Cell-free scaffold functionalized with bionic cartilage acellular matrix microspheres to enhance microfracture treatment of articular cartilage defects

2021 ◽  
Author(s):  
Jun Liu ◽  
Yan Lu ◽  
Fei Xing ◽  
Jie Liang ◽  
Qiguang Wang ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Subchondral Bone ◽  
Cartilage Lesion ◽  
Cartilage Defects ◽  
Acellular Matrix ◽  
Articular Cartilage Lesion ◽  
Articular Cartilage Defects

Microfracture surgery remains the most popular treatment for articular cartilage lesion in clinical, but often lead to the formation of inferior fibrocartilage tissue and damage to subchondral bone. To overcome...

scholarly journals Effect of Vertical or Beveled Chondral Defect Creation on Rim Deformation and Contact

Cartilage ◽  
2018 ◽  
Vol 10 (2) ◽  
pp. 222-228 ◽  
Author(s):  
Adam B. Yanke ◽  
Megan L. Konopka ◽  
Davietta C. Butty ◽  
Maximilian A. Meyer ◽  
Eric J. Cotter ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Subchondral Bone ◽  
Rank Test ◽  
Cartilage Defects ◽  
Micro Computed Tomography ◽  
Bone Contact ◽  
Defect Center ◽  
Tibial Cartilage ◽  
Contact Width ◽  
Articular Cartilage Defects

Objective To determine biomechanical effects of knee cartilage defect perimeter morphology based on cartilage strain and opposing subchondral bone contact. Design Articular cartilage defects were created in 5 bovine femoral condyles: group 1, 45° inner bevel with 8-mm rim; group 2, vertical with 8-mm rim; and group 3, 45° outer bevel with 8-mm base. Samples were placed into a custom-machined micro–computed tomography tube and subjected to 800 N of axial loading. DICOM data were used to calculate cartilage thickness 4 and 6 mm from the center, distance between tibial cartilage surface and femoral subchondral bone, and contact width between tibial cartilage and subchondral bone. Strain 4 mm from the center and both absolute and change in distance (mm) to subchondral bone were compared between groups 1 and 2 using paired t tests. Strain at 6 mm and distance changed, loaded distance, and contact width (mm) were compared between groups using the Friedman test with post hoc analysis using Wilcoxon signed rank test. Results No significant differences in rim strain were noted between groups 1 and 2 at 4 mm ( P = 0.10) and between groups 1, 2, and 3 at 6 mm ( P = 0.247) from the defect center. The loaded distance was significantly different between groups 1 and 3 ( P = 0.013). No significant change in distance to the subchondral bone was found between groups ( P = 0.156). The difference in subchondral bone contact area approached but did not reach significance ( P = 0.074). Conclusion When debriding focal articular cartilage defects, establishment of an inner bevel decreases tissue deformation and contact with opposing subchondral bone.

scholarly journals Rat perichondrium transplanted to articular cartilage defects forms articular-like, hyaline cartilage

2020 ◽  
Author(s):  
Zelong Dou ◽  
Daniel Muder ◽  
Marta Baroncelli ◽  
Ameya Bendre ◽  
Alexandra Gkourogianni ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Articular Cartilage ◽  
Subchondral Bone ◽  
Fluorescent Protein ◽  
Bone Marrow Cells ◽  
Hyaline Cartilage ◽  
Cartilage Defects ◽  
Trochlear Groove ◽  
Post Surgery ◽  
Articular Cartilage Defects

AbstractReconstruction of articular surfaces destroyed by infection or trauma is hampered by the lack of suitable graft tissues. Perichondrium autotransplants have been used for this purpose. However, the role of the transplanted perichondrium in the healing of resurfaced joints have not been investigated. Perichondrial and periosteal tissues were harvested from rats hemizygous for a ubiquitously expressed enhanced green fluorescent protein (EGFP) transgene and transplanted into full-thickness articular cartilage defects at the trochlear groove of distal femur in wild-type littermates. As an additional control, cartilage defects were left without a transplant (no transplant control). Distal femurs were collected 3, 14, 56, 112 days after surgery. Transplanted cells and their progenies were readily detected in the defects of perichondrium and periosteum transplanted animals but not in defects left without a transplant. Perichondrium transplants expressed SOX9 and with time differentiated into a hyaline cartilage that expanded and filled out the defects with Col2a1-positive chondrocytes and a matrix rich in proteoglycans. Interestingly, at later timepoints the cartilaginous perichondrium transplants were actively remodeled into bone at the transplant-bone interface and at post-surgery day 112 EGFP-positive perichondrium cells at the articular surface were positive for Prg4. In addition, both perichondrium and periosteum transplants contributed cells to the subchondral bone and bone marrow, suggesting differentiation into osteoblast/osteocytes as well as bone marrow cells. In summary, we found that perichondrium transplanted to articular cartilage defects develops into an articular-like, hyaline cartilage that integrates with the subchondral bone, and is maintained for an extended time. The findings indicate that perichondrium is a suitable tissue for repair and engineering of articular cartilage.

scholarly journals A composite scaffold of Wharton’s jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee

2021 ◽  
Vol 32 (4) ◽  
Author(s):  
Zhong Li ◽  
Yikang Bi ◽  
Qi Wu ◽  
Chao Chen ◽  
Lu Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Articular Cartilage ◽  
Composite Scaffold ◽  
Biomechanical Properties ◽  
Cartilage Defects ◽  
Human Umbilical Cord ◽  
Composite Scaffolds ◽  
Articular Cartilage Defects

AbstractTo evaluate the performance of a composite scaffold of Wharton’s jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out. The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold. In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee. Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs. We found that in vitro, the Young’s modulus of the composite scaffold (WJ-CS) was higher than that of the WJ scaffold. In vivo, the composite scaffold loaded with hUCMSCs repaired rat cartilage defects better than did the WJ scaffold loaded with hUCMSCs. Both the scaffold and hUCMSCs showed low immunogenicity. These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs.

Comparative Study of the Reconstruction of Articular Cartilage Defects with Free Costal Perichondrial Grafts and Free Tibial Periosteal Grafts: An Experimental Study on Rabbits

1999 ◽  
Vol 65 (5) ◽  
pp. 402-407 ◽  
Author(s):  
A. Carranza-Bencano ◽  
M. Perez-Tinao ◽  
P. Ballesteros-Vazquez ◽  
J. R. Armas-Padron ◽  
A. Hevia-Alonso ◽  
...  
Keyword(s):  
Experimental Study ◽  
Articular Cartilage ◽  
Comparative Study ◽  
Cartilage Defects ◽  
Articular Cartilage Defects
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