Including fluorescent nanoparticle probes within injectable gels for remote strain measurements and discrimination between compression and tension

Soft Matter ◽  
2021 ◽  
Author(s):  
Hannah R. Shanks ◽  
Shanglin Wu ◽  
Nam T. Nguyen ◽  
Dongdong Lu ◽  
Brian R. Saunders
Keyword(s):  
Fluorescent Probe ◽  
Remote Measurement ◽  
Strain Measurements ◽  
Nanoparticle Probes ◽  
Tension And Compression ◽  
Fluorescent Nanoparticle ◽  
Deformation Ratio ◽  
Injectable Gels ◽  
Probe Particles

Remote measurement of the deformation ratio and discrimination between tension and compression for injectable gels is demonstrated using photoluminescence and two types of fluorescent probe particles.

scholarly journals Enzyme-free amplified detection of cellular microRNA by light-harvesting fluorescent nanoparticle probes

2021 ◽  
Vol 179 ◽  
pp. 113084 ◽  
Author(s):  
Sylvie Egloff ◽  
Nina Melnychuk ◽  
Andreas Reisch ◽  
Sophie Martin ◽  
Andrey S. Klymchenko
Keyword(s):  
Light Harvesting ◽  
Nanoparticle Probes ◽  
Fluorescent Nanoparticle

scholarly journals Fluorescent Nanoparticle Probes for Cancer Imaging

2005 ◽  
Vol 4 (6) ◽  
pp. 593-602 ◽  
Author(s):  
Swadeshmukul Santra ◽  
Debamitra Dutta ◽  
Glenn A. Walter ◽  
Brij M. Moudgil
Keyword(s):  
Early Stage ◽  
Cancer Imaging ◽  
Cellular Level ◽  
Design Synthesis ◽  
Nanoparticle Probes ◽  
Non Invasive ◽  
Fluorescent Nanoparticle ◽  
Nanoparticle Design ◽  
Inexpensive Technique

Optical imaging technique has strong potential for sensitive cancer diagnosis, particularly at the early stage of cancer development. This is a sensitive, non-invasive, non-ionizing (clinically safe) and relatively inexpensive technique. Cancer imaging with optical technique however greatly relies upon the use of sensitive and stable optical probes. Unlike the traditional organic fluorescent probes, fluorescent nanoparticle probes such as dye-doped nanoparticles and quantum dots (Qdots) are bright and photostable. Fluorescent nanoparticle probes are shown to be very effective for sensitive cancer imaging with greater success in the cellular level. However, cancer imaging in an in vivo setup has been recently realized. There are several challenges in developing fluorescent nanoparticle probes for in vivo cancer imaging applications. In this review, we will discuss various aspects of nanoparticle design, synthesis, surface functionalization for bioconjugation and cancer cell targeting. A brief overview of in vivo cancer imaging with Qdots will also be presented.

scholarly journals Modular fluorescent nanoparticle DNA probes for detection of peptides and proteins

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cassandra M. Stawicki ◽  
Torri E. Rinker ◽  
Markus Burns ◽  
Sonal S. Tonapi ◽  
Rachel P. Galimidi ◽  
...  
Keyword(s):  
Fluorescent Labeling ◽  
Dna Aptamer ◽  
Covalent Attachment ◽  
Laboratory Equipment ◽  
Nanoparticle Probes ◽  
Biological Studies ◽  
Thermo Fisher Scientific ◽  
Fluorescent Nanoparticle ◽  
Aptamer Probe ◽  
Barriers To Use

AbstractFluorescently labeled antibody and aptamer probes are used in biological studies to characterize binding interactions, measure concentrations of analytes, and sort cells. Fluorescent nanoparticle labels offer an excellent alternative to standard fluorescent labeling strategies due to their enhanced brightness, stability and multivalency; however, challenges in functionalization and characterization have impeded their use. This work introduces a straightforward approach for preparation of fluorescent nanoparticle probes using commercially available reagents and common laboratory equipment. Fluorescent polystyrene nanoparticles, Thermo Fisher Scientific FluoSpheres, were used in these proof-of-principle studies. Particle passivation was achieved by covalent attachment of amine-PEG-azide to carboxylated particles, neutralizing the surface charge from − 43 to − 15 mV. A conjugation-annealing handle and DNA aptamer probe were attached to the azide-PEG nanoparticle surface either through reaction of pre-annealed handle and probe or through a stepwise reaction of the nanoparticles with the handle followed by aptamer annealing. Nanoparticles functionalized with DNA aptamers targeting histidine tags and VEGF protein had high affinity (EC50s ranging from 3 to 12 nM) and specificity, and were more stable than conventional labels. This protocol for preparation of nanoparticle probes relies solely on commercially available reagents and common equipment, breaking down the barriers to use nanoparticles in biological experiments.

Site-Specific Labeling of Surface Proteins on Living Cells Using Genetically Encoded Peptides that Bind Fluorescent Nanoparticle Probes

2009 ◽  
Vol 20 (8) ◽  
pp. 1482-1489 ◽  
Author(s):  
Mark A. Rocco ◽  
Jae-Young Kim ◽  
Andrew Burns ◽  
Jan Kostecki ◽  
Anne Doody ◽  
...  
Keyword(s):  
Living Cells ◽  
Surface Proteins ◽  
Site Specific ◽  
Nanoparticle Probes ◽  
Fluorescent Nanoparticle

scholarly journals Modular Fluorescent Nanoparticle DNA Probes for Detection of Peptides and Proteins

2021 ◽  
Author(s):  
Cassandra M. Stawicki ◽  
Torri E. Rinker ◽  
Markus Burns ◽  
Sonal S. Tonapi ◽  
Rachel P. Galimidi ◽  
...  
Keyword(s):  
Fluorescent Labeling ◽  
Dna Aptamer ◽  
Covalent Attachment ◽  
Polystyrene Nanoparticles ◽  
Laboratory Equipment ◽  
Nanoparticle Probes ◽  
Biological Studies ◽  
Fluorescent Nanoparticle ◽  
Aptamer Probe ◽  
Barriers To Use

Fluorescently labeled antibody and aptamer probes are used in biological studies to characterize binding interactions, measure concentrations of analytes, and sort cells. Fluorescent nanoparticle labels offer an excellent alternative to standard fluorescent labeling strategies due to their enhanced brightness, stability and multivalency; however, challenges in functionalization and characterization have impeded their use. This work introduces a straightforward approach for preparation of fluorescent nanoparticle probes using commercially available reagents and common laboratory equipment. Fluorescent polystyrene nanoparticles, Thermo Fisher FluoSpheres™, were used in proof-of-principle studies. Particle passivation was achieved by covalent attachment of amine-PEG-azide to carboxylated particles, neutralizing the surface charge from -47 to -17 mV. A conjugation-annealing handle and DNA aptamer probe was attached to the azide-PEG nanoparticle surface either through reaction of pre-annealed handle and probe or through a stepwise reaction of the nanoparticles with the handle followed by aptamer annealing. Nanoparticles functionalized with DNA aptamers targeting histidine tags and VEGF protein had high affinity (EC50s ranging from 2-7 nM) and specificity, and were more stable than conventional labels. This protocol for preparation of nanoparticle probes relies solely on commercially available reagents and common equipment, breaking down the barriers to use of nanoparticles in biological experiments.

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