High-aspect-ratio water-dispersed gold nanowires incorporated within gelatin methacrylate hydrogels for constructing cardiac tissues in vitro

2020 ◽  
Vol 8 (32) ◽  
pp. 7213-7224 ◽  
Author(s):  
Xiao-Pei Li ◽  
Kai-Yun Qu ◽  
Feng Zhang ◽  
Han-Ning Jiang ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Cardiac Tissue ◽  
Gold Nanowires ◽  
Tissue Formation ◽  
Cardiac Tissues ◽  
Cardiomyocyte Culture ◽  
Dispersed Gold

The prepared high-aspect-ratio water-dispersed gold nanowires are incorporated into GeIMA hydrogels for cardiomyocyte culture and micro-cardiac tissue formation.

scholarly journals Correction: High-aspect-ratio water-dispersed gold nanowires incorporated within gelatin methacrylate hydrogels for constructing cardiac tissues in vitro

2020 ◽  
Author(s):  
Xiao-Pei Li ◽  
Kai-Yun Qu ◽  
Feng Zhang ◽  
Han-Ning Jiang ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Gold Nanowires ◽  
Cardiac Tissues ◽  
Dispersed Gold

Correction for ‘High-aspect-ratio water-dispersed gold nanowires incorporated within gelatin methacrylate hydrogels for constructing cardiac tissues in vitro’ by Xiao-Pei Li et al., J. Mater. Chem. B, 2020, 8, 7213–7224, DOI: 10.1039/D0TB00768D.

Fabrication of high-aspect-ratio microstructures in polymer microfluid chips for in vitro single-cell analysis

2016 ◽  
Vol 61 (10) ◽  
pp. 1566-1571 ◽  
Author(s):  
A. S. Bukatin ◽  
I. S. Mukhin ◽  
E. I. Malyshev ◽  
I. V. Kukhtevich ◽  
A. A. Evstrapov ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
Single Cell ◽  
High Aspect Ratio ◽  
Single Cell Analysis ◽  
Cell Analysis

Biophysical stimulation for in vitro engineering of functional cardiac tissues

2017 ◽  
Vol 131 (13) ◽  
pp. 1393-1404 ◽  
Author(s):  
Anastasia Korolj ◽  
Erika Yan Wang ◽  
Robert A. Civitarese ◽  
Milica Radisic
Keyword(s):  
Cardiac Tissue ◽  
Culture Media ◽  
Culture Conditions ◽  
Lineage Specification ◽  
Cardiac Tissues ◽  
Cardiac Lineage ◽  
And Function ◽  
Tissue Models

Engineering functional cardiac tissues remains an ongoing significant challenge due to the complexity of the native environment. However, our growing understanding of key parameters of the in vivo cardiac microenvironment and our ability to replicate those parameters in vitro are resulting in the development of increasingly sophisticated models of engineered cardiac tissues (ECT). This review examines some of the most relevant parameters that may be applied in culture leading to higher fidelity cardiac tissue models. These include the biochemical composition of culture media and cardiac lineage specification, co-culture conditions, electrical and mechanical stimulation, and the application of hydrogels, various biomaterials, and scaffolds. The review will also summarize some of the recent functional human tissue models that have been developed for in vivo and in vitro applications. Ultimately, the creation of sophisticated ECT that replicate native structure and function will be instrumental in advancing cell-based therapeutics and in providing advanced models for drug discovery and testing.

Abstract 30: In vitro Fabrication of Human Cardiac Tissues With Porcine Perfusable Blood Vessels

2016 ◽  
Vol 119 (suppl_1) ◽  
Author(s):  
Akitoshi Inui ◽  
Hidekazu Sekine ◽  
Kazunori Sano ◽  
Izumi Dobashi ◽  
Azumi Yoshida ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Cardiac Tissue ◽  
Severe Heart Failure ◽  
Perfusion Culture ◽  
Cardiac Cell ◽  
Cell Sheets ◽  
Cardiac Tissues ◽  
Bioreactor System ◽  
Human Cardiac Tissue

