scholarly journals Enhanced photodynamic therapy and fluorescence imaging using gold nanorods for porphyrin delivery in a novel in vitro squamous cell carcinoma 3D model

2020 ◽  
Vol 8 (23) ◽  
pp. 5131-5142
Author(s):  
Fatma Demir Duman ◽  
Matej Sebek ◽  
Nguyễn T. K. Thanh ◽  
Marilena Loizidou ◽  
Kaveh Shakib ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Photodynamic Therapy ◽  
Cell Carcinoma ◽  
Fluorescence Imaging ◽  
Squamous Cell ◽  
Gold Nanorods ◽  
3D Model ◽  
Cationic Porphyrin ◽  
Monolayer Cultures

In this paper, we show enhanced photodynamic therapy and fluorescence imaging using cationic porphyrin photosensitizer TMPyP loaded gold nanorods in 2D monolayer cultures and a novel in vitro head and neck squamous cell carcinoma 3D model.

Thermally activated delayed fluorescent photosensitizer for photodynamic therapy of oral squamous cell carcinoma under low laser intensity

2021 ◽  
Author(s):  
Shiqi Hu ◽  
Bin Huang ◽  
Yumei Pu ◽  
Chengwan Xia ◽  
Qian Zhang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Photodynamic Therapy ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Laser Intensity ◽  
Thermally Activated

In this report, a new Thermally activated delayed fluorescent(TADF) molecule [2-(4-triphenylvinyl-phenyl)-anthraquinone (TPE-AQ)] was synthesized. This nanomaterial has satisfactory photostability. In vitro, it was indicated that these TADF nanoparticles (NPs) were...

scholarly journals An in vitro study of the effect of 5-ALA-mediated photodynamic therapy on oral squamous cell carcinoma

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ying Ma ◽  
Shujuan Qu ◽  
Liangpeng Xu ◽  
Hongbo Lu ◽  
Baoguo Li
Keyword(s):  
Squamous Cell Carcinoma ◽  
Photodynamic Therapy ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Laser Irradiation ◽  
Squamous Cell ◽  
Protoporphyrin Ix ◽  
Control Group ◽  
Blank Control Group

Abstract Background The primary aim of this study was to observe the effect of 5-ALA-mediated photodynamic therapy on oral squamous cell carcinoma in vitro. Methods SCC25 cells were divided into the observation group and the blank control group. Different concentrations of 5-ALA and SCC25 cells were co-incubated for different times, and the concentration of protoporphyrin IX was detected by flow cytometry. SCC25 cells were divided into the 5-ALA group (100 mg/L), the laser irradiation group alone, the 5-ALA plus laser irradiation group, and the blank control group (0 mg/L 5-ALA), and the methyl thiazolyl tetrazolium (MTT) solution method was used (each group was incubated for 4, 8 and 12 h in turn). The cell survival rate was calculated. Using annexin V-fluorescein isothiocyanate/propidium iodide method, the apoptosis of SCC25 cells was detected by flow cytometry. Results The level of protoporphyrin IX in SCC25 cells increased with increased concentrations of 5-ALA and length of incubation. However, after 12 h, protoporphyrin IX level in SCC25 cells was gradually stabilized, and similar effect was obtained with 100 mg/L or more 5-ALA, indicating that the level of protoporphyrin IX in SCC25 cells was determined by 5-ALA concentration and incubation time. 5-ALA plus laser irradiation exerted an inhibitory effect on the growth of SCC25 cells, which was highly associated with drug dose and incubation time. Compared with the control group, laser irradiation alone or 5-ALA alone had no effect on the apoptosis of SCC25 cells. Different concentrations of 5-ALA combined with laser irradiation showed a remarkable effect of apoptosis, and a higher apoptosis rate was seen with higher drug concentrations. Conclusion 5-ALA-mediated photodynamic therapy affects the growth of SCC25 cells in vitro, which may provide a new idea for the clinical treatment of oral squamous cell carcinoma.

An in vitro 3D-Model of Invasive Squamous Cell Carcinoma (SCC) Recapitulates in vivo Pathology

2003 ◽  
Vol 104 (s49) ◽  
pp. 28P-29P
Author(s):  
ML Nystrom ◽  
GJ Thomas ◽  
IC MacKenzie ◽  
IR Hart ◽  
JF Marshall
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
3D Model ◽  
Invasive Squamous Cell Carcinoma

scholarly journals Photodynamic Therapy of Human Squamous Cell Carcinoma In Vitro and in Xenografts in Nude Mice

1993 ◽  
Vol 103 (9) ◽  
pp. 967???975 ◽  
Author(s):  
Cliff A. Megerian ◽  
Syed I. A. Zaidi ◽  
Robert C. Sprecher ◽  
Sebouh Setrakian ◽  
David W. Stepnick ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Photodynamic Therapy ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Nude Mice ◽  
Human Squamous Cell Carcinoma
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