Interaction of Proteins with Phase Boundaries in Aqueous Two-Phase Systems under Electric Fields

Soft Matter ◽  
2021 ◽  
Author(s):  
Florian Gebhard ◽  
Johannes Hartmann ◽  
Steffen Hardt
Keyword(s):  
Polyethylene Glycol ◽  
Fluorescence Microscopy ◽  
Electric Field ◽  
Electric Fields ◽  
Microfluidic Device ◽  
Phase System ◽  
Phase Boundaries ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

The electric-field driven transport of proteins across the liquid-liquid interface in an aqueous two-phase system (ATPS) is studied in a microfluidic device using fluorescence microscopy. An ATPS containing polyethylene glycol...

scholarly journals Protein Partitioning In A Droplet-Based Aqueous-Two Phase System Microfluidic Device

2021 ◽  
Author(s):  
David Hernández Cid ◽  
Roberto Carlos Gallo-Villanueva ◽  
José González-Valdez ◽  
Victor Hugo Pérez González ◽  
Marco Arnulfo Mata-Gómez
Keyword(s):  
Microfluidic Device ◽  
Rnase A ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Biological Entities ◽  
Phosphate Salts ◽  
Pegylated Form

Abstract Aqueous-Two Phase Systems (ATPS) is an important tool for the separation of biological entities as proteins, membranes, enzymes, among others. On the other hand, microfluidics is an emerging technology that studies and manipulates liquids either one single phase or dispersed fluids such as droplets at the micro or smaller scales. Applications of microfluidics in different areas such as molecular biology, biochemical analysis and bioprocess have increased in the last years. In this work, we proposed a droplet-based microfluidic approach to generate ATPS systems and to observe how two model proteins, native ribonuclease A (RNase A) and its PEGylated form (PEG-RNase A), behave and partition on these systems. Using polyethylenglycol (PEG) and potasium phosphate salts as the phase-forming chemicals, we were able to form ATPS systems inside the microfluidic device as commonly performed in conventional ATPS macrosystems. Even more, formation of ATPS systems in which one of the fluids was present as a droplet was also achieved. As expected, model proteins exhibited the same behavior as they do in a macrosystem, that is, they displaced to a particular phase according to their affinity for them. When native RNase A was placed in the salt-rich phase, it remained there, and migrated from the PEG-rich phase to the former. On its part, PEGylated RNase A remained in the PEGrich phase or migrated from salt-rich phase to the PEG-rich phase. These results open the possibility for a prospect of micro bioprocess to separate interest biomolecules.

Isolation of Myxosoma cerebralis (Whirling Disease) Spores from Infected Fish by use of a Physical Separation Technique

1980 ◽  
Vol 37 (6) ◽  
pp. 1032-1035 ◽  
Author(s):  
Thomas R. Kozel ◽  
Marley Lott ◽  
Robert Taylor
Keyword(s):  
Polyethylene Glycol ◽  
Fish Tissue ◽  
Immiscible Liquid ◽  
Infected Fish ◽  
Phase System ◽  
Two Phase ◽  
Lower Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Highly Hydrophobic

Immiscible, liquid, two-phase systems are produced when aqueous solutions of dextran and of polyethylene glycol are mixed above certain concentrations. This phase system was used to isolate Myxosoma cerebralis spores from infected fish tissue. Myxosoma cerebralis partitioned into the polyethylene-glycol-rich upper phase, whereas the tissue debris partitioned into the dextran-rich lower phase. Myxosoma cerebralis spores partitioned into the upper phase, regardless of the electrostatic potential in the phase system, indicating that isolation of M. cerebralis was unrelated to surface charge, which suggests that relative to fish tissue, the surface of the spore is highly hydrophobic. The technique is useful for detection of small numbers of spores from populations of infected fish; however, the greatest value of the technique is the isolation of spores for biochemical and immunologic studies that are unmodified by the relatively harsh techniques usually used.Key words: Myxosoma cerebralis spores, Myxosoma spore isolation techniques, polymer aqueous two-phase systems

Porcine pancreatic lipase partition in potassium phosphate–polyethylene glycol aqueous two-phase systems

2007 ◽  
Vol 859 (2) ◽  
pp. 222-228 ◽  
Author(s):  
Georgina Bassani ◽  
Beatriz Farruggia ◽  
Bibiana Nerli ◽  
Diana Romanini ◽  
Guillermo Picó
Keyword(s):  
Polyethylene Glycol ◽  
Pancreatic Lipase ◽  
Potassium Phosphate ◽  
Porcine Pancreatic Lipase ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems
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