scholarly journals Poroelastic shape relaxation of hydrogel particles

Soft Matter ◽  
2021 ◽  
Author(s):  
Jean-François Louf ◽  
Sujit S. Datta
Keyword(s):  
Diffusion Coefficient ◽  
Granular Packing ◽  
Hydrogel Particles ◽  
Hydrogel Particle

We describe a straightforward way to determine the poroelastic diffusion coefficient of a hydrogel particle by first indenting it via swelling in a granular packing, and then monitoring how its indented shape relaxes after it is removed.

scholarly journals Counting of Enzymatically Amplified Affinity Reactions in Hydrogel Particle-Templated Drops

2021 ◽  
Author(s):  
Yilian Wang ◽  
Vishwesh Shah ◽  
Angela Lu ◽  
Ella Pachler ◽  
Brian Cheng ◽  
...  
Keyword(s):  
Dynamic Range ◽  
High Sensitivity ◽  
Microfluidic Devices ◽  
Imaging Systems ◽  
Enzymatic Reactions ◽  
Hydrogel Particles ◽  
Hydrogel Surface ◽  
Hydrogel Particle ◽  
Particle Solution ◽  
Digital Counting

Counting of numerous compartmentalized enzymatic reactions underlies quantitative and high sensitivity immunodiagnostic assays. However, digital enzyme-linked immunosorbent assays (ELISA) require specialized instruments which have slowed adoption in research and clinical labs. We present a lab-on-a-particle solution to digital counting of thousands of single enzymatic reactions. Hydrogel particles are used to bind enzymes and template the formation of droplets that compartmentalize reactions with simple pipetting steps. These hydrogel particles can be made at a high throughput, stored, and used during the assay to create ~500,000 compartments within 2 minutes. These particles can also be dried and rehydrated with sample, amplifying the sensitivity of the assay by driving affinity interactions on the hydrogel surface. We demonstrate digital counting of β-galactosidase enzyme at a femtomolar detection limit with a dynamic range of 3 orders of magnitude using standard benchtop equipment and experiment techniques. This approach can faciliate the development of digital ELISAs with reduced need for specialized microfluidic devices, instruments, or imaging systems.

Investigating the Process of Producing Composite Hydrogel Particle Slurry

2020 ◽  
pp. 123-137
Author(s):  
D.A. Makarenkov ◽  
E.M. Fedorova ◽  
V.A. Buchryakova ◽  
S.L. Lobastov ◽  
V.I. Nazarov ◽  
...  
Keyword(s):  
Particle Size ◽  
Particle Size Distribution ◽  
Size Distribution ◽  
Composite Hydrogel ◽  
Narrow Particle Size Distribution ◽  
Hydrogel Particles ◽  
Multi Stage ◽  
Hydrogel Particle ◽  
Anti Stokes ◽  
Aqueous Slurry

The paper presents comprehensive investigation results concerning obtaining a composite hydrogel material in the form of an aqueous slurry. We considered the issue of grinding hydrogel in water so as to obtain an aqueous slurry of hydrogel particles featuring the desired particle size distribution. We selected polyacrylamide to be the composite hydrogel material and used an anti-Stokes phosphor as a filler. We describe a multi-stage method for producing hydrogel, a coarse grinding process for hydrogel blocks, and a fine grinding technology employing an immersion disperser. We obtained slurries featuring a narrow particle size distribution (160--1000 µm). We provide data on investigating how the manufacturing process parameters and modal parameters of the equipment used affect the particle size distribution in the resulting hydrogel material. We propose a technology for producing composite hydrogel particle slurry in a single process cycle

Brief-Measurements of Vapor Diffusion Coefficient

1954 ◽  
Vol 46 (11) ◽  
pp. 47-49 ◽  
Author(s):  
C.Y. Lee ◽  
C.R. Wilke
Keyword(s):  
Diffusion Coefficient ◽  
Vapor Diffusion
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