scholarly journals The structure of human dermatan sulfate epimerase 1 emphasizes the importance of C5-epimerization of glucuronic acid in higher organisms

2021 ◽  
Author(s):  
Mahmudul Hasan ◽  
Hamed Khakzad ◽  
Lotta Happonen ◽  
Anders Sundin ◽  
Johan Unge ◽  
...  
Keyword(s):  
Glucuronic Acid ◽  
Dermatan Sulfate ◽  
Structural Studies ◽  
Bacterial Proteins ◽  
Catalytic Isomerization ◽  
Isomerization Mechanism

Structural studies of human DS-epi1 suggests a new catalytic isomerization mechanism and reveals remarkable similarities to bacterial proteins.

THE POLYSACCHARIDES OF CRYPTOCOCCUS LAURENTII (NRRL Y-1401): PART I

1960 ◽  
Vol 38 (9) ◽  
pp. 1617-1624 ◽  
Author(s):  
M. J. Abercrombie ◽  
J. K. N. Jones ◽  
M. V. Lock ◽  
M. B. Perry ◽  
R. J. Stoodley
Keyword(s):  
Glucuronic Acid ◽  
Extracellular Polysaccharides ◽  
Cryptococcus Laurentii ◽  
Structural Studies ◽  
Acidic Polysaccharide ◽  
End Groups

The extracellular polysaccharides produced by Cryptococcuslaurentii have been isolated and shown to consist of (A) an acidic polysaccharide containing D-mannose, D-xylose, and D-glucuronic acid; (B) a neutral polysaccharide containing D-glucose only.Preliminary structural studies on the acidic material suggest that it consists of a mannose-containing backbone with xylose and glucuronic acid as end groups, while the glucan contains 1 → 3, 1 → 4, 1 → 2, and (or) 1 → 6 linked residues.

scholarly journals Structural Studies on the Hexasaccharide Alditols Isolated from the Carbohydrate-Protein Linkage Region of Dermatan Sulfate Proteoglycans of Bovine Aorta

1995 ◽  
Vol 270 (13) ◽  
pp. 7204-7212 ◽  
Author(s):  
Kazuyuki Sugahara ◽  
Yumi Ohkita ◽  
Yuniko Shibata ◽  
Keiichi Yoshida ◽  
Akemi Ikegami
Keyword(s):  
Dermatan Sulfate ◽  
Structural Studies ◽  
Linkage Region

scholarly journals Structural studies on colanic acid, the common exopolysaccharide found in the Enterobacteriaceae, by partial acid hydrolysis. Oligosaccharides from colanic acid

1969 ◽  
Vol 115 (5) ◽  
pp. 935-945 ◽  
Author(s):  
I. W. Sutherland
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Pyruvic Acid ◽  
Glucuronic Acid ◽  
Periodate Oxidation ◽  
Structural Studies ◽  
Acid Biosynthesis ◽  
Partial Acid Hydrolysis ◽  
Colanic Acid ◽  
The Common

The exopolysaccharide slime colanic acid has been isolated from representative strains of Escherichia coli, Salmonella typhimurium and Aerobacter cloacae. Analysis showed that each polymer contained glucose, galactose, fucose and glucuronic acid, together with acetate and pyruvate. The molar proportions of these components were 1:1·8:1·9:1:1:1 approximately. On the basis of periodate oxidation of the natural and deacetylated polysaccharide, glucose is proposed as the site of the acetyl groups. The pyruvate is attached to galactose. Three neutral oligosaccharides and ten electrophoretically mobile oligosaccharides were isolated and partially characterized. Four of the fragments were esters of pyruvic acid. Most oligosaccharides were isolated from all three polysaccharide preparations. Three further oligosaccharides were isolated from carboxyl-reduced colanic acid and sodium borotritide was used to label the glucose derived from glucuronic acid in these fragments. One trisaccharide was obtained from periodate-oxidized polysaccharide. On the basis of these oligosaccharides a repeating hexasaccharide unit of the following structure is proposed: [Formula: see text] The significance of this structure in colanic acid biosynthesis is discussed.

