Unexpected reversible and controllable nuclear uptake and efflux of the DNA “light-switching” Ru(ii)-polypyridyl complex in living cells via ion-pairing with chlorophenolate counter-anions

2020 ◽  
Vol 8 (45) ◽  
pp. 10327-10336
Author(s):  
Rong Huang ◽  
Jian-Qiang Zhu ◽  
Miao Tang ◽  
Chun-Hua Huang ◽  
Zhi-Hui Zhang ◽  
...  
Keyword(s):  
Metal Complexes ◽  
Cellular Uptake ◽  
Ion Pairing ◽  
Living Cells ◽  
Nuclear Uptake ◽  
Polypyridyl Complex

An in-depth understanding of the mechanisms of cellular uptake and efflux would facilitate the design of metal complexes with not only better functionality and targeted theranostic efficiency, but also with controlled toxicity.

scholarly journals Targeted live-cell nuclear delivery of the DNA ‘light-switching’ Ru(II) complex via ion-pairing with chlorophenolate counter-anions: the critical role of binding stability and lipophilicity of the ion-pairing complexes

2019 ◽  
Vol 47 (20) ◽  
pp. 10520-10528 ◽  
Author(s):  
Xi-Juan Chao ◽  
Miao Tang ◽  
Rong Huang ◽  
Chun-Hua Huang ◽  
Jie Shao ◽  
...  
Keyword(s):  
Diffusion Mechanism ◽  
Critical Role ◽  
Ion Pairing ◽  
Chlorinated Phenols ◽  
Cellular Targets ◽  
Nuclear Uptake ◽  
Active Metal ◽  
Physicochemical Factors ◽  
Better Than

Abstract We have found recently that nuclear uptake of the cell-impermeable DNA light-switching Ru(II)-polypyridyl cationic complexes such as [Ru(bpy)2(dppz)]Cl2 was remarkably enhanced by pentachlorophenol (PCP), by forming ion-pairing complexes via a passive diffusion mechanism. However, it is not clear whether the enhanced nuclear uptake of [Ru(bpy)2(dppz)]2+ is only limited to PCP, or it is a general phenomenon for other highly chlorinated phenols (HCPs); and if so, what are the major physicochemical factors in determining nuclear uptake? Here, we found that the nuclear uptake of [Ru(bpy)2(dppz)]2+ can also be facilitated by other two groups of HCPs including three tetrachlorophenol (TeCP) and six trichlorophenol (TCP) isomers. Interestingly and unexpectedly, 2,3,4,5-TeCP was found to be the most effective one for nuclear delivery of [Ru(bpy)2(dppz)]2+, which is even better than the most-highly chlorinated PCP, and much better than its two other TeCP isomers. Further studies showed that the nuclear uptake of [Ru(bpy)2(dppz)]2+ was positively correlated with the binding stability, but to our surprise, inversely correlated with the lipophilicity of the ion-pairing complexes formed between [Ru(bpy)2(dppz)]Cl2 and HCPs. These findings should provide new perspectives for future investigations on using ion-pairing as an effective method for delivering other bio-active metal complexes into their intended cellular targets.

Switchable two-photon imaging of RGD-functionalized polynorbornenes with enhanced cellular uptake in living cells

2016 ◽  
Vol 40 (4) ◽  
pp. 3252-3260 ◽  
Author(s):  
Nan Xie ◽  
Ke Feng ◽  
Bin Chen ◽  
Chen-Ho Tung ◽  
Li-Zhu Wu
Keyword(s):  
Cellular Uptake ◽  
Living Cells ◽  
Rgd Peptides ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

Two-photon imaging polynorbornenes were fabricated directly from photochromic spiropyran, RGD peptides and hydrophilic PEG monomers via modular ROMP.

Differences in cellular transport of tri-iodothyronine and thyroxine: cell cycle-dependent alteration of tri-iodothyronine uptake

1995 ◽  
Vol 147 (3) ◽  
pp. 479-485 ◽  
Author(s):  
T Nagasawa ◽  
K Ichikawa ◽  
K Minemura ◽  
M Hara ◽  
H Yajima ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cellular Uptake ◽  
Sodium Butyrate ◽  
Time Course ◽  
G1 Phase ◽  
Cellular Transport ◽  
Maximal Level ◽  
Maximal Increase ◽  
Nuclear Uptake ◽  
Cycle Dependent

