Size-dependent optical extinction of MoS2 nanosheets and their aptamer-induced dispersion behavior for the label-free detection of Escherichia coli O157:H7

The Analyst ◽  
2021 ◽  
Author(s):  
Jiye Li ◽  
Jie Wang
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Label Free ◽  
Mos2 Nanosheets ◽  
Optical Extinction ◽  
Size Dependent ◽  
Dispersion Behavior ◽  
Label Free Detection ◽  
First Time

For the first time, we have revealed the size-dependent extinction response of MoS2-NS aggregation, the phenomenon of which has been employed as a sensing strategy for the label-free detection of Escherichia coli O157:H7.

scholarly journals Novel impedimetric aptasensor for label-free detection of Escherichia coli O157:H7

2018 ◽  
Vol 255 ◽  
pp. 2988-2995 ◽  
Author(s):  
Sergi Brosel-Oliu ◽  
Rubén Ferreira ◽  
Naroa Uria ◽  
Natalia Abramova ◽  
Raimundo Gargallo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Label Free ◽  
Label Free Detection

scholarly journals Detection of Escherichia coli bacteria by impact electrochemistry

The Analyst ◽  
2018 ◽  
Vol 143 (20) ◽  
pp. 4840-4843 ◽  
Author(s):  
Rosa A. S. Couto ◽  
Lifu Chen ◽  
Sabine Kuss ◽  
Richard G. Compton
Keyword(s):  
Escherichia Coli ◽  
Label Free ◽  
Redox Species ◽  
E Coli ◽  
Label Free Detection ◽  
Escherichia Coli Bacteria ◽  
First Time ◽  
Phenylene Diamine

Employing the redox species N,N,N′,N′-tetramethyl-para-phenylene-diamine (TMPD), the label-free detection of E. coli, based on an electrochemical “on”-signal during impact electrochemistry, is reported for the first time.

Rapid, sensitive and label-free detection of Shiga-toxin producing Escherichia coli O157 using carbon nanotube biosensors

2012 ◽  
Vol 32 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Sowmya Subramanian ◽  
Konrad H. Aschenbach ◽  
Jennifer P. Evangelista ◽  
Mohamed Badaoui Najjar ◽  
Wenxia Song ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carbon Nanotube ◽  
Shiga Toxin ◽  
Escherichia Coli O157 ◽  
Label Free ◽  
Label Free Detection

Label-Free Detection of Escherichia coli from Mixed Bacterial Cultures Using Bacteriophage T4 on Plasmonic Fiber-Optic Sensor

ACS Sensors ◽  
2021 ◽  
Author(s):  
Pallavi Halkare ◽  
Nirmal Punjabi ◽  
Jigme Wangchuk ◽  
Santhosh Madugula ◽  
Kiran Kondabagil ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fiber Optic ◽  
Bacteriophage T4 ◽  
Fiber Optic Sensor ◽  
Label Free ◽  
Bacterial Cultures ◽  
Optic Sensor ◽  
Label Free Detection

A capacitive DNA sensor for sensitive detection of Escherichia coli O157:H7 in potable water based on the z3276 genetic marker: fabrication and analytical performance

The Analyst ◽  
2020 ◽  
Vol 145 (6) ◽  
pp. 2267-2278 ◽  
Author(s):  
Rehan Deshmukh ◽  
Arun Kumar Prusty ◽  
Utpal Roy ◽  
Sunil Bhand
Keyword(s):  
Escherichia Coli ◽  
Genetic Marker ◽  
Potable Water ◽  
Analytical Performance ◽  
Sensitive Detection ◽  
Escherichia Coli O157 ◽  
Dna Sensor ◽  
Label Free ◽  
Dna Sensing ◽  
Water Based

We report a label-free biosensor for the detection of Escherichia coli O157:H7 ATCC 43895 in potable water using a newly designed DNA sensing probe targeting the z3276 genetic marker.

10-Minute Assay for Detecting Escherichia coli O157:H7 in Ground Beef Samples Using Piezoelectric-Excited Millimeter-Size Cantilever Sensors

2007 ◽  
Vol 70 (7) ◽  
pp. 1670-1677 ◽  
Author(s):  
DAVID MARALDO ◽  
RAJ MUTHARASAN
Keyword(s):  
Escherichia Coli ◽  
Sample Preparation ◽  
Ground Beef ◽  
Low Ph ◽  
Frequency Change ◽  
Escherichia Coli O157 ◽  
Label Free ◽  
Cantilever Sensors ◽  
Ph Buffer ◽  
Phosphate Buffered Saline

We detected Escherichia coli O157:H7 (EC) at approximately 10 cells per ml in spiked ground beef samples in 10 min using piezoelectric-excited millimeter-size cantilever (PEMC) sensors. The composite PEMC sensors have a sensing area of 2mm2 and are prepared by immobilizing a polyclonal antibody specific to EC on the sensing surface. Ground beef (2.5 g) was spiked with EC at 10 to 10,000 cells per ml in phosphate-buffered saline (PBS). One milliliter of supernatant was removed from the blended samples and used to perform the detection experiments. The total resonant frequency change obtained for the inoculated samples was 138 ± 9, 735 ± 23, 2,603 ± 51, and 7,184 ± 606 Hz, corresponding to EC concentrations of 10, 100, 1,000, and 10,000 cells per ml, respectively. EC was detected in the sample solution within the first 10 min. The responses of the sensor to positive, negative, and buffer controls were 36 ± 6, 27 ± 2, and 2 ± 7 Hz, respectively. Verification of EC attachment was confirmed by low-pH buffer release (PBS-HCl, pH 2.2), microscopy, and second antibody EC binding postdetection. The results indicate that PEMC sensors can reliably detect EC at less than 10 cells per ml in 10 min without sample preparation and with label-free reagents.

scholarly journals Terahertz Detection of Zika Viruses

2020 ◽  
Author(s):  
Subhashish Dolai ◽  
Massood Tabib-Azar
Keyword(s):  
Electromagnetic Waves ◽  
Direct Detection ◽  
Petri Dish ◽  
Label Free ◽  
Low Loss ◽  
Terahertz Detection ◽  
Label Free Detection ◽  
Terahertz Transmission ◽  
First Time ◽  
Thick Glass

Our main objective in this work was to examine the possibility of non-intrusive, label-free, detection of whole Zika viruses using terahertz signals with or without a targeting/binding oligonucleotide (aptamers). We report for the first time the use of terahertz electromagnetic waves (0.75 THz – 1.1 THz) to detect Zika viruses. The Zika/aptamer complexes showed a reproducible terahertz reflection coefficient minimum at 1.064 THz while the Zika virus’s reflection minimum was at 1.073 THz. Of different substrates we examined, the polyester petri dish provided a very low loss and excellent terahertz transmission. To increase the interaction between the terahertz signal and the sample we also used polyester microbeads coated with aptamers. We then measured the terahertz reflection from the microbeads as a function of Zika concentration. The resulting terahertz Zika sensor had sensitivity of 63 Hz/Zika and minimum detectable signal of ~ 16x103 Zika. Other substrates such as Graphene on polyethylene terephthalate (PET), 50 nm-thick gold film on polycarbonate, thin (30 um-thick) glass slide and Teflon were also examined. Graphene substrate enabled direct detection of the Zika without any aptamers.

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