Rational design of fluorescent barcodes for suspension array through a simple simulation strategy

The Analyst ◽  
2021 ◽  
Author(s):  
Bo Zhang ◽  
Wan-Sheng Tang ◽  
Shou-Nian Ding
Keyword(s):  
Quantum Dots ◽  
Size Distribution ◽  
Fluorescence Intensity ◽  
Rational Design ◽  
Suspension Array ◽  
Simple Simulation ◽  
Simulation Strategy ◽  
High Fluorescence Intensity ◽  
High Fluorescence ◽  
Excellent Stability

The quantum dots (QDs)-encoded microbeads as optical barcode with high fluorescence intensity and fluorescence uniformity, excellent stability and dispersity are greatly important for suspension array (SA). However, the size distribution...

scholarly journals A flow cytometric assay of neutrophil degranulation.

1983 ◽  
Vol 31 (6) ◽  
pp. 737-744 ◽  
Author(s):  
W R Abrams ◽  
L W Diamond ◽  
A B Kane
Keyword(s):  
Flow Cytometry ◽  
Fluorescence Intensity ◽  
Ionophore A23187 ◽  
Flow Cytometric Assay ◽  
Elastase Activity ◽  
Flow Cytometric ◽  
Specific Granules ◽  
Neutrophil Degranulation ◽  
High Fluorescence Intensity ◽  
High Fluorescence

A quantitative assay of neutrophil degranulation was developed using flow cytometry. Dog neutrophils were purified to greater than 95% purity and viability by isopyknic density centrifugation in an isosmotic medium. These cells concentrated the fluorochrome acridine orange (AO) in their azurophilic granules, but not in specific granules. Also contained in the azurophilic granules are elastase, myeloperoxidase, and approximately 50% of the lysozyme activity. The fluorochrome was released concomitantly with elastase activity, as shown by flow cytometry, fluorescence microscopy, and biochemical assay in response to the ionophore A23187. By flow cytometry, unstimulated cells are distributed in a single broad peak of high fluorescence intensity. With increasing concentrations of A23187 (0.48-4.80 microM), a greater proportion of the cells shifted to a single peak of low fluorescence intensity. Few cells with intermediate fluorescence were observed. These analyses revealed that the neutrophils degranulated in a quantal, all-or-none response.

scholarly journals Copolymerization of Eu(TTA)3Phen doped styrene and methyl methacrylate nanoparticles and use in quantitative detection of pepsinogen

RSC Advances ◽  
2017 ◽  
Vol 7 (20) ◽  
pp. 12217-12223 ◽  
Author(s):  
Feng Wu ◽  
Mao Mao ◽  
Yu Cen ◽  
Hongtian Yang ◽  
Zhifeng Qin ◽  
...  
Keyword(s):  
Methyl Methacrylate ◽  
Fluorescence Intensity ◽  
Quantitative Detection ◽  
High Fluorescence Intensity ◽  
High Fluorescence

High fluorescence intensity nanoparticles were prepared by copolymerization of Eu(TTA)3Phen doped styrene and methyl methacrylate.

Benzothiazole integrated into a cryptand for ESIPT-based selective chemosensor for Zn2+ ions

2019 ◽  
Vol 48 (22) ◽  
pp. 7801-7808 ◽  
Author(s):  
Mayank Gupta ◽  
Sunanda Sahana ◽  
Vivekanand Sharma ◽  
Parimal K. Bharadwaj
Keyword(s):  
Excited State ◽  
Detection Limit ◽  
Proton Transfer ◽  
Fluorescence Intensity ◽  
Intramolecular Proton Transfer ◽  
High Fluorescence Intensity ◽  
High Fluorescence

A novel 2(2′-hydroxyphenyl) benzothiazole-based cryptand (L) exhibits high fluorescence intensity in the presence of Zn2+ ions by stopping the excited state intramolecular proton transfer (ESIPT) process with a detection limit of 0.20 μM.

