A polyA DNA probe-based ultra-sensitive and structure-distinguishable electrochemical biosensor for the analysis of RNAi transgenic maize

The Analyst ◽  
2021 ◽  
Author(s):  
Li Xu ◽  
Jiawei Qi ◽  
Yanli Wen ◽  
Wen Liang ◽  
Lele Wang ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Secondary Structure ◽  
Electrochemical Biosensor ◽  
Dna Probe ◽  
Transgenic Maize ◽  
Total Rna ◽  
Probe System ◽  
Reporter Probe ◽  
Excellent Selectivity

A multiple reporter probe system (MRP) was applied to open the secondary structure and hybridize with a long RNA. Capability of quantification and structural analysis of long RNA was demonstrated. Excellent selectivity was achieved when analyzing extracted total RNA samples.

Ratiometric Electrochemical Biosensor for the Sensitive Determination of DNA by a Hairpin DNA Probe

2021 ◽  
pp. 1-11
Author(s):  
Haixia Wang ◽  
Xuejiao Wang ◽  
Mingyuan Zhao ◽  
Chao Shi ◽  
Cuiping Ma
Keyword(s):  
Electrochemical Biosensor ◽  
Dna Probe ◽  
Hairpin Dna ◽  
Sensitive Determination

Analogous modified DNA probe and immune competition method-based electrochemical biosensor for RNA modification

2018 ◽  
Vol 114 ◽  
pp. 72-77 ◽  
Author(s):  
Tao Dai ◽  
Qinli Pu ◽  
Yongcan Guo ◽  
Chen Zuo ◽  
Shulian Bai ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Dna Probe ◽  
Rna Modification ◽  
Modified Dna

Eliminating the secondary structure of targeting strands for enhancement of DNA probe based low-abundance point mutation detection

2019 ◽  
Vol 1075 ◽  
pp. 137-143
Author(s):  
Zhihao Ming ◽  
Qianzhi Chen ◽  
Na Chen ◽  
Meng Lin ◽  
Na Liu ◽  
...  
Keyword(s):  
Secondary Structure ◽  
Point Mutation ◽  
Mutation Detection ◽  
Dna Probe

An electrochemical sensing platform based on local repression of electrolyte diffusion for single-step, reagentless, sensitive detection of a sequence-specific DNA-binding protein

The Analyst ◽  
2014 ◽  
Vol 139 (9) ◽  
pp. 2193-2198 ◽  
Author(s):  
Yun Zhang ◽  
Fang Liu ◽  
Jinfang Nie ◽  
Fuyang Jiang ◽  
Caibin Zhou ◽  
...  
Keyword(s):  
Dna Binding ◽  
Electrode Surface ◽  
Electrochemical Biosensor ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Single Step ◽  
Dna Probe ◽  
Electrochemical Sensing ◽  
Sensing Platform ◽  
First Time

This paper describes for the first time an electrochemical biosensor, which employs a DNA probe modified with a redox tag close to electrode surface, for picomolar detection of a sequence-specific DNA-binding protein.

Preparation of enzyme-conjugated DNA probe and application to the universal probe system

1991 ◽  
Vol 198 (1) ◽  
pp. 75-79 ◽  
Author(s):  
Satoru Nakagami ◽  
Hironari Matsunaga ◽  
Norihiro Oka ◽  
Akio Yamane
Keyword(s):  
Dna Probe ◽  
Probe System

scholarly journals Structural and compositional changes of whey protein and blueberry juice fermented using Lactobacillus plantarum or Lactobacillus casei during fermentation

RSC Advances ◽  
2021 ◽  
Vol 11 (42) ◽  
pp. 26291-26302
Author(s):  
Wang Wen-qiong ◽  
Zhang Jie-long ◽  
Yu Qian ◽  
Zhou Ji-yang ◽  
Lu Mao-lin ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Secondary Structure ◽  
Whey Protein ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Casei ◽  
Schematic Diagram ◽  
Blueberry Juice ◽  
Structure Changes ◽  
Compositional Changes

A possible schematic diagram to show how the secondary structure changes which the whey protein and blueberry juice interaction after Lactobacillus casei or Lactobacillus plantarum fermentation was inferred from the structural analysis results.

scholarly journals Optimization of probe immobilization by covalent links on gold nanowire based- electrochemical biosensor

2015 ◽  
Vol 18 (3) ◽  
pp. 135-144
Author(s):  
Tien Huu Cao ◽  
Linh Van Ha ◽  
Hieu Van Le
Keyword(s):  
Electrode Surface ◽  
Surface Density ◽  
Gold Electrode ◽  
Electrochemical Biosensor ◽  
Dna Probe ◽  
Molar Ratio ◽  
Dna Biosensors ◽  
Gold Nanowire ◽  
Molecular Density ◽  
Probe Concentration

The optimization of DNA probe immobilisation on gold electrode surface is very important to develop DNA biosensors. In this study, we conducted an experiment to determine the optimal concentration of probe attached on the electrodes and probe immobilization agent (mercaptohexanol) for maximum hybridization efficacy. We have used a method to control the surface density of DNA probe by annealing probe modified by thiol and mercaptohexanol. With linear relationship between molar ratio and surface density of probe, by controlling probe concentration in sensor fabricating process, we can determine the molecular density of DNA probes on electrode surface. The results show that probe concentration 500 nM and 1.5 mM mercaptohaxenol are optimal for hybidization with DNA target.

A multipedal DNA walker for amplified detection of tumor exosomes

2020 ◽  
Vol 56 (37) ◽  
pp. 4982-4985 ◽  
Author(s):  
Peng Miao ◽  
Yuguo Tang
Keyword(s):  
Electrochemical Biosensor ◽  
Limit Of Detection ◽  
Dna Walker ◽  
Tumor Exosomes ◽  
Excellent Selectivity

A novel electrochemical biosensor for exosomes is proposed based on a multipedal DNA walking strategy. A limit of detection down to 6/μL is obtained with excellent selectivity.

scholarly journals Current perspectives on RNA secondary structure probing

2014 ◽  
Vol 42 (4) ◽  
pp. 1251-1255 ◽  
Author(s):  
Julia Kenyon ◽  
Liam Prestwood ◽  
Andrew Lever
Keyword(s):  
Structural Analysis ◽  
Secondary Structure ◽  
Rna Structure ◽  
Rna Secondary Structure ◽  
Biological Systems ◽  
Present Review ◽  
Structure Probing ◽  
Recent Advances ◽  
Near Future

The range of roles played by structured RNAs in biological systems is vast. At the same time as we are learning more about the importance of RNA structure, recent advances in reagents, methods and technology mean that RNA secondary structural probing has become faster and more accurate. As a result, the capabilities of laboratories that already perform this type of structural analysis have increased greatly, and it has also become more widely accessible. The present review summarizes established and recently developed techniques. The information we can derive from secondary structural analysis is assessed, together with the areas in which we are likely to see exciting developments in the near future.

An Enzyme-Based DNA Probe System

1987 ◽  
Vol 501 (1 Enzyme Engine) ◽  
pp. 276-282
Author(s):  
EDMUND ZIOMEK ◽  
PIOTR STEPIEN ◽  
ROSS E. WILLIAMS
Keyword(s):  
Dna Probe ◽  
Probe System
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