3D hollow-out TiO2 nanowire cluster/GOx as an ultrasensitive photoelectrochemical glucose biosensor

2020 ◽  
Vol 8 (11) ◽  
pp. 2363-2370 ◽  
Author(s):  
Wenke Yang ◽  
Xiaohong Wang ◽  
Wanjun Hao ◽  
Qiang Wu ◽  
Juan Peng ◽  
...  
Keyword(s):  
Active Sites ◽  
Glucose Biosensor ◽  
Diffusion Effect ◽  
Enhanced Diffusion ◽  
Tio2 Nanowire ◽  
Higher Sensitivity ◽  
Sensitivity Performance

A reticulate TiO2 nanowire cluster electrode demonstrated higher sensitivity performance due to enhanced diffusion effect and more number of active sites.

Electrochemical MIP-Sensors for Drugs

2018 ◽  
Vol 25 (33) ◽  
pp. 4007-4019 ◽  
Author(s):  
Aysu Yarman ◽  
Sevinc Kurbanoglu ◽  
Katharina J. Jetzschmann ◽  
Sibel A. Ozkan ◽  
Ulla Wollenberger ◽  
...  
Keyword(s):  
Active Sites ◽  
Quartz Crystal ◽  
Differential Pulse ◽  
Imprinted Polymer ◽  
Template Removal ◽  
Functional Monomers ◽  
Synthetic Materials ◽  
Electrochemical Devices ◽  
Pulse Voltammetry ◽  
Higher Sensitivity

In order to replace bio-macromolecules by stable synthetic materials in separation techniques and bioanalysis biomimetic receptors and catalysts have been developed: Functional monomers are polymerized together with the target analyte and after template removal cavities are formed in the ”molecularly imprinted polymer” (MIP) which resemble the active sites of antibodies and enzymes. Starting almost 80 years ago, around 1,100 papers on MIPs were published in 2016. Electropolymerization allows to deposit MIPs directly on voltammetric electrodes or chips for quartz crystal microbalance (QCM) and surface plasmon resonance (SPR). For the readout of MIPs for drugs amperometry, differential pulse voltammetry (DPV) and impedance spectroscopy (EIS) offer higher sensitivity as compared with QCM or SPR. Application of simple electrochemical devices allows both the reproducible preparation of MIP sensors, but also the sensitive signal generation. Electrochemical MIP-sensors for the whole arsenal of drugs, e.g. the most frequently used analgesics, antibiotics and anticancer drugs have been presented in literature and tested under laboratory conditions. These biomimetic sensors typically have measuring ranges covering the lower nano- up to millimolar concentration range and they are stable under extreme pH and in organic solvents like nonaqueous extracts.

Ni(OH)2/NiO nanosheet with opulent active sites for high-performance glucose biosensor

2017 ◽  
Vol 248 ◽  
pp. 169-177 ◽  
Author(s):  
Wei Huang ◽  
Lianyuan Ge ◽  
Yong Chen ◽  
Xiaoyong Lai ◽  
Juan Peng ◽  
...  
Keyword(s):  
Active Sites ◽  
High Performance ◽  
Glucose Biosensor

Anomalous Diffusion in Membranes

1998 ◽  
Vol 543 ◽  
Author(s):  
R. Granek ◽  
S. Pierrat ◽  
A. G. Zilman
Keyword(s):  
Anomalous Diffusion ◽  
Active Sites ◽  
The Other ◽  
Chemical Energy ◽  
Enhanced Diffusion ◽  
Transverse Diffusion

AbstractWe study the undulations and the transverse diffusion of a tagged membrane point in both physical (passive) membranes and active biomembranes. In physical membranes thermal undulations generate a transverse subdiffusive motion, 〈r2〉 ∼ t2/3. Active biomembranes include active sites that use chemical energy to pump ions or molecules from one side to the other. In this case we find a few regimes which show a strongly enhanced diffusion, 〈r2〉 ∼ tα with 1 < α > 2.

scholarly journals A Molecular Interaction Analysis Reveals the Possible Roles of Graphene Oxide in a Glucose Biosensor

Biosensors ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 18 ◽  
Author(s):  
Tony Sumaryada ◽  
Muhammad Sandy Gunawan ◽  
Salahuddin Perdana ◽  
Sugianto Arjo ◽  
Akhiruddin Maddu
Keyword(s):  
Graphene Oxide ◽  
Molecular Docking ◽  
Binding Affinity ◽  
Active Sites ◽  
Interaction Analysis ◽  
Glucose Sensing ◽  
Glucose Biosensor ◽  
Oxide Surface ◽  
Sensing Mechanism ◽  
Biosensor System

