Harnessing Long-lived Visible Phosphorescence to Eliminate Background Interference from Fingermark Images

The Analyst ◽  
2021 ◽  
Author(s):  
Ryan Andrew Parmenter ◽  
Kristen Clarke ◽  
William James Gee
Keyword(s):  
Background Fluorescence ◽  
Background Interference

Minimising background fluorescence can enhance the visible details of treated fingerprints. Here, a 4-tpt fingerprint powder exhibiting long-lived phosphorescence is applied to this end. The powder was found to suppress...

scholarly journals Fluorescence background discrimination by prebleaching.

1979 ◽  
Vol 27 (1) ◽  
pp. 96-101 ◽  
Author(s):  
T Hirschfeld
Keyword(s):  
Fluorescent Dyes ◽  
Fluorescence Decay ◽  
Free Form ◽  
Alternative Procedure ◽  
Background Fluorescence ◽  
Decay Lifetime ◽  
Fluorescent Molecules ◽  
Background Contrast

A number of electrooptical techniques are described that discriminate against background fluorescence in biologic staining, whether from sample background or unbound excess stain. These techniques are based on the fluorescent decay lifetime difference between bound stain and the sample background or between the bound stain its free form. The fluorescence decay lifetimes may be measured either directly or in a combination gated photometry scheme to substantially enhance the sample background contrast. An alternative procedure uses the photochemical bleaching of fluorescent dyes under intense exposure to time discriminate with higher selectivity, sensitivity and in a more convenient fashion between diverse fluorescent molecules.

Development of software and modification of Q-FISH protocol for estimation of individual telomere length in immunopathology

2017 ◽  
Vol 15 (02) ◽  
pp. 1650041 ◽  
Author(s):  
M. Sh. Barkovskaya ◽  
A. G. Bogomolov ◽  
N. Yu. Knauer ◽  
N. B. Rubtsov ◽  
V. A. Kozlov
Keyword(s):  
Telomere Length ◽  
Inflammatory Diseases ◽  
Quantitative Information ◽  
Computer Assisted ◽  
Metaphase Chromosomes ◽  
Important Indicator ◽  
Background Fluorescence ◽  
Immune Mediated ◽  
Light Effects ◽  
Assisted Analysis

Telomere length is an important indicator of proliferative cell history and potential. Decreasing telomere length in the cells of an immune system can indicate immune aging in immune-mediated and chronic inflammatory diseases. Quantitative fluorescent in situ hybridization (Q-FISH) of a labeled (C3TA[Formula: see text] peptide nucleic acid probe onto fixed metaphase cells followed by digital image microscopy allows the evaluation of telomere length in the arms of individual chromosomes. Computer-assisted analysis of microscopic images can provide quantitative information on the number of telomeric repeats in individual telomeres. We developed new software to estimate telomere length. The MeTeLen software contains new options that can be used to solve some Q-FISH and microscopy problems, including correction of irregular light effects and elimination of background fluorescence. The identification and description of chromosomes and chromosome regions are essential to the Q-FISH technique. To improve the quality of cytogenetic analysis after Q-FISH, we optimized the temperature and time of DNA-denaturation to get better DAPI-banding of metaphase chromosomes. MeTeLen was tested by comparing telomere length estimations for sister chromatids, background fluorescence estimations, and correction of nonuniform light effects. The application of the developed software for analysis of telomere length in patients with rheumatoid arthritis was demonstrated.

scholarly journals A sandwich-like strategy for the label-free detection of oligonucleotides by surface plasmon fluorescence spectroscopy (SPFS)

The Analyst ◽  
2016 ◽  
Vol 141 (20) ◽  
pp. 5784-5791 ◽  
Author(s):  
Qiang Su ◽  
Gilbert Nöll
Keyword(s):  
Fluorescence Spectroscopy ◽  
Surface Plasmon ◽  
Molecular Beacons ◽  
Label Free ◽  
Background Fluorescence ◽  
Detection Strategy ◽  
Cutting Surface ◽  
Label Free Detection ◽  
Lower Background

Cutting surface-bound optical molecular beacons results in a sandwich-like detection strategy with lower background fluorescence.

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