scholarly journals Correction: Synchrotron macro ATR-FTIR microspectroscopy for high-resolution chemical mapping of single cells

The Analyst ◽  
2021 ◽  
Author(s):  
Jitraporn Vongsvivut ◽  
David Pérez-Guaita ◽  
Bayden R. Wood ◽  
Philip Heraud ◽  
Karina Khambatta ◽  
...  
Keyword(s):  
High Resolution ◽  
Single Cells ◽  
Chemical Mapping ◽  
Ftir Microspectroscopy

Correction for ‘Synchrotron macro ATR-FTIR microspectroscopy for high-resolution chemical mapping of single cells’ by Jitraporn Vongsvivut et al., Analyst, 2019, 144, 3226–3238, DOI: 10.1039/C8AN01543K.

scholarly journals Synchrotron macro ATR-FTIR microspectroscopy for high-resolution chemical mapping of single cells

The Analyst ◽  
2019 ◽  
Vol 144 (10) ◽  
pp. 3226-3238 ◽  
Author(s):  
Jitraporn Vongsvivut ◽  
David Pérez-Guaita ◽  
Bayden R. Wood ◽  
Philip Heraud ◽  
Karina Khambatta ◽  
...  
Keyword(s):  
High Resolution ◽  
Single Cell ◽  
Spatial Resolution ◽  
Environmental Science ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Cell Analysis ◽  
Chemical Mapping ◽  
Ftir Microspectroscopy ◽  
Resolution Single

Coupling synchrotron IR beam to an ATR element enhances spatial resolution suited for high-resolution single cell analysis in biology, medicine and environmental science.

scholarly journals Synchrotron macro ATR-FTIR microspectroscopy for high-resolution chemical mapping of single cells

2020 ◽  
Author(s):  
Vongsvivut Jitraporn (Pimm) ◽  
Pérez-Guaita D. ◽  
Wood B. R. ◽  
Heraud P. ◽  
Khambatta K ◽  
...  
Keyword(s):  
High Resolution ◽  
Single Cells ◽  
Chemical Mapping ◽  
Ftir Microspectroscopy

scholarly journals High resolution surface plasmon resonance imaging for single cells

2014 ◽  
Vol 15 (1) ◽  
Author(s):  
Alexander W Peterson ◽  
Michael Halter ◽  
Alessandro Tona ◽  
Anne L Plant
Keyword(s):  
Surface Plasmon Resonance ◽  
High Resolution ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Single Cells ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging

scholarly journals μCB-seq: Microfluidic cell barcoding and sequencing for high-resolution imaging and sequencing of single cells

2020 ◽  
Author(s):  
Tyler N. Chen ◽  
Anushka Gupta ◽  
Mansi Zalavadia ◽  
Aaron M. Streets
Keyword(s):  
High Resolution ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Protein Localization ◽  
Single Cells ◽  
Optical Measurements ◽  
Detection Sensitivity ◽  
High Resolution Imaging ◽  
Cell Analysis ◽  
Resolution Imaging

AbstractSingle-cell RNA sequencing (scRNA-seq) enables the investigation of complex biological processes in multicellular organisms with high resolution. However, many phenotypic features that are critical to understanding the functional role of cells in a heterogeneous tissue or organ are not directly encoded in the genome and therefore cannot be profiled with scRNA-seq. Quantitative optical microscopy has long been a powerful approach for characterizing diverse cellular phenotypes including cell morphology, protein localization, and chemical composition. Combining scRNA-seq with optical imaging has the potential to provide comprehensive single-cell analysis, allowing for functional integration of gene expression profiling and cell-state characterization. However, it is difficult to track single cells through both measurements; therefore, coupling current scRNA-seq protocols with optical measurements remains a challenge. Here, we report Microfluidic Cell Barcoding and Sequencing (μCB-seq), a microfluidic platform that combines high-resolution imaging and sequencing of single cells. μCB-seq is enabled by a novel fabrication method that preloads primers with known barcode sequences inside addressable reaction chambers of a microfluidic device. In addition to enabling multi-modal single-cell analysis, μCB-seq improves gene detection sensitivity, providing a scalable and accurate method for information-rich characterization of single cells.

Monitoring UVR induced damage in single cells and isolated nuclei using SR-FTIR microspectroscopy and 3D confocal Raman imaging

The Analyst ◽  
2014 ◽  
Vol 139 (17) ◽  
pp. 4200-4209 ◽  
Author(s):  
Ewelina Lipiec ◽  
Keith R. Bambery ◽  
Philip Heraud ◽  
Wojciech M. Kwiatek ◽  
Don McNaughton ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Lipid Accumulation ◽  
Single Cells ◽  
Raman Imaging ◽  
Isolated Nuclei ◽  
Ftir Microspectroscopy ◽  
Confocal Raman ◽  
Ftir And Raman Spectroscopy

Melanocytes exposed to artificial sunlight and analysed with FTIR and Raman spectroscopy show changes in DNA bands and evidence of lipid accumulation.

scholarly journals High resolution chemical mapping of biomimetic membranes by force volume imaging

2008 ◽  
Author(s):  
Matthew R. Nussio ◽  
Nicolas H. Voelcker ◽  
Matthew J. Sykes ◽  
Ben S. Flavel ◽  
John O. Miners ◽  
...  
Keyword(s):  
High Resolution ◽  
Chemical Mapping ◽  
Biomimetic Membranes ◽  
Volume Imaging ◽  
Force Volume

Near-field chemical mapping of gold nanostructures using a functionalized scanning probe

2017 ◽  
Vol 19 (46) ◽  
pp. 31063-31071 ◽  
Author(s):  
C. Dab ◽  
C. Awada ◽  
A. Merlen ◽  
A. Ruediger
Keyword(s):  
Raman Spectroscopy ◽  
Surface Plasmon Resonance ◽  
High Resolution ◽  
Surface Plasmon ◽  
Photophysical Properties ◽  
Near Field ◽  
Scanning Probe ◽  
Gold Nanostructures ◽  
Chemical Mapping ◽  
Tip Enhanced Raman Spectroscopy

We report on photochemical and photophysical properties produced by Surface Plasmon Resonance (SPR) on metallic nanograins by means of high resolution Functionalized Tip-Enhanced Raman Spectroscopy (F-TERS).

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