In this paper, we report the molecular docking study of graphene oxide and glucose oxidase (GOx) enzyme for a potential glucose biosensing application. The large surface area and good electrical properties have made graphene oxide as one of the best candidates for an enzyme immobilizer and transducer in the biosensing system. Our molecular docking results revealed that graphene oxide plays a role as a GOx enzyme immobilizer in the glucose biosensor system since it can spontaneously bind with GOx at specific regions separated from the active sites of glucose and not interfering or blocking the glucose sensing by GOx in an enzyme-assisted biosensor system. The strongest binding affinity of GOx-graphene oxide interaction is −11.6 kCal/mol and dominated by hydrophobic interaction. Other modes of interactions with a lower binding affinity have shown the existence of some hydrogen bonds (H-bonds). A possibility of direct sensing (interaction) model of glucose by graphene oxide (non-enzymatic sensing mechanism) was also studied in this paper, and showed a possible direct glucose sensing by graphene oxide through the H-bond interaction, even though with a much lower binding affinity of −4.2 kCal/mol. It was also found that in a direct glucose sensing mechanism, the sensing interaction can take place anywhere on the graphene oxide surface with almost similar binding affinity.

scholarly journals Highly Sensitive Voltammetric Glucose Biosensor Based on Glucose Oxidase Encapsulated in a Chitosan/Kappa-Carrageenan/Gold Nanoparticle Bionanocomposite

2018 ◽  
Author(s):  
Nicole Jaffrezic-Renault ◽  
Ilhem Rassas ◽  
Mohamed Braiek ◽  
Anne Bonhomme ◽  
François Bessueille ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Modified Electrode ◽  
Gold Electrode ◽  
Polyelectrolyte Complex ◽  
Glucose Sensing ◽  
Glucose Biosensor ◽  
Good Reproducibility ◽  
Highly Sensitive ◽  
Kappa Carrageenan ◽  
Higher Sensitivity

In this work, an enzymatic sensor, based on a bionanocomposite film consisting of a polyelectrolyte complex (PEC) [Chitosan/kappa-carrageenan] doped with gold nanoparticles (AuNPs) encapsulating glucose oxidase (GOD) deposited on a gold electrode (Au) for glucose sensing, is described. Using the electrocatalytic synergy of AuNPs and GOD as a model of enzyme, the variation of the current (&micro;A) as a function of the log of the glucose concentration (log [glucose]), shows 3 times higher sensitivity for the modified electrode (283.9) compared to that of the PEC/GOD modified electrode (93.7), with a detection limit of about 5 &micro;M and a linearity range between 10&micro;M and 7mM. The response of the PEC/AuNPs/GOD based biosensor also presents good reproducibility, stability and negligible interfering effects from ascorbic acid, uric acid, urea and creatinine. The applicability of the PEC/AuNPs/GOD based biosensor was tested in glucose-spiked saliva samples and acceptable recovery rates were obtained.

scholarly journals Highly Sensitive Voltammetric Glucose Biosensor Based on Glucose Oxidase Encapsulated in a Chitosan/Kappa-Carrageenan/Gold Nanoparticle Bionanocomposite

Sensors ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 154 ◽  
Author(s):  
Ilhem Rassas ◽  
Mohamed Braiek ◽  
Anne Bonhomme ◽  
Francois Bessueille ◽  
Guy Raffin ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Modified Electrode ◽  
Gold Electrode ◽  
Polyelectrolyte Complex ◽  
Glucose Sensing ◽  
Glucose Biosensor ◽  
Good Reproducibility ◽  
Highly Sensitive ◽  
Kappa Carrageenan ◽  
Higher Sensitivity

In this work, an enzymatic sensor, based on a bionanocomposite film consisting of a polyelectrolyte complex (PEC) (Chitosan/kappa-carrageenan) doped with gold nanoparticles (AuNPs) encapsulating glucose oxidase (GOD) deposited on a gold electrode (Au) for glucose sensing, is described. Using the electrocatalytic synergy of AuNPs and GOD as a model of enzyme, the variation of the current (µA) as a function of the log of the glucose concentration (log [glucose]), shows three times higher sensitivity for the modified electrode (283.9) compared to that of the PEC/GOD modified electrode (93.7), with a detection limit of about 5 µM and a linearity range between 10 µM and 7 mM. The response of the PEC/AuNPs/GOD based biosensor also presents good reproducibility, stability, and negligible interfering effects from ascorbic acid, uric acid, urea, and creatinine. The applicability of the PEC/AuNPs/GOD based biosensor was tested in glucose-spiked saliva samples and acceptable recovery rates were obtained.

ZnO Nanonails: Synthesis and Their Application as Glucose Biosensor

2008 ◽  
Vol 8 (6) ◽  
pp. 3216-3221 ◽  
Author(s):  
Ahmad Umar ◽  
M. M. Rahman ◽  
S. H. Kim ◽  
Y. B. Hahn
Keyword(s):  
Electron Transfer ◽  
Zinc Oxide ◽  
Detection Limit ◽  
Electrode Surface ◽  
Active Sites ◽  
Thermal Evaporation ◽  
Direct Electron Transfer ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Evaporation Process

Well-crystallized zinc oxide nanonails were grown in a high density by thermal evaporation process and were used as supporting matrixes for glucose oxidase (GOx) immobilization to construct efficient glucose biosensor. The GOx attached to the surfaces of ZnO nanonails had more spatial freedom in its orientation, which facilitated the direct electron transfer between the active sites of immobilized GOx and electrode surface. The fabricated biosensor showed a high sensitivity of 24.613 μA cm–2 mM–1 with a response time less than 10 s. Moreover, it shows a linear range from 0.1 to 7.1 mM with a correlation coefficient of R = 0.9937 and detection limit of 5 μM.

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