A comparative study of antimicrobial activity of differently-synthesized chitosan nanoparticles against bovine mastitis pathogens

Soft Matter ◽  
2021 ◽  
Author(s):  
M. Soledad Orellano ◽  
Luciana P. Bohl ◽  
María L. Breser ◽  
Paula Isaac ◽  
R. Darío Falcone ◽  
...  

We synthesized Ch-NPs using different types of reverse micelles as nanoreactors. The aim was to study their antimicrobial activity against bovine mastitis pathogens, focusing on the survival mechanisms of pathogens and the interaction between Ch-NPs and host cells.

2019 ◽  
Vol 213 ◽  
pp. 1-9 ◽  
Author(s):  
María Soledad Orellano ◽  
Paula Isaac ◽  
Maria Laura Breser ◽  
Luciana Paola Bohl ◽  
Agustín Conesa ◽  
...  

1970 ◽  
Vol 5 (2) ◽  
pp. 117-121
Author(s):  
Othon José Ribeiro Pereira ◽  
José Marcos dos Reis

RESUMOObjetivo: Analisar, a partir da metodologia microbiológica, a ação bactericida dos diferentes tipos de mel, segundo sua florada de origem. Materiais e Métodos: foram adquiridas três amostras de cada tipo de mel, segundo sua florada de origem, sendo eles: Flores de Café, Cipó-Uva, Assa-Peixe, Limão, Laranjeira, Eucalipto e Silvestre. Os discos foram preparados sendo embebidos em mel, e mantidos em estufa para secagem. Estes foram então colocados em contato com placa de Petri, previamente preparadas com Ágar Nutriente, contendo Staphylococcus aureus, e colocados em estufa para secagem. Após 24 horas, observou-se se houve inibição da cepa bacteriana, formando halo ao redor do disco. Resultado: Notou-se, na leitura das 21 placas, que nao houve diferença significativa nos halos de inibição formados pelos diferentes tipos de mel testados. Conclusão: Pelo estudo realizado, pode-se concluir que não há diferença na ação bactericida, sobre cepa de Staphylococcus aureus, entre os diferentes tipos de mel segundo as floradas de origem testadas.Palavras chave: Anti-Infecciosos, Mel, Staphylococcus aureus, Faringe. ABSTRACTObjective: To analyze, according to microbiological methodology, the bactericidal action of different ypes on honey, according to the flowering origin. Materials and Methods: three samples of each types of honey were acquired, according to the flowering of origin, namely: Coffee Flowers, Vine-Grape, Assa-Peixe, Lemon, Orange, Eucalyptus and Sylvan. The disks were prepared by being soaked in honey, and were kept in a kilnfor drying. These were then placed in contact with Petri dish, previously prepared with Nutrient Agar, containing Staphylococcus aureus, and placed in kiln for drying. After 24 hours, it was observed if there was inhibition of bacterial strain, by forming a halo around the disk. Results: It was noted, in the reading of the 21 plates, no significant difference in the inhibition halos formed by different types of honey tested. Conclusion:By the study, it can be concluded that there is no difference in bactericidal action on strain of Stapylococcus aureus, between the different types of honey according to the flowering origin tested.Key words: Anti-Infective Agents, Honey, Staphylococcus aureus, Phayngitis.    


Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
M Niculae ◽  
CD Sandru ◽  
E Pall ◽  
S Popescu ◽  
L Stan ◽  
...  

1977 ◽  
Vol 79 (1) ◽  
pp. 133-140 ◽  
Author(s):  
H. M. Meddick

SUMMARYThe ability of six different types of contamination control mats currently in use at the entrances to theatre suites and other clean areas to remove bacteria-carrying particles from theatre trolley wheels was compared. Marked differences in the effectiveness of this property were obtained; and all mats showed some disadvantages. Modification of one of the mats has resulted in improved efficiency under working conditions.


2021 ◽  
Vol 88 (1) ◽  
pp. 80-88
Author(s):  
Remo Stürmlin ◽  
Josef J. Gross ◽  
Olga Wellnitz ◽  
Lea A. Wagner ◽  
Camille Monney ◽  
...  

AbstractThe aim of the present study was to investigate the effects of milk composition changes on the in vitro growth of bovine mastitis pathogens. Nutritional requirements of three major bovine mastitis pathogens Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Streptococcus uberis (S. uberis) were investigated in vitro. We used ultra-high temperature (UHT) treated milk with different contents of fat, protein, and carbohydrates to test the influence of the availability of various milk constituents on pathogen growth characteristics. Additionally, the bacterial growth was investigated under experimentally modified nutrient availability by dilution and subsequent supplementation with individual nutrients (carbohydrates, different nitrogen sources, minerals, and different types of B vitamins) either to milk or to a conventional medium (thioglycolate broth, TB). Varying contents of fat, protein or lactose did not affect bacterial growth with the exception of growth of S. uberis being promoted in protein-enriched milk. The addition of nutrients to diluted whole milk and TB partly revealed different effects, indicating that there are media-specific growth limiting factors after dilution. Supplementation of minerals to diluted milk did not affect growth rates of all studied bacteria. Bacterial growth in diluted whole milk was decreased by the addition of high concentrations of amino acids in S. aureus, and by urea and additional B vitamins in E. coli and S. aureus. The growth rate of S. uberis was increased by the addition of B vitamins to diluted whole milk. The present results demonstrate that growth-limiting nutrients differ among pathogen types. Because reduced bacterial growth was only shown in diluted milk or TB, it is unlikely that alterations in nutrient availability occurring as a consequence of physiological changes of milk composition in the cow's udder would directly affect the susceptibility or course of bovine mastitis.


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