scholarly journals Characterization and minimization of band broadening in DNA electrohydrodynamic migration for enhanced size separation

Soft Matter ◽  
2020 ◽  
Vol 16 (24) ◽  
pp. 5640-5649
Author(s):  
Jeffrey Teillet ◽  
Quentin Martinez ◽  
Inga Tijunelyte ◽  
Bayan Chami ◽  
Aurélien Bancaud
Keyword(s):  
Microfluidic Chip ◽  
Dna Separation ◽  
Band Broadening ◽  
Size Separation

We report a detailed study of band broadening in a microfluidic chip for DNA separation by means of electrohydrodynamic viscoelatic migration.

scholarly journals Modeling of DNA transport in viscoelastic electro-hydrodynamic flows for enhanced size separation

Soft Matter ◽  
2018 ◽  
Vol 14 (24) ◽  
pp. 5069-5079 ◽  
Author(s):  
B. Chami ◽  
M. Socol ◽  
M. Manghi ◽  
A. Bancaud
Keyword(s):  
Body Fluids ◽  
Dna Separation ◽  
Microfluidic Systems ◽  
Dna Transport ◽  
Size Separation ◽  
Disease Identification

DNA separation and analysis have advanced over recent years, benefiting from microfluidic systems that reduce sample volumes and analysis costs, essential for sequencing and disease identification in body fluids.

scholarly journals DNA separation and enrichment using electro-hydrodynamic bidirectional flows in viscoelastic liquids

Lab on a Chip ◽  
2016 ◽  
Vol 16 (7) ◽  
pp. 1243-1253 ◽  
Author(s):  
Hubert Ranchon ◽  
Rémi Malbec ◽  
Vincent Picot ◽  
Audrey Boutonnet ◽  
Pattamon Terrapanich ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
Dna Separation ◽  
Size Separation ◽  
Viscoelastic Liquids ◽  
Dna Size

DNA size separation followed by purification and enrichment constitute essential operations for genetic engineering.

Toward a More General Solution to the Band-Broadening Problem in Size Separation of Polymers

2007 ◽  
Vol 40 (9) ◽  
pp. 3477-3487 ◽  
Author(s):  
Dominik Konkolewicz ◽  
James W. Taylor ◽  
Patrice Castignolles ◽  
Angus Gray-Weale ◽  
Robert G. Gilbert
Keyword(s):  
General Solution ◽  
Band Broadening ◽  
Size Separation ◽  
Separation Of Polymers

Drop driving on digital microfluidic chip

2020 ◽  
Vol 28 (11) ◽  
pp. 2488-2496
Author(s):  
Hong WANG ◽  
◽  
Jie ZHENG ◽  
Yan-peng YAN ◽  
Song WANG ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Digital Microfluidic

An Enrichment Method for Bio-samples Based on Circular Injection on a Microfluidic Chip

2013 ◽  
Vol 40 (11) ◽  
pp. 1668-1673
Author(s):  
Min DU ◽  
Xiong-Ying YE ◽  
Jin-Yang FENG ◽  
Zeng-Shuai MA ◽  
Zhao-Ying ZHOU
Keyword(s):  
Microfluidic Chip ◽  
Enrichment Method

An enzyme reactor based on aptamer modified microfluidic chip for protein analysis

2013 ◽  
Vol 30 (11) ◽  
pp. 1127-1132 ◽  
Author(s):  
Peng XIAO ◽  
Dalei LI ◽  
Yan MAN ◽  
Lina GENG ◽  
Xuefei LU ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Protein Analysis ◽  
Enzyme Reactor

scholarly journals Microfluidic Chip for Detection of Fungal Infections

ACS Omega ◽  
2019 ◽  
Vol 4 (4) ◽  
pp. 7474-7481 ◽  
Author(s):  
Waseem Asghar ◽  
Mazhar Sher ◽  
Nida S. Khan ◽  
Jatin M. Vyas ◽  
Utkan Demirci
Keyword(s):  
Microfluidic Chip ◽  
Fungal Infections

scholarly journals An LED-Driven AuNPs-PDMS Microfluidic Chip and Integrated Device for the Detection of Digital Loop-Mediated Isothermal DNA Amplification

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 177 ◽  
Author(s):  
Zengming Zhang ◽  
Shuhao Zhao ◽  
Fei Hu ◽  
Guangpu Yang ◽  
Juan Li ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Microfluidic Chip ◽  
Cross Flow ◽  
Flow Channel ◽  
Isothermal Amplification ◽  
Fluorescent Detection ◽  
Lamp Method ◽  
Biological Studies ◽  
Micro Cavity ◽  
Integrated Device

The sensitive quantification of low-abundance nucleic acids holds importance for a range of clinical applications and biological studies. In this study, we describe a facile microfluidic chip for absolute DNA quantifications based on the digital loop-mediated isothermal amplification (digital LAMP) method. This microfluidic chip integrates a cross-flow channel for droplet generation with a micro-cavity for droplet tiling. DNA templates in the LAMP reagent were divided into ~20,000 water-in-oil droplets at the cross-flow channel. The droplets were then tiled in the micro-cavity for isothermal amplification and fluorescent detection. Different from the existing polydimethylsiloxane (PDMS) microfluidic chips, this study incorporates gold nanoparticles (AuNPs) into PDMS substrate through silica coating and dodecanol modification. The digital LAMP chip prepared by AuNPs-PDMS combines the benefits of the microstructure manufacturing performance of PDMS with the light-to-heat conversion advantages of AuNPs. Upon illumination with a near infrared (NIR) LED, the droplets were stably and efficiently heated by the AuNPs in PDMS. We further introduce an integrated device with a NIR heating unit and a fluorescent detection unit. The system could detect HBV (hepatitis B virus)-DNA at a concentration of 1 × 101 to 1 × 104 copies/μL. The LED-driven digital LAMP chip and the integrated device; therefore, demonstrate high accuracy and excellent performance for the absolute quantification of low-abundance nucleic acids, showing the advantages of integration, miniaturization, cost, and power consumption.

Passive micromixer integration with a microfluidic chip for calcium assay based on the arsenazo III method

2011 ◽  
Vol 5 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Yuwadee Boonyasit ◽  
Thitima Maturos ◽  
Assawapong Sappat ◽  
Apichai Jomphoak ◽  
Adisorn Tuantranont ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Arsenazo Iii ◽  
Passive Micromixer
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