Lysosome-targeting pH indicator based on peri-fused naphthalene monoimide with superior stability for long term live cell imaging

2021 ◽  
Vol 9 (1) ◽  
pp. 112-124
Author(s):  
Astrid Tannert ◽  
Javier Garcia Lopez ◽  
Nikolay Petkov ◽  
Anela Ivanova ◽  
Kalina Peneva ◽  
...  
Keyword(s):  
Spectral Properties ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Ph Indicator ◽  
Fluorescent Marker ◽  
Lysosomal Ph ◽  
Ph Changes ◽  
Microscopic Evaluation

Lysosomal pH is altered in many pathophysiological conditions. We describe synthesis and spectral properties of a new lysosomal fluorescent marker dye suitable for microscopic evaluation of lysosomal distribution and pH changes.

Live cell imaging of lysosomal pH changes with pH responsive ratiometric lanthanide probes

2012 ◽  
Vol 48 (68) ◽  
pp. 8520 ◽  
Author(s):  
David G. Smith ◽  
Brian K. McMahon ◽  
Robert Pal ◽  
David Parker
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Ph Responsive ◽  
Lysosomal Ph ◽  
Ph Changes

A novel coumarin derivative as a sensitive probe for tracing intracellular pH changes

RSC Advances ◽  
2015 ◽  
Vol 5 (21) ◽  
pp. 15778-15783 ◽  
Author(s):  
Mengqiang Liu ◽  
Minshan Hu ◽  
Qian Jiang ◽  
Zhiyun Lu ◽  
Yan Huang ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Coumarin Derivative ◽  
Live Cell ◽  
Ph Changes

A novel coumarin derivative was synthesized and its application in live cell imaging was demonstrated.

pH-triggered decomposition of polymeric fluorescent vesicles to induce growth of tetraphenylethylene nanoparticles for long-term live cell imaging

Polymer ◽  
2017 ◽  
Vol 118 ◽  
pp. 75-84 ◽  
Author(s):  
Xing Wang ◽  
Yanyu Yang ◽  
Fei Yang ◽  
Hong Shen ◽  
Decheng Wu
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Long-term fate of etoposide-induced micronuclei and micronucleated cells in Hela-H2B-GFP cells

2020 ◽  
Vol 94 (10) ◽  
pp. 3553-3561
Author(s):  
Hauke Reimann ◽  
Helga Stopper ◽  
Henning Hintzsche
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cellular Structures ◽  
Death Rates ◽  
Daughter Cells

Abstract Micronuclei are small nuclear cellular structures containing whole chromosomes or chromosomal fragments. While there is a lot of information available about the origin and formation of micronuclei, less is known about the fate of micronuclei and micronucleated cells. Possible fates include extrusion, degradation, reincorporation and persistence. Live cell imaging was performed to quantitatively analyse the fates of micronuclei and micronucleated cells occurring in vitro. Imaging was conducted for up to 96 h in HeLa-H2B-GFP cells treated with 0.5, 1 and 2 µg/ml etoposide. While a minority of micronuclei was reincorporated into the main nucleus during mitosis, the majority of micronuclei persisted without any alterations. Degradation and extrusion were observed rarely or never. The presence of micronuclei affected the proliferation of the daughter cells and also had an influence on cell death rates. Mitotic errors were found to be clearly increased in micronucleus-containing cells. The results show that micronuclei and micronucleated cells can, although delayed in cell cycle, sustain for multiple divisions.

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