The definitive treatment of severe heart failure is heart transplantation; however the number of heart transplantation procedures performed in Japan per year ranges from 30-40 due to donor shortage. Therefore, recently other treatments such as ventricular assist device or regenerative therapy by human cardiac tissue engineering have been developed and are considered as appropriate alternatives. We have developed an original technology, which was named cell-sheet based tissue engineering to fabricate functional three-dimensional tissue by layering cell sheets. The utilization of this technique allowed us to successfully engineer thick rat cardiac tissue with perfusable blood vessels in vitro. Here, we demonstrate a technique to engineer human cardiac tissue with perfusable blood vessels using cardiac cell sheets derived from human induced pluripotent stem cells, and porcine small intestine as a vascular bed for perfusion culture. The small intestine was harvested from with a branch of the superior mesenteric artery and vein and underwent mucosal resection after harvested tissue was cut open. To engineer cardiac tissue with perfusable blood vessels, cardiac cell sheets co-cultured with endothelial cells, were triple-layered and then was overlaid on the vascular bed in the bioreactor system. One day after perfusion culture, overlaid cardiac tissues pulsated spontaneously and were synchronized. The cardiac tissue construct was viable tissue without any observable necrosis. Furthermore we examined the possibility of transplantation of the in vitro engineered human cardiac tissue with the connectable host artery and vein. Engineered cardiac tissue was removed from the bioreactor system after 4-day perfusion, and transplanted to another pig heart. The branch of the superior mesenteric artery and vein of the graft were then reconnected to the host internal thoracic artery and vein. When the cardiac tissue reperfused, it began to beat spontaneously after a few minutes. We believe that this method is useful to fabricate functional cardiac tissue and may become an appropriate treatment for severe heart failure.

scholarly journals Far-field midinfrared superresolution imaging and spectroscopy of single high aspect ratio gold nanowires

2020 ◽  
Vol 117 (5) ◽  
pp. 2288-2293 ◽  
Author(s):  
Kyle Aleshire ◽  
Ilia M. Pavlovetc ◽  
Robyn Collette ◽  
Xiang-Tian Kong ◽  
Philip D. Rack ◽  
...  
Keyword(s):  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Optical Measurement ◽  
Near Field ◽  
Metal Nanostructures ◽  
Gold Nanowires ◽  
Far Field ◽  
Ambient Conditions ◽  
Fabry Perot ◽  
Imaging And Spectroscopy

Limited approaches exist for imaging and recording spectra of individual nanostructures in the midinfrared region. Here we use infrared photothermal heterodyne imaging (IR-PHI) to interrogate single, high aspect ratio Au nanowires (NWs). Spectra recorded between 2,800 and 4,000 cm−1 for 2.5–3.9-μm-long NWs reveal a series of resonances due to the Fabry–Pérot modes of the NWs. Crucially, IR-PHI images show structure that reflects the spatial distribution of the NW absorption, and allow the resonances to be assigned to the m = 3 and m = 4 Fabry–Pérot modes. This far-field optical measurement has been used to image the mode structure of plasmon resonances in metal nanostructures, and is made possible by the superresolution capabilities of IR-PHI. The linewidths in the NW spectra range from 35 to 75 meV and, in several cases, are significantly below the limiting values predicted by the bulk Au Drude damping parameter. These linewidths imply long dephasing times, and are attributed to reduction in both radiation damping and resistive heating effects in the NWs. Compared to previous imaging studies of NW Fabry–Pérot modes using electron microscopy or near-field optical scanning techniques, IR-PHI experiments are performed under ambient conditions, enabling detailed studies of how the environment affects mid-IR plasmons.

Particokinetics and in vitro dose of high aspect ratio nanoparticles

Nanoscale ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 5209-5214 ◽  
Author(s):  
Seth Richard Price ◽  
Calum Kinnear ◽  
Sandor Balog
Keyword(s):  
Aspect Ratio ◽  
Cell Cultures ◽  
High Aspect Ratio ◽  
Adherent Cell

Adapting computational particokinetic models to address the dosage of high-aspect ratio nanomaterials for in vitro nanoparticle toxicology assays involving submerged adherent cell cultures.

scholarly journals Chronic optical pacing conditioning of h-iPSC engineered cardiac tissues

2019 ◽  
Vol 10 ◽  
pp. 204173141984174 ◽  
Author(s):  
Marc Dwenger ◽  
William J Kowalski ◽  
Fei Ye ◽  
Fangping Yuan ◽  
Joseph P Tinney ◽  
...  
Keyword(s):  
Stem Cell ◽  
Electrical Stimulation ◽  
Pluripotent Stem Cell ◽  
Cardiac Tissue ◽  
Induced Pluripotent Stem Cell ◽  
Mechanical Stretch ◽  
In Vitro Models ◽  
Cardiac Tissues ◽  
Induced Pluripotent

