthesis.

scholarly journals Photocatalytic Hydrogen Production from Urine Using Sr-Doped TiO2 Photocatalyst with Subsequent Phosphorus Recovery via Struvite Crystallization

Catalysts ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1012
Author(s):  
Oranoot Sittipunsakda ◽  
Patiya Kemacheevakul ◽  
Navadol Laosiripojana ◽  
Surawut Chuangchote
Keyword(s):  
Hydrogen Production ◽  
Tio2 Nanoparticles ◽  
Human Urine ◽  
Environmental Problems ◽  
Phosphorus Recovery ◽  
Impregnation Method ◽  
Doped Tio2 ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Struvite Crystallization

Currently, the discharge of wastewater and utilization of phosphorus (P) in human activities cause some environmental problems, such as high organic pollutants in aquatic environments which results in dirty water sources, and a shortage of phosphate rock reserves due to the high demand of P. Therefore, fuel energy and struvite crystallization from waste sources can be considered interesting alternatives. In this work, the modified catalyst for hydrogen production, along with solving environmental problems, was examined. The strontium (Sr) doped-titanium dioxide (TiO2) nanoparticles were synthesized by wetness impregnation method. The synthesized catalyst was characterized using UV-vis spectroscopy (UV-vis), photoluminescence (PL), X-ray diffraction (XRD), photoluminescence (PL), and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS). The Sr-doped TiO2 catalysts had been utilized as the photocatalyst for the hydrogen production from synthetic human urine (a representative of waste source). The doping content of Sr in TiO2 varied from 0.5, 1, 2, and 4%, and the photocatalytic performances were compared with pristine TiO2 nanoparticles. The results showed that 1% Sr-doped TiO2 had the highest photocatalytic activity for hydrogen production and decreased the amount of chemical oxygen demand (COD) in the synthetic human urine. Subsequently, P could be recovered from the treated human urine in the form of struvite.

A New Strategy of Integrated Control and On-line Optimization on High-purity Distillation Process

2010 ◽  
Vol 18 (1) ◽  
pp. 66-79 ◽  
Author(s):  
Wenxiang LÜ ◽  
Ying ZHU ◽  
Dexian HUANG ◽  
Yongheng JIANG ◽  
Yihui JIN
Keyword(s):  
High Purity ◽  
Integrated Control ◽  
Distillation Process ◽  
New Strategy ◽  
On Line

Sustainable Chemical Synthesis for Phosphorus-Doping of TiO2 Nanoparticles by Upcycling Human Urine and Impact of Doping on Energy Applications

2017 ◽  
Vol 5 (3) ◽  
pp. 2393-2399 ◽  
Author(s):  
Sreejith P. Madhusudanan ◽  
Binitha Gangaja ◽  
Anjusree G. Shyla ◽  
A. Sreekumaran Nair ◽  
Shantikumar V. Nair ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
Tio2 Nanoparticles ◽  
Human Urine ◽  
Phosphorus Doping ◽  
Energy Applications

Decreasing the oxidative potential of TiO2 nanoparticles through modification of the surface with carbon: a new strategy for the production of safe UV filters

2010 ◽  
Vol 46 (44) ◽  
pp. 8478 ◽  
Author(s):  
Stefano Livraghi ◽  
Ingrid Corazzari ◽  
Maria Cristina Paganini ◽  
Giacomo Ceccone ◽  
Elio Giamello ◽  
...  
Keyword(s):  
Tio2 Nanoparticles ◽  
Uv Filters ◽  
Oxidative Potential ◽  
New Strategy ◽  
Modification Of The Surface
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