Digitonin-facilitated delivery of imaging probes enables single-cell analysis of AKT signalling activities in suspension cells

The Analyst ◽  
2021 ◽  
Author(s):  
Siwen Wang ◽  
Nicole G. Perkins ◽  
Fei Ji ◽  
Rohit Chaudhuri ◽  
Zhili Guo ◽  
...  
Keyword(s):  
Single Cell ◽  
Cyclic Peptide ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Suspension Cells ◽  
Time Resolved ◽  
Imaging Probes ◽  
Single Cell Profiling

Digitonin allows the delivery of cyclic peptide-based imaging probes into suspension cells. This method enables time-resolved single-cell profiling of AKT signalling activities.

Single cell analysis for elucidating cellular uptake and transport of cobalt curcumin complex with detection by time-resolved ICPMS

2019 ◽  
Vol 1066 ◽  
pp. 13-20 ◽  
Author(s):  
Qi-Xuan Sun ◽  
Xing Wei ◽  
Shang-Qing Zhang ◽  
Ming-Li Chen ◽  
Ting Yang ◽  
...  
Keyword(s):  
Single Cell ◽  
Cellular Uptake ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Time Resolved ◽  
Uptake And Transport

scholarly journals Dynamic Real-Time Deformability Cytometry - Time-Resolved Mechanical Single Cell Analysis at 100 Cells/s

2020 ◽  
Vol 118 (3) ◽  
pp. 605a
Author(s):  
Bob Fregin ◽  
Fabian Czerwinski ◽  
Doreen Biedenweg ◽  
Salvatore Girardo ◽  
Stefan Groß ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Time Resolved ◽  
Deformability Cytometry

scholarly journals Time-resolved single-cell analysis of Brca1 associated mammary tumourigenesis reveals aberrant differentiation of luminal progenitors

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Karsten Bach ◽  
Sara Pensa ◽  
Marija Zarocsinceva ◽  
Katarzyna Kania ◽  
Julie Stockis ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Genetically Engineered ◽  
Cell Of Origin ◽  
Loss Of Function ◽  
Time Resolved ◽  
Genetically Engineered Mouse Models ◽  
Tumour Formation ◽  
Luminal Progenitor Cell ◽  
Single Cell Profiling

AbstractIt is unclear how genetic aberrations impact the state of nascent tumour cells and their microenvironment. BRCA1 driven triple negative breast cancer (TNBC) has been shown to arise from luminal progenitors yet little is known about how BRCA1 loss-of-function (LOF) and concomitant mutations affect the luminal progenitor cell state. Here we demonstrate how time-resolved single-cell profiling of genetically engineered mouse models before tumour formation can address this challenge. We found that perturbing Brca1/p53 in luminal progenitors induces aberrant alveolar differentiation pre-malignancy accompanied by pro-tumourigenic changes in the immune compartment. Unlike alveolar differentiation during gestation, this process is cell autonomous and characterised by the dysregulation of transcription factors driving alveologenesis. Based on our data we propose a model where Brca1/p53 LOF inadvertently promotes a differentiation program hardwired in luminal progenitors, highlighting the deterministic role of the cell-of-origin and offering a potential explanation for the tissue specificity of BRCA1 tumours.

Author response for "Immunocyte single cell analysis of vaccine‐induced narcolepsy"

2020 ◽  
Author(s):  
Alexander Lind ◽  
Falastin Salami ◽  
Anne‐Marie Landtblom ◽  
Lars Palm ◽  
Åke Lernmark ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Author Response ◽  
Cell Analysis
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