The immaturity of human induced pluripotent stem cell derived engineered cardiac tissues limits their ability to regenerate damaged myocardium and to serve as robust in vitro models for human disease and drug toxicity studies. Several chronic biomimetic conditioning protocols, including mechanical stretch, perfusion, and/or electrical stimulation promote engineered cardiac tissue maturation but have significant technical limitations. Non-contacting chronic optical stimulation using heterologously expressed channelrhodopsin light-gated ion channels, termed optogenetics, may be an advantageous alternative to chronic invasive electrical stimulation for engineered cardiac tissue conditioning. We designed proof-of-principle experiments to successfully transfect human induced pluripotent stem cell derived engineered cardiac tissues with a desensitization resistant, chimeric channelrhodopsin protein, and then optically paced engineered cardiac tissues to accelerate maturation. We transfected human induced pluripotent stem cell engineered cardiac tissues using an adeno-associated virus packaged chimeric channelrhodopsin and then verified optically paced by whole cell patch clamp. Engineered cardiac tissues were then chronically optically paced above their intrinsic beat rates in vitro from day 7 to 14. Chronically optically paced resulted in improved engineered cardiac tissue electrophysiological properties and subtle changes in the expression of some cardiac relevant genes, though active force generation and histology were unchanged. These results validate the feasibility of a novel chronically optically paced paradigm to explore non-invasive and scalable optically paced–induced engineered cardiac tissue maturation strategies.

scholarly journals Cardiac tissue engineering: current state-of-the-art materials, cells and tissue formation

2018 ◽  
Vol 16 (3) ◽  
Author(s):  
Isabella Caroline Pereira Rodrigues ◽  
Andreas Kaasi ◽  
Rubens Maciel Filho ◽  
André Luiz Jardini ◽  
Laís Pellizzer Gabriel
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Material Selection ◽  
Cardiac Tissue Engineering ◽  
Tissue Formation ◽  
Scaffold Material ◽  
Interdisciplinary Field ◽  
Current State ◽  
Whole Heart

ABSTRACT Cardiovascular diseases are the major cause of death worldwide. The heart has limited capacity of regeneration, therefore, transplantation is the only solution in some cases despite presenting many disadvantages. Tissue engineering has been considered the ideal strategy for regenerative medicine in cardiology. It is an interdisciplinary field combining many techniques that aim to maintain, regenerate or replace a tissue or organ. The main approach of cardiac tissue engineering is to create cardiac grafts, either whole heart substitutes or tissues that can be efficiently implanted in the organism, regenerating the tissue and giving rise to a fully functional heart, without causing side effects, such as immunogenicity. In this review, we systematically present and compare the techniques that have drawn the most attention in this field and that generally have focused on four important issues: the scaffold material selection, the scaffold material production, cellular selection and in vitro cell culture. Many studies used several techniques that are herein presented, including biopolymers, decellularization and bioreactors, and made significant advances, either seeking a graft or an entire bioartificial heart. However, much work remains to better understand and improve existing techniques, to develop robust, efficient and efficacious methods.

scholarly journals PVP Assisted Synthesis of Hydroxyapatite Nanorods with Tunable Aspect Ratio and Bioactivity

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
A. Joseph Nathanael ◽  
Young Ho Seo ◽  
Tae Hwan Oh
Keyword(s):  
Electron Microscopy ◽  
Aspect Ratio ◽  
High Aspect Ratio ◽  
Xrd Analysis ◽  
Reaction Model ◽  
Morphological Variations ◽  
Viability Analysis ◽  
Selected Area Electron Diffraction ◽  
Length Width

Highly crystalline and reasonably uniform hydroxyapatite (HA) nanorods were prepared by polyvinylpyrrolidone (PVP) assisted hydrothermal synthesis which produces high aspect ratio (length/width) nanorods. The aspect ratio of the nanorods was higher in the presence of PVP and increased with increasing concentrations of PVP. X-ray diffraction (XRD) analysis showed that the HA nanorods were of the hexagonal apatite phase. High resolution transmission electron microscopy (HRTEM) and selected area electron diffraction (SAED) patterns confirmed that the nanorods grew in thec-axis direction (preferred orientation). The calculated lattice spacing was ~0.35 nm which is thec-axis value of hexagonal HA. Morphological variations of pristine and PVP added HA were evaluated by field emission scanning electron microscopy (FESEM) and TEM, which revealed that the presence of PVP greatly increased the aspect ratio of the HA nanorods. The formation mechanism of the PVP assisted HA was studied and a possible reaction model was given. Cell viability analysis byin vitrostudies showed encouraging results for the high aspect ratio nanorods and indicated a possibility for tuning the activity based on controlling the aspect ratio.

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