Structural, mechanical, and biological characterization of hierarchical nanofibrous Fmoc-phenylalanine-valine hydrogels for 3D culture of differentiated and mesenchymal stem cells

Soft Matter ◽  
2021 ◽  
Vol 17 (1) ◽  
pp. 57-67
Author(s):  
Haniyeh Najafi ◽  
Ali Mohammad Tamaddon ◽  
Samira Abolmaali ◽  
Sedigheh Borandeh ◽  
Negar Azarpira
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Proliferative Activity ◽  
3D Culture ◽  
Shear Thinning ◽  
Biological Characterization ◽  
Cell Type

A shear-thinning Fmoc-phenylalanine-valine hydrogel exhibits cell type-dependent proliferative activity.

Isolation and Biological Characterization of Umbilical Cord Mesenchymal Stem Cells Derived from Goose Embryo

2018 ◽  
Vol 8 (6) ◽  
pp. 775-782
Author(s):  
Caiyun Ma ◽  
Kungfu Wang ◽  
Hongda Ji ◽  
Han Ren ◽  
Zhiyong Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Biological Characterization

Isolation and biological characterization of a novel type of pulmonary mesenchymal stem cells derived from Wuzhishan miniature pig embryo

2016 ◽  
Vol 40 (10) ◽  
pp. 1041-1049 ◽  
Author(s):  
Caiyun Ma ◽  
Yu Guo ◽  
Hao Liu ◽  
Kunfu Wang ◽  
Jinjuan Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Miniature Pig ◽  
Biological Characterization

Influence of Chondrocyte Zone on Co-Cultures With Mesenchymal Stem Cells in HA Hydrogels for Cartilage Tissue Engineering

2012 ◽  
Author(s):  
Minwook Kim ◽  
Jason A. Burdick ◽  
Robert L. Mauck
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Articular Chondrocytes ◽  
Cartilage Tissue Engineering ◽  
3D Culture ◽  
Cartilage Tissue ◽  
Cell Type

Mesenchymal stem cells (MSCs) are an attractive cell type for cartilage tissue engineering in that they can undergo chondrogenesis in a variety of 3D contexts [1]. Focused efforts in MSC-based cartilage tissue engineering have recently culminated in the formation of biologic materials possessing biochemical and functional mechanical properties that match that of the native tissue [2]. These approaches generally involve the continuous or intermittent application of pro-chondrogenic growth factors during in vitro culture. For example, in one recent study, we showed robust construct maturation in MSC-seeded hyaluronic acid (HA) hydrogels transiently exposed to high levels of TGF-β3 [3]. Despite the promise of this approach, MSCs are a multipotent cell type and retain a predilection towards hypertrophic phenotypic conversion (i.e., bone formation) when removed from a pro-chondrogenic environment (e.g., in vivo implantation). Indeed, even in a chondrogenic environment, many MSC-based cultures express pre-hypertrophic markers, including type X collagen, MMP13, and alkaline phosphatase [4]. To address this issue, recent studies have investigated co-culture of human articular chondrocytes and MSCs in both pellet and hydrogel environments. Chondrocytes appear to enhance the initial efficiency of MSC chondrogenic conversion, as well as limit hypertrophic changes in some instances (potentially via secretion of PTHrP and/or other factors) [5–7]. While these findings are intriguing, articular cartilage has a unique depth-dependent morphology including zonal differences in chondrocyte identity. Ng et al. showed that zonal chondrocytes seeded in a bi-layered agarose hydrogel construct can recreate depth-dependent cellular and mechanical heterogeneity, suggesting that these identities are retained with transfer to 3D culture systems [8]. Further, Cheng et al. showed that differences in matrix accumulation and hypertrophy in zonal chondrocytes was controlled by bone morphogenic protein [9]. To determine whether differences in zonal chondrocyte identity influences MSC fate decisions, we evaluated functional properties and phenotypic stability in photocrosslinked hyaluronic acid (HA) hydrogels using distinct, zonal chondrocyte cell fractions co-cultured with bone marrow derived MSCs.

scholarly journals Biological characterization of sheep kidney-derived mesenchymal stem cells

2016 ◽  
Vol 12 (6) ◽  
pp. 3963-3971 ◽  
Author(s):  
Meng Ji ◽  
Chunyu Bai ◽  
Lu Li ◽  
Ya'Nan Fan ◽  
Caiyun Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Biological Characterization

scholarly journals Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine

2014 ◽  
Vol 34 (2) ◽  
Author(s):  
Tingting Sun ◽  
Chao Yu ◽  
Yuhua Gao ◽  
Chenqiong Zhao ◽  
Jinlian Hua ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Types ◽  
Neural Cells ◽  
Biological Characterization ◽  
Multipotent Stem Cells ◽  
Reverse Transcription Pcr ◽  
Lineage Differentiation

The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT–PCR (reverse transcription–PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro. Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells.

Isolation and Biological Characterization of Heart-Derived Mesenchymal Stem Cells from Goose

2018 ◽  
Vol 8 (2) ◽  
pp. 182-189
Author(s):  
Yangnan Wu ◽  
Yanjie Zheng ◽  
Hongda Ji ◽  
Hebao Wen ◽  
Han Ren ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Biological Characterization

scholarly journals Biological characterization of mesenchymal stem cells from bovine umbilical cord

2014 ◽  
Vol 18 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Hui Xiong ◽  
Chunyu Bai ◽  
Shuang Wu ◽  
Yuhua Gao ◽  
Taofeng Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Biological Characterization

scholarly journals Biological characterization of metanephric mesenchymal stem cells from the Beijing duck

2015 ◽  
Vol 11 (2) ◽  
pp. 439-447
Author(s):  
JIA CHEN ◽  
YABIN PU ◽  
YUJIAO SUN ◽  
PING ZHANG ◽  
QIAN LI ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Biological Characterization

Biological characterization of long-term cultured human mesenchymal stem cells

2009 ◽  
Vol 32 (1) ◽  
pp. 117-126 ◽  
Author(s):  
Jiseon Kim ◽  
Jin Wook Kang ◽  
Jae Hyun Park ◽  
Youngju Choi ◽  
Kyung Suk Choi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Biological Characterization
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