A general purpose MALDI matrix for the analyses of small organic, peptide and protein molecules

The Analyst ◽  
2021 ◽  
Author(s):  
Hao Gu ◽  
Kang Ma ◽  
Weiqian Zhao ◽  
Lirong Qiu ◽  
Wei Xu
Keyword(s):  
Inorganic Material ◽  
Laser Desorption ◽  
General Purpose ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Protein Molecules ◽  
Organic Matrices ◽  
P Matrix ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has been widely applied for the analysis of large biomolecules. The emergence of inorganic material substrates and new organic matrices extend the use...

scholarly journals 1,8-Di(piperidinyl)-naphthalene – rationally designed MAILD/MALDI matrix for metabolomics and imaging mass spectrometry

RSC Advances ◽  
2016 ◽  
Vol 6 (79) ◽  
pp. 75073-75081 ◽  
Author(s):  
Jerrit Weißflog ◽  
Aleš Svatoš
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Imaging Mass Spectrometry ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
P Matrix ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of small molecules requires special matrices, which do not generate interfering signals below m/z 500.

MALDI mass spectrometric imaging meets “omics”: recent advances in the fruitful marriage

The Analyst ◽  
2015 ◽  
Vol 140 (17) ◽  
pp. 5806-5820 ◽  
Author(s):  
A. C. Crecelius ◽  
U. S. Schubert ◽  
F. von Eggeling
Keyword(s):  
Laser Desorption ◽  
Mass Spectrometric ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Mass Spectrometric Imaging ◽  
Desorption Ionization ◽  
Recent Advances ◽  
P Matrix ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI MSI) is a method that allows the investigation of the molecular content of surfaces, in particular, tissues, within its morphological context.

scholarly journals Synthesis and Matrix Properties of α-Cyano-5-phenyl-2,4-pentadienic Acid (CPPA) for Intact Proteins Analysis by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 6054
Author(s):  
Antonio Monopoli ◽  
Angelo Nacci ◽  
Tommaso R. I. Cataldi ◽  
Cosima D. Calvano
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Protein Analysis ◽  
Ionization Mass Spectrometry ◽  
Intact Protein ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

The effectiveness of a synthesized matrix, α-cyano-5-phenyl-2,4-pentadienic acid (CPPA), for protein analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in complex samples such as foodstuff and bacterial extracts, is demonstrated. Ultraviolet (UV) absorption along with laser desorption/ionization mass spectrometry (LDI-MS) experiments were systematically conducted in positive ion mode under standard Nd:YLF laser excitation with the aim of characterizing the matrix in terms of wavelength absorption and proton affinity. Besides, the results for standard proteins revealed that CPPA significantly enhanced the protein signals, reduced the spot-to-spot variability and increased the spot homogeneity. The CPPA matrix was successful employed to investigate intact microorganisms, milk and seed extracts for protein profiling. Compared to conventional matrices such as sinapinic acid (SA), α-cyano-4-hydroxycinnamic acid (CHCA) and 4-chloro-α-cyanocinnamic acid (CClCA), CPPA exhibited better signal-to-noise (S/N) ratios and a uniform response for most examined proteins occurring in milk, hazelnut and in intact bacterial cells of E. coli. These findings not only provide a reactive proton transfer MALDI matrix with excellent reproducibility and sensitivity, but also contribute to extending the battery of useful matrices for intact protein analysis.

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