Label-free E. coli detection based on enzyme assay and microfluidic slipchip

The Analyst ◽  
2021 ◽  
Author(s):  
Gaozhe Cai ◽  
Wenshuai Wu ◽  
Shilun Feng ◽  
Yuanjie Liu
Keyword(s):  
Enzyme Assay ◽  
Label Free ◽  
E Coli ◽  
Specific Target ◽  
Label Free Detection ◽  
Target Analyte

An enzyme assay based method in microfluidic slipchip was proposed for rapid and label-free detection of E. coli. The specific target analyte of E. coli was β-D-Glucuronidase (GUS) which could...

Functionalized Polymers as Receptors for Detection of Cells

2011 ◽  
Vol 64 (9) ◽  
pp. 1256 ◽  
Author(s):  
Miroslava Polreichova ◽  
Usman Latif ◽  
Franz L. Dickert
Keyword(s):  
Soft Lithography ◽  
Quartz Crystal ◽  
Coli Strain ◽  
Yeast Cells ◽  
Label Free ◽  
Sensitive Material ◽  
E Coli ◽  
Label Free Detection ◽  
Quartz Crystal Microbalances ◽  
Cell Strains

Mass sensitive sensors were applied for fast and label-free detection of bio-analytes. Robust and miniaturized sensor devices were fabricated by combining bio-mimetic imprinted surfaces with quartz crystal microbalances for the analysis of yeast and bacteria cells. These sensors allow us to differentiate between different growing stages of yeast cells. Moreover, the viability of cells was detected by structuring quartz crystal microbalance electrodes like a grid. Artificial yeast cells were produced to pattern the recognition layer, giving reversible enrichment of the respective bio-analytes. This approach was followed to ensure the reproducibility of the identical sensitive material in each case, because the properties of each cell depend on its growth stage, which varies over time. The strategy was further applied to develop a sensitive system for Escherichia coli. Structuring of these materials by soft lithography allows differentiation between cell strains, e.g. E. coli (strain W & B) with a five-fold selectivity.

scholarly journals Label-Free Detection of E. coli O157:H7 DNA Using Light-Addressable Potentiometric Sensors with Highly Oriented ZnO Nanorod Arrays

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5473
Author(s):  
Yulan Tian ◽  
Tao Liang ◽  
Ping Zhu ◽  
Yating Chen ◽  
Wei Chen ◽  
...  
Keyword(s):  
Quality Control ◽  
Potentiometric Sensors ◽  
Zno Nanorod ◽  
Nanorod Arrays ◽  
Zno Nanorod Arrays ◽  
Label Free ◽  
Functional Surface ◽  
Bacterial Dna ◽  
E Coli ◽  
Label Free Detection

The detection of bacterial deoxyribonucleic acid (DNA) is of great significance in the quality control of food and water. In this study, a light-addressable potentiometric sensor (LAPS) deposited with highly oriented ZnO nanorod arrays (NRAs) was used for the label-free detection of single-stranded bacterial DNA (ssDNA). A functional, sensitive surface for the detection of Escherichia coli (E. coli) O157:H7 DNA was prepared by the covalent immobilization of the specific probe single-stranded DNA (ssDNA) on the LAPS surface. The functional surface was exposed to solutions containing the target E. coli ssDNA molecules, which allowed for the hybridization of the target ssDNA with the probe ssDNA. The surface charge changes induced by the hybridization of the probe ssDNA with the target E. coli ssDNA were monitored using LAPS measurements in a label-free manner. The results indicate that distinct signal changes can be registered and recorded to detect the target E. coli ssDNA. The lower detection limit of the target ssDNA corresponded to 1.0 × 102 colony forming units (CFUs)/mL of E. coli O157:H7 cells. All the results demonstrate that this DNA biosensor, based on the electrostatic detection of ssDNA, provides a novel approach for the sensitive and effective detection of bacterial DNA, which has promising prospects and potential applications in the quality control of food and water.

Evanescent wave absorbance based fiber optic biosensor for label-free detection of E. coli at 280nm wavelength

2011 ◽  
Vol 26 (7) ◽  
pp. 3367-3370 ◽  
Author(s):  
Reshma Bharadwaj ◽  
V.V.R. Sai ◽  
Kamini Thakare ◽  
Arvind Dhawangale ◽  
Tapanendu Kundu ◽  
...  
Keyword(s):  
Evanescent Wave ◽  
Fiber Optic ◽  
Label Free ◽  
E Coli ◽  
Label Free Detection

scholarly journals Detection of Escherichia coli bacteria by impact electrochemistry

The Analyst ◽  
2018 ◽  
Vol 143 (20) ◽  
pp. 4840-4843 ◽  
Author(s):  
Rosa A. S. Couto ◽  
Lifu Chen ◽  
Sabine Kuss ◽  
Richard G. Compton
Keyword(s):  
Escherichia Coli ◽  
Label Free ◽  
Redox Species ◽  
E Coli ◽  
Label Free Detection ◽  
Escherichia Coli Bacteria ◽  
First Time ◽  
Phenylene Diamine

Employing the redox species N,N,N′,N′-tetramethyl-para-phenylene-diamine (TMPD), the label-free detection of E. coli, based on an electrochemical “on”-signal during impact electrochemistry, is reported for the first time.

Glycosylation of Quinone-Fused Polythiophene for Reagentless and Label-Free Detection of E. coli

2015 ◽  
Vol 87 (3) ◽  
pp. 1560-1568 ◽  
Author(s):  
Fen Ma ◽  
Abdul Rehman ◽  
Haiying Liu ◽  
Jingtuo Zhang ◽  
Shilei Zhu ◽  
...  
Keyword(s):  
Label Free ◽  
E Coli ◽  
Label Free Detection

scholarly journals Rapid label-free detection of E. coli using antimicrobial peptide assisted impedance spectroscopy

2015 ◽  
Vol 7 (23) ◽  
pp. 9744-9748 ◽  
Author(s):  
Keren Jiang ◽  
Hashem Etayash ◽  
Sarfuddin Azmi ◽  
Selvaraj Naicker ◽  
Mahtab Hassanpourfard ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Impedance Spectroscopy ◽  
Antimicrobial Peptide ◽  
Rapid Detection ◽  
Sensor Array ◽  
Label Free ◽  
E Coli ◽  
Sensor Platform ◽  
Label Free Detection

A compact, label-free sensor array for rapid detection ofEscherichia coli(E. coli) using antimicrobial peptide assisted impedimetric sensor platform.

Cost-effective flow-through nanohole array-based biosensing platform for the label-free detection of uropathogenic E. coli in real time

2018 ◽  
Vol 106 ◽  
pp. 105-110 ◽  
Author(s):  
Juan Gomez-Cruz ◽  
Srijit Nair ◽  
Angel Manjarrez-Hernandez ◽  
Sandra Gavilanes-Parra ◽  
Gabriel Ascanio ◽  
...  
Keyword(s):  
Real Time ◽  
Cost Effective ◽  
Label Free ◽  
Nanohole Array ◽  
E Coli ◽  
Label Free Detection ◽  
Flow Through
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