Nanohydroxyapatite/polyamide 66 crosslinked with QK and BMP-2-derived peptide prevented femur nonunion in rats

2021 ◽  
Author(s):  
Ang Li ◽  
Jian Li ◽  
Zhengye Zhang ◽  
Zecheng Li ◽  
Hui Chi ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Polyamide 66 ◽  
Controlled Released

A dual-peptide controlled released system based on nHA/PA66 scaffold for enhancing bone regeneration.

scholarly journals A Dual Peptide Sustained-Release System Based on Nanohydroxyapatite/Polyamide 66 Scaffold for Synergistic-Enhancing Diabetic Rats’ Fracture Healing in Osteogenesis and Angiogenesis

2021 ◽  
Vol 9 ◽  
Author(s):  
Jian Li ◽  
Jiaxing Wei ◽  
Ang Li ◽  
Hongyu Liu ◽  
Jingxue Sun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Sustained Release ◽  
Fracture Healing ◽  
Bone Regeneration ◽  
Tube Formation ◽  
Diabetic Rats ◽  
Polyamide 66 ◽  
Release System ◽  
Covalent Grafting

Diabetes mellitus impairs fracture healing and function of stem cells related to bone regeneration; thus, effective bone tissue engineering therapies can intervene with those dysfunctions. Nanohydroxyapatite/polyamide 66 (n-HA/PA66) scaffold has been used in fracture healing, whereas the low bioactivity limits its further application. Herein, we developed a novel bone morphogenetic protein-2- (BMP-2) and vascular endothelial growth factor- (VEGF) derived peptides-decorated n-HA/PA66 (BVHP66) scaffold for diabetic fracture. The n-HA/PA66 scaffold was functionalized by covalent grafting of BMP-2 and VEGF peptides to construct a dual peptide sustained-release system. The structural characteristics and peptide release profiles of BVHP66 scaffold were tested by scanning electron microscopy, Fourier transform infrared spectroscopy, and fluorescence microscope. Under high glucose (HG) condition, the effect of BVHP66 scaffold on rat bone marrow mesenchymal stem cells’ (rBMSCs) adherent, proliferative, and differentiate capacities and human umbilical vein endothelial cells’ (HUVECs) proliferative and tube formation capacities was assessed. Finally, the BVHP66 scaffold was applied to fracture of diabetic rats, and its effect on osteogenesis and angiogenesis was evaluated. In vitro, the peptide loaded on the BVHP66 scaffold was in a sustained-release mode of 14 days. The BVHP66 scaffold significantly promoted rBMSCs’ and HUVECs’ proliferation and improved osteogenic differentiation of rBMSCs and tube formation of HUVECs in HG environment. In vivo, the BVHP66 scaffold enhanced osteogenesis and angiogenesis, rescuing the poor fracture healing in diabetic rats. Comparing with single peptide modification, the dual peptide-modified scaffold had a synergetic effect on bone regeneration in vivo. Overall, this study reported a novel BVHP66 scaffold with excellent biocompatibility and bioactive property and its application in diabetic fracture.

Sequential controlled-released dual-drug loaded scaffold for guided bone regeneration in a rat fenestration defect model

2017 ◽  
Vol 5 (37) ◽  
pp. 7701-7710 ◽  
Author(s):  
Zhenzhao Guo ◽  
Dongying Bo ◽  
Ping He ◽  
Hong Li ◽  
Gang Wu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Defect Model ◽  
Inflammatory Drug ◽  
Anti Inflammatory ◽  
Osseous Regeneration ◽  
Controlled Released ◽  
Dual Drug

A 3D multifunctional scaffold, which combines the merits of osseous regeneration and local anti-inflammatory drug delivery, has been developed for GBR.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

Nouveaux catalyseurs d'hydrogénation à base de platine déposé sur polyamide 66

1975 ◽  
Vol 72 ◽  
pp. 735-740 ◽  
Author(s):  
Jean René Bernard ◽  
Can Hoang-Van ◽  
Stanislas Jean Teichner
Keyword(s):  
Polyamide 66

Nouveaux catalyseurs d’hydrogénation à base platine déposé sur polyamide-66

1976 ◽  
Vol 73 ◽  
pp. 799-802 ◽  
Author(s):  
Jean-René Bernard ◽  
Can Hoang-Van ◽  
Stanislas Jean Teichner
Keyword(s):  
Polyamide 66

Placenta mesenchymal stem cells on a chorion scaffold as a potential osteogenic graft for peripartal bone regeneration

2008 ◽  
Vol 68 (S 01) ◽  
Author(s):  
S Mohr ◽  
BC Portmann-Lanz ◽  
A Schoeberlein ◽  
R Sager ◽  
DV Surbek
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration

Combination of novel two-photon photopolymerised scaffolds and bioactive elastin-like-recombinamers induce bone regeneration

2016 ◽  
Author(s):  
Carina Kampleitner ◽  
Gerhard Hildebrand ◽  
Klaus Liefeith ◽  
Constancio Gonzalez ◽  
Jose Carlos Rodriguez-Cabello ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Two Photon

SauFRa technique for the fixation of resorbable membranes in horizontal guided bone regeneration - a technical report

2020 ◽  
Author(s):  
Saurabh Mohan Kamat ◽  
Rakshit Khandeparker ◽  
Francis Akkara ◽  
Vikas Dhupar ◽  
Ashwin Mysore
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Novel Technique ◽  
Technical Report ◽  
Suturing Techniques ◽  
Graft Migration

Membrane fixation in guided bone regeneration (GBR) has been traditionally achieved using resorbable pins, titanium tacks or miniscrews. However, these techniques are marredwith a number of clinical challenges. This article presents the “SauFRa” technique, a novel technique for stabilization of resorbable membranes in both, single as well as multiple implant sites while avoiding the shortcomings of other suturing techniques described in literature. Furthermore, the technique also eliminates the possibility of complications observed when using resorbable pins, titanium tacks or miniscrews, such as damage to adjacent roots during insertion. The authors’ employed this technique in 89 patients (51 male and 35 female) and found no complications like tissue dehiscence, infection or graft migration.

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