scholarly journals D-Glucosamine Conjugation Accelerates the Labeling Efficiency of Quantum Dots in Osteoblastic Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Kazunari Igawa ◽  
Ming-Fang Xie ◽  
Hideki Ohba ◽  
Shizuka Yamada ◽  
Yoshihiko Hayashi
Keyword(s):  
Quantum Dots ◽  
Emission Spectra ◽  
Osteoblastic Cells ◽  
Core Size ◽  
Fluorescent Staining ◽  
Broad Absorption ◽  
Dramatic Decrease ◽  
High Fluorescence Intensity ◽  
High Fluorescence ◽  
Narrow Emission

Quantum dots (QDs) are useful imaging tools in the medical and biological fields due to their optical properties, such as a high fluorescence intensity, remarkable resistance to photobleaching, broad absorption spectra, and narrow emission spectra. This is the first study to investigate the uptake of carboxylated QDs conjugated with D-glucosamine (core size: approximately 3 nm, final modified size: 20–30 nm) into cultured osteoblastic cells. The QDs attached to the cell surface and were transported into the cytoplasm within approximately three hours of culture, whose process was clearly demonstrated using specific fluorescent staining of the cell membrane. Although the intranuclear distribution was not observed, a dramatic decrease in the transfer of quantum dots into the cytoplasm was recognized after approximately seven days of culture. Other interesting phenomena include the escape of the quantum dots from lysosomes in the cytoplasm, as confirmed by the merging of both QD fluorescence and specific fluorescent staining of lysosomes in the cytoplasm. These findings suggest that D-glucosamine conjugation enhances proton absorption in acid organelles and promotes the lysosomal escape of QDs.

Preparation of fluorescent polystyrene nanoparticles mediated by a multi-functional amphiphilic iridium complex under visible light irradiation in aqueous solution

2020 ◽  
Vol 11 (4) ◽  
pp. 795-799
Author(s):  
Zhen Lu ◽  
Sili Yi ◽  
Mingqin Shangguan ◽  
Xingzong Jiang ◽  
Pan Li ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Visible Light ◽  
Fluorescence Intensity ◽  
Visible Light Irradiation ◽  
Light Irradiation ◽  
Iridium Complex ◽  
Polystyrene Nanoparticles ◽  
High Fluorescence Intensity ◽  
High Fluorescence

Amphiphilc iridium(iii) complex has been synthesized and served as both a photoinitiator and a surfactant for the preparation of nanoparticles with high fluorescence intensity and uniform morphology by visible light irradiation of styrene in aqueous.

scholarly journals Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence

2014 ◽  
Vol 64 (1) ◽  
pp. 15-28 ◽  
Author(s):  
Bahia Abbas Moussa ◽  
Marianne Alphonse Mahrouse ◽  
Mahmoud Ali Hassan ◽  
Michael Gamal Fawzy
Keyword(s):  
Fluorescence Intensity ◽  
Pharmaceutical Formulations ◽  
Charge Transfer Complexes ◽  
Native Fluorescence ◽  
Factors Affecting ◽  
Fluorescence Techniques ◽  
Spectrofluorimetric Determination ◽  
High Fluorescence Intensity ◽  
High Fluorescence

Abstract Quinone-based fluorophores and enhanced native fluorescence techniques were applied for a fast quantitative analysis of gemifloxacin mesylate (GEM) and linezolid (LIN) in pharmaceutical formulations. For this purpose, three sensitive, accurate and precise spectrofluorimetric methods were developed. GEM, as an n-electron donor, reacts with 7,7,8,8-tetracyanoquinodimethane (method A) and 2,5-dichloro-3,6-dihydroxy-p-benzoquinone (method B) as п-electron acceptors, forming charge transfer complexes that exhibit high fluorescence intensity at 441 and 390 nm upon excitation at 260 and 339 nm, respectively. Method C depends on measurement of enhanced native fluorescence of LIN in phosphate buffer (pH 5) at 380 nm upon excitation at 260 nm. Experimental factors affecting fluorescence intensity were optimized. Linearity was obtained over concentration ranges 50-500, 10-60 and 20-400 ng mL-1 for methods A, B and C, respectively. The developed methods were validated and successfully applied for determination of the cited drugs in tablets.

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