Fractional isolation and structural characterisation of hemicellulosic polymers from delignified and ultrasonic irradiated sugarcane bagasse

e-Polymers ◽  
2006 ◽  
Vol 6 (1) ◽  
Author(s):  
Jui-Li Ren ◽  
Zeng-Chao Geng ◽  
Chan-Fu Liu ◽  
Feng Xu ◽  
Jin-Xia Sun ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Glucuronic Acid ◽  
Structural Studies ◽  
Sugar Composition ◽  
Total Sugars ◽  
Structural Characterisation ◽  
Molecular Weights ◽  
Second Stage ◽  
Total Dissolution ◽  
C Nmr

AbstractHemicellulose-type polysaccharides were isolated from the delignified and ultrasonic irradiated sugarcane bagasse by a sequential two-step alkaline extraction. It was found that the successive extractions with 15% and 18% KOH for 2 h, 15% and 18% NaOH for 2 h, 8% and 10% KOH for 15 h, and with 8% and 10% NaOH for 15 h resulted in a total dissolution of 89.6%, 92.8%, 94.9%, and 97.3% of the original hemicelluloses, respectively. Sugar analysis revealed that xylose was the predominant sugar composition of all the hemicelluloses, comprising 57.4- 68.6% of the total sugars. Arabinose (12.3-18.4%) and glucose (10.8- 14.6%) appeared as the second and third major sugar constituents. Galactose (3.9-8.7%), uronic acids, mainly 4-O-methyl-glucuronic acid (2.7-5.8%), rhamnose (1.2-2.6%), and mannose (0.2-1.3%) were observed as minor constituents. The structural studies by 13C-NMR spectroscopy showed that L-arabino (4-O-methyl-D-glucurono) xylans were the major constituents of the hemicellulosic polymers. Furthermore, the current results also showed that the four alkali-soluble hemicellulosic fractions, isolated during the first step treatment with relatively lower concentrations of alkalis, were more branched and acidic, and had larger molecular weights (Mw, 23100-34500), but lower thermal stability than the other four alkali-soluble hemicellulosic preparations (Mw, 21700-28700), extracted during the second stage treatment with relatively higher concentrations of alkalis.

scholarly journals Dermatan Sulfate Epimerase 1-Deficient Mice Have Reduced Content and Changed Distribution of Iduronic Acids in Dermatan Sulfate and an Altered Collagen Structure in Skin

2009 ◽  
Vol 29 (20) ◽  
pp. 5517-5528 ◽  
Author(s):  
Marco Maccarana ◽  
Sebastian Kalamajski ◽  
Mads Kongsgaard ◽  
S. Peter Magnusson ◽  
Åke Oldberg ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Tensile Strength ◽  
Collagen Fibril ◽  
Glucuronic Acid ◽  
Dermatan Sulfate ◽  
Collagen Structure ◽  
Wild Type ◽  
Iduronic Acid ◽  
Skin Collagen ◽  
Deficient Mice

ABSTRACT Dermatan sulfate epimerase 1 (DS-epi1) and DS-epi2 convert glucuronic acid to iduronic acid in chondroitin/dermatan sulfate biosynthesis. Here we report on the generation of DS-epi1-null mice and the resulting alterations in the chondroitin/dermatan polysaccharide chains. The numbers of long blocks of adjacent iduronic acids are greatly decreased in skin decorin and biglycan chondroitin/dermatan sulfate, along with a parallel decrease in iduronic-2-O-sulfated-galactosamine-4-O-sulfated structures. Both iduronic acid blocks and iduronic acids surrounded by glucuronic acids are also decreased in versican-derived chains. DS-epi1-deficient mice are smaller than their wild-type littermates but otherwise have no gross macroscopic alterations. The lack of DS-epi1 affects the chondroitin/dermatan sulfate in many proteoglycans, and the consequences for skin collagen structure were initially analyzed. We found that the skin collagen architecture was altered, and electron microscopy showed that the DS-epi1-null fibrils have a larger diameter than the wild-type fibrils. The altered chondroitin/dermatan sulfate chains carried by decorin in skin are likely to affect collagen fibril formation and reduce the tensile strength of DS-epi1-null skin.

Structural studies of human placental dermatan sulfate during development using optical mixing spectroscopy

Biopolymers ◽  
1974 ◽  
Vol 13 (10) ◽  
pp. 2133-2146 ◽  
Author(s):  
Alexander M. Jamieson ◽  
Ting-Yang Lee ◽  
Irwin A. Schafer
Keyword(s):  
Dermatan Sulfate ◽  
Structural Studies ◽  
Optical Mixing ◽  
Optical Mixing Spectroscopy
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