Effects of amino acid additives on protein solubility - insights from desorption and direct electrospray ionization mass spectrometry

The Analyst ◽  
2021 ◽  
Author(s):  
Roshan Javanshad ◽  
Andre R. Venter
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Amino Acid ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Signal Intensity ◽  
Protein Solubility ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Naturally Occurring

Naturally occurring amino acids have been broadly used as additives to improve protein solubility and inhibit aggregation. In this study, improvements in protein signal intensity obtained with the addition of...

Identification of complexes involving toxic heavy metals with amino acid ligands by electrospray ionization mass spectrometry

2004 ◽  
Vol 82 (8) ◽  
pp. 1254-1259 ◽  
Author(s):  
Neil Burford ◽  
Melanie D Eelman ◽  
Wesley G LeBlanc
Keyword(s):  
Mass Spectrometry ◽  
Heavy Metals ◽  
Amino Acids ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Toxic Heavy Metals ◽  
Esi Ms ◽  
Naturally Occurring

Electrospray ionization mass spectra (ESI-MS) of reaction mixtures containing ions of the heavy metals, cadmium, mercury, thallium, lead, and bismuth with each of the naturally occurring amino acids allows for definitive identification of monocationic complexes in the gas phase. Prominent m/z peaks are assigned to ions of the type [E(Am) – xH]+ (x = 0, 1, 2), [E(Am)2 – xH]+ (x = 0, 1, 2), [E2(Am) – xH]+ (x = 1, 2, 3), and (or) [E2(Am)2 – xH]+ (x = 1, 3, 5) for all amino acids studied (Am) with all metals studied (E = Cd, Hg, Tl, Pb) except bismuth, which shows complexes with only seven amino acids (Am = His, Thr, Met, Cys, Hcys, Asn, Gln). The results demonstrate the potential application of ESI-MS as a versatile and efficient approach to study toxic heavy metals in biological systems.Key words: heavy metals, amino acids, electrospray ionization mass spectrometry.

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