scholarly journals Lipidomic atlas of mammalian cell membranes reveals hierarchical variation induced by culture conditions, subcellular membranes, and cell lineages

Soft Matter ◽  
2021 ◽  
Author(s):  
Jessica L. Symons ◽  
Kwang-Jin Cho ◽  
Jeffrey T. Chang ◽  
Guangwei Du ◽  
M. Neal Waxham ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Lipid Membranes ◽  
Cell Membranes ◽  
Culture Conditions ◽  
Cell Lineages ◽  
Cellular Organelles

Lipid membranes are ubiquitous biological organizers, required for structural and functional compartmentalization of the cell and sub-cellular organelles.

scholarly journals Effects of lipid heterogeneity on model human brain lipid membranes

Soft Matter ◽  
2021 ◽  
Vol 17 (1) ◽  
pp. 126-135
Author(s):  
Sze May Yee ◽  
Richard J. Gillams ◽  
Sylvia E. McLain ◽  
Christian D. Lorenz
Keyword(s):  
Human Brain ◽  
Lipid Membranes ◽  
Cell Membranes ◽  
Brain Lipid ◽  
Lipid Distribution

Cell membranes naturally contain a heterogeneous lipid distribution.

Elucidation of molecular interactions between lipid membranes and ionic liquids using model cell membranes

Soft Matter ◽  
2012 ◽  
Vol 8 (20) ◽  
pp. 5501 ◽  
Author(s):  
Seunghwan Jeong ◽  
Sung Ho Ha ◽  
Sang-Hyun Han ◽  
Min-Cheol Lim ◽  
Sun Min Kim ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Molecular Interactions ◽  
Lipid Membranes ◽  
Cell Membranes ◽  
Model Cell

scholarly journals Identification of the glucose transporter in mammalian cell membranes with a125I-forskolin photoaffinity label

1988 ◽  
Vol 255 (3) ◽  
pp. 983-990 ◽  
Author(s):  
B E Wadzinski ◽  
M F Shanahan ◽  
R B Clark ◽  
A E Ruoho
Keyword(s):  
Smooth Muscle ◽  
Molecular Mass ◽  
Mammalian Cell ◽  
Cell Membranes ◽  
Plasma Membranes ◽  
Glucose Transporter ◽  
Apparent Molecular Mass ◽  
Synaptic Membranes ◽  
Placental Membranes ◽  
Adipocyte Plasma Membranes

The glucose transporter has been identified in a variety of mammalian cell membranes using a photoactivatable carrier-free radioiodinated derivative of forskolin, 3-[125I]iodo-4-azidophenethylamido-7-O-succinyldeacetylforskoli n ([125I]IAPS-forskolin) at 1-3 nM. The membranes that were photolabelled with [125I]IAPS-forskolin were human placental membranes, rat cortical and cerebellar synaptic membranes, rat cardiac sarcolemmal membranes, rat adipocyte plasma membranes, smooth-muscle membranes, and S49 wild-type (WT) lymphoma-cell membranes. The glucose transporter in plasma membranes prepared from the insulin-responsive rat cardiac sarcolemmal cells, rat adipocytes and smooth-muscle cells were determined to be approx. 45 kDa by SDS/polyacrylamide-gel electrophoresis (PAGE). Photolysis of human placental membranes, rat cortical and cerebellar synaptic membranes, and WT lymphoma membranes with [125I]-IAPS-forskolin, followed by SDS/PAGE, indicated specific derivatization of a broad band (43-55 kDa) in placental membranes and a narrower band (approx. 45 kDa) in synaptic membranes and WT lymphoma membranes. Digestion of the [125I]IAPS-forskolin-labelled placental and WT lymphoma membranes with endo-beta-galactosidase showed a reduction in the apparent molecular mass of the radiolabelled band to approx. 40 kDa. The membranes that were photolabelled with [125I]IAPS-forskolin and trypsin-treated produced a radiolabelled proteolytic fragment with an apparent molecular mass of 18 kDa. [125I]IAPS-forskolin is a highly effective probe for identifying low levels of glucose transporters in mammalian tissues.

Singlet oxygen effects on lipid membranes: implications for the mechanism of action of broad-spectrum viral fusion inhibitors

2014 ◽  
Vol 459 (1) ◽  
pp. 161-170 ◽  
Author(s):  
Axel Hollmann ◽  
Miguel A. R. B. Castanho ◽  
Benhur Lee ◽  
Nuno C. Santos
Keyword(s):  
Singlet Oxygen ◽  
Mechanism Of Action ◽  
Broad Spectrum ◽  
Lipid Membranes ◽  
Cell Membranes ◽  
Viral Fusion ◽  
Fusion Inhibitors ◽  
Oxygen Effects

By studying the interaction of LJ001 and JL103 with lipid membranes, we demonstrate that singlet oxygen produced by both compounds induces changes on lipid properties, preventing the fusion between viral and cell membranes.

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