Abstract Cellular and nuclear uptake of tri-iodothyronine (T3) and thyroxine (T4) was examined using the cultured cell line derived from rat liver, clone 9, and rat hepatoma, dRLH-84. The saturable cellular uptake of T3 and T4 was demonstrated in these cells. First we examined the cell cycle-dependent alteration of thyroid hormone uptake. Cellular T3 uptake was minimal in the early G1 phase and increased in the late G1 phase, reaching a maximal level in the S phase. Alterations in nuclear T3 uptake were in accordance with the changes in cellular T3 uptake. On the other hand, cellular and nuclear T4 uptake was unchanged throughout the cell cycle, suggesting the T3 specificity of the cell cycle-dependent alteration of cellular hormone transport. Next we examined the effect of sodium butyrate on the cellular transport of thyroid hormones. After treatment with 5 mm sodium butyrate, cellular and nuclear uptake of T3 was increased, reaching a maximal level (four- to sevenfold increase) after 48 h. When cells were incubated for 48 h with various concentrations of sodium butyrate, T3 uptake was enhanced by 1 mm sodium butyrate, reaching a maximal level with 5 mm. Although cellular T4 uptake was also increased after treatment with sodium butyrate, the degree and time-course of the increase were different from those of T3. The maximal increase in cellular T4 uptake (two- to threefold increase) was attained 20 h after treatment. Despite the increase in cellular T4 uptake, nuclear T4 uptake was decreased after treatment with sodium butyrate. For both T3 and T4, the enhanced cellular uptake was due to the increased Vmax without changes in the Michaelis–Menten constant. These data indicate that cellular transport of T4 is different from that of T3 in rat hepatic cells. Journal of Endocrinology (1995) 147, 479–485

scholarly journals Monitoring the Kinetics of the Cellular Uptake of a Metal Carbonyl Conjugated with a Lipidic Moiety in Living Cells Using Synchrotron Infrared Spectromicroscopy

2019 ◽  
Vol 74 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Sylvain Clède ◽  
Christophe Sandt ◽  
Paul Dumas ◽  
Clotilde Policar
Keyword(s):  
Breast Cancer ◽  
Cellular Uptake ◽  
Microfluidic Device ◽  
Metal Carbonyl ◽  
Living Cells ◽  
High Survival ◽  
High Survival Rate ◽  
Short Term ◽  
Kinetics Of ◽  
Single Living

Presented here is the exploitation of synchrotron infrared spectromicroscopy to evaluate the feasibility of monitoring the cellular uptake of rhenium-tris-carbonyl-tagged (Re(CO)3) lipophilic chains in living cells. To this aim, an in-house thermostated microfluidic device was used to limit water absorption while keeping cells alive. Indeed, cells showed a high survival rate in the microfluidic device over the course of the experiment, proving the short-term biocompatibility of the device. We recorded spectra of single, living, fully hydrated breast cancer MDA-MB231 cells and could follow the penetration of the rhenium complexes for up to 2 h. Despite the strong variations observed in the uptake kinetics between individual cells, the Re(CO)3 complex was traced inside the cells at low concentration and shown to enter them on the hour time scale by active transport.

Synthesis and strong photooxidation power of a supramolecular hybrid comprising a polyoxometalate and Ru(ii) polypyridyl complex with zinc(ii)

2015 ◽  
Vol 185 ◽  
pp. 171-185 ◽  
Author(s):  
Kenji Ohashi ◽  
Hiroyuki Takeda ◽  
Kazuhide Koike ◽  
Osamu Ishitani
Keyword(s):  
Quantum Yield ◽  
Metal Complex ◽  
Metal Complexes ◽  
Keggin Type ◽  
Polypyridyl Complex ◽  
Rapid Accumulation ◽  
Novel Method ◽  
The One

A novel method for constructing supramolecular hybrids composed of polyoxometalates and photofunctional metal complexes was developed. A Ru(ii) complex with phosphonate groups (RuP) strongly interacted with Zn(ii) to afford a 2 : 1 trinuclear metal complex ([(RuP)2Zn]3+). In dimethylsulfoxide, [(RuP)2Zn]3+ strongly interacted with a Keggin-type heteropolyoxometalate (Si-WPOM) to form a 1 : 1 hybrid ([(RuP)2Zn]-POM). Irradiation of [(RuP)2Zn]-POM in the presence of diethanolamine caused rapid accumulation of the one-electron reduced hybrid with a quantum yield of 0.99.

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