Ultrasensitive Glucose Detection From Tears And Saliva Through Integrated Glucose Oxidase-Coupled DNAzyme and CRISPR-Cas12a

The Analyst ◽  
2021 ◽  
Author(s):  
Caiying Zhang ◽  
Hongfeng Yao ◽  
Qiang Ma ◽  
Bin Yu

The accurate and sensitive detection of glucose from secretory clinical samples, such as tears and saliva, remains a big challenge. In this research, a novel ultrasensitive glucose detection method composed...

The Analyst ◽  
2019 ◽  
Vol 144 (5) ◽  
pp. 1831-1839 ◽  
Author(s):  
Chengke Wang ◽  
Jiangyu Li ◽  
Rong Tan ◽  
Qingqing Wang ◽  
Zexiang Zhang

Preparation of peroxidase-mimic ZnFe2O4–carbon nanotube (CNT)–glucose oxidase (GOD) composite material for sensitive detection of glucose.


2021 ◽  
Author(s):  
Juan Tang ◽  
Liping Liu ◽  
Shan Gao ◽  
Jiao Qin ◽  
Xiaoxuan Liu ◽  
...  

A simple thermal aptasensing platform was devised for sensitive detection of organophosphate pesticides (malathion as a model target) based on the efficient self-heating reaction of a warm pad with a...


Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1893
Author(s):  
Sónia O. Pereira ◽  
Nuno F. Santos ◽  
Alexandre F. Carvalho ◽  
António J. S. Fernandes ◽  
Florinda M. Costa

Carbon-based electrodes have demonstrated great promise as electrochemical transducers in the development of biosensors. More recently, laser-induced graphene (LIG), a graphene derivative, appears as a great candidate due to its superior electron transfer characteristics, high surface area and simplicity in its synthesis. The continuous interest in the development of cost-effective, more stable and reliable biosensors for glucose detection make them the most studied and explored within the academic and industry community. In this work, the electrochemistry of glucose oxidase (GOx) adsorbed on LIG electrodes is studied in detail. In addition to the well-known electroactivity of free flavin adenine dinucleotide (FAD), the cofactor of GOx, at the expected half-wave potential of −0.490 V vs. Ag/AgCl (1 M KCl), a new well-defined redox pair at 0.155 V is observed and shown to be related to LIG/GOx interaction. A systematic study was undertaken in order to understand the origin of this activity, including scan rate and pH dependence, along with glucose detection tests. Two protons and two electrons are involved in this reaction, which is shown to be sensitive to the concentration of glucose, restraining its origin to the electron transfer from FAD in the active site of GOx to the electrode via direct or mediated by quinone derivatives acting as mediators.


Author(s):  
Georgina Fabregat ◽  
Sonia Lanzalaco ◽  
Jules Aït Saïd ◽  
Xavier Muñoz-Pascual ◽  
Jordi Llorca ◽  
...  

2021 ◽  
pp. 138050
Author(s):  
Hani Farhat ◽  
Joel Célier ◽  
Claude Forano ◽  
Christine Mousty

The Analyst ◽  
2021 ◽  
Author(s):  
Almas Shamaila Mohammed ◽  
Aniket Balapure ◽  
Mahammad Nanne Khaja ◽  
Ramakrishnan Ganesan ◽  
Jayati Ray Dutta

An Au NP based facile strategy for the rapid, early-stage, and sensitive detection of HCV RNA in clinical samples which avoids thiol tagging to the antisense oligonucleotide and expensive infrastructure is presented.


2016 ◽  
Vol 52 (29) ◽  
pp. 5187-5189 ◽  
Author(s):  
Ko-Keng Chang ◽  
Yi-Hong Cai ◽  
Chung-Hsuan Chen ◽  
Yi-Sheng Wang

A novel neutralization charge detection method enabling the fast and sensitive detection of ions under ambient and liquid phases.


Author(s):  
Jessica Le Gall ◽  
Catherine Dehainault ◽  
Camille Benoist ◽  
Alexandre Matet ◽  
Livia Lumbroso-Le Rouic ◽  
...  

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