scholarly journals Phenazines as model low-midpoint potential electron shuttles for photosynthetic bioelectrochemical systems

2021 ◽  
Author(s):  
Eleanor R. Clifford ◽  
Robert W. Bradley ◽  
Laura T. Wey ◽  
Joshua M. Lawrence ◽  
Xiaolong Chen ◽  
...  
Keyword(s):  
Bioelectrochemical Systems ◽  
Midpoint Potential ◽  
Electron Shuttles

Phenazines were explored as novel low-midpoint potential molecules for wiring cyanobacteria to electrodes.

scholarly journals Laccases: Versatile Biocatalysts for the Synthesis of Heterocyclic Cores

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3719
Author(s):  
Ana Catarina Sousa ◽  
Lígia O. Martins ◽  
M. Paula Robalo
Keyword(s):  
Membered Ring ◽  
Low Molecular Weight ◽  
Redox Potentials ◽  
Redox Mediators ◽  
Multicopper Oxidases ◽  
Phenothiazine Derivatives ◽  
Ph Values ◽  
Electron Shuttles ◽  
Specific Oxidation ◽  
Optimal Ph

Laccases are multicopper oxidases that have shown a great potential in various biotechnological and green chemistry processes mainly due to their high relative non-specific oxidation of phenols, arylamines and some inorganic metals, and their high redox potentials that can span from 500 to 800 mV vs. SHE. Other advantages of laccases include the use of readily available oxygen as a second substrate, the formation of water as a side-product and no requirement for cofactors. Importantly, addition of low-molecular-weight redox mediators that act as electron shuttles, promoting the oxidation of complex bulky substrates and/or of higher redox potential than the enzymes themselves, can further expand their substrate scope, in the so-called laccase-mediated systems (LMS). Laccase bioprocesses can be designed for efficiency at both acidic and basic conditions since it is known that fungal and bacterial laccases exhibit distinct optimal pH values for the similar phenolic and aromatic amines. This review covers studies on the synthesis of five- and six-membered ring heterocyclic cores, such as benzimidazoles, benzofurans, benzothiazoles, quinazoline and quinazolinone, phenazine, phenoxazine, phenoxazinone and phenothiazine derivatives. The enzymes used and the reaction protocols are briefly outlined, and the mechanistic pathways described.

scholarly journals How to balance the voltage in serially stacked bioelectrochemical systems

2021 ◽  
Vol 491 ◽  
pp. 229576
Author(s):  
Daniele Molognoni ◽  
Pau Bosch-Jimenez ◽  
Jordi Suarez ◽  
Monica Della Pirriera ◽  
Eduard Borràs
Keyword(s):  
Bioelectrochemical Systems

scholarly journals Status Update on Bioelectrochemical Systems: Prospects for Carbon Electrode Design and Scale-Up

Catalysts ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 278
Author(s):  
Katharina Herkendell
Keyword(s):  
Gradual Increase ◽  
Scale Up ◽  
Value Added ◽  
Biofuel Cells ◽  
Carbon Electrode ◽  
Electrode Design ◽  
Bioelectrochemical Systems ◽  
Electrode Assemblies ◽  
The Stability ◽  
Scientific Attention

Bioelectrochemical systems (BES) employ enzymes, subcellular structures or whole electroactive microorganisms as biocatalysts for energy conversion purposes, such as the electrosynthesis of value-added chemicals and power generation in biofuel cells. From a bioelectrode engineering viewpoint, customizable nanostructured carbonaceous matrices have recently received considerable scientific attention as promising electrode supports due to their unique properties attractive to bioelectronics devices. This review demonstrates the latest advances in the application of nano- and micro-structured carbon electrode assemblies in BES. Specifically, in view of the gradual increase in the commercial applicability of these systems, we aim to address the stability and scalability of different BES designs and to highlight their potential roles in a circular bioeconomy.

scholarly journals Spectroscopic evidence for direct flavin-flavin contact in a bifurcating electron transfer flavoprotein

2020 ◽  
Vol 295 (36) ◽  
pp. 12618-12634
Author(s):  
H. Diessel Duan ◽  
Nishya Mohamed-Raseek ◽  
Anne-Frances Miller
Keyword(s):  
Electron Transfer ◽  
Density Functional ◽  
Protein Denaturation ◽  
Rhodopseudomonas Palustris ◽  
Density Functional Theory Calculations ◽  
Site Directed Mutagenesis ◽  
Functional Theory ◽  
Electron Transfer Flavoprotein ◽  
Midpoint Potential ◽  
Ct Complex

A remarkable charge transfer (CT) band is described in the bifurcating electron transfer flavoprotein (Bf-ETF) from Rhodopseudomonas palustris (RpaETF). RpaETF contains two FADs that play contrasting roles in electron bifurcation. The Bf-FAD accepts electrons pairwise from NADH, directs one to a lower-reduction midpoint potential (E°) carrier, and the other to the higher-E° electron transfer FAD (ET-FAD). Previous work noted that a CT band at 726 nm formed when ET-FAD was reduced and Bf-FAD was oxidized, suggesting that both flavins participate. However, existing crystal structures place them too far apart to interact directly. We present biochemical experiments addressing this conundrum and elucidating the nature of this CT species. We observed that RpaETF missing either FAD lacked the 726 nm band. Site-directed mutagenesis near either FAD produced altered yields of the CT species, supporting involvement of both flavins. The residue substitutions did not alter the absorption maximum of the signal, ruling out contributions from residue orbitals. Instead, we propose that the residue identities modulate the population of a protein conformation that brings the ET-flavin and Bf-flavin into direct contact, explaining the 726 nm band based on a CT complex of reduced ET-FAD and oxidized Bf-FAD. This is corroborated by persistence of the 726 nm species during gentle protein denaturation and simple density functional theory calculations of flavin dimers. Although such a CT complex has been demonstrated for free flavins, this is the first observation of such, to our knowledge, in an enzyme. Thus, Bf-ETFs may optimize electron transfer efficiency by enabling direct flavin-flavin contact.

scholarly journals Strain- and Substrate-Dependent Redox Mediator and Electricity Production by Pseudomonas aeruginosa

2016 ◽  
Vol 82 (16) ◽  
pp. 5026-5038 ◽  
Author(s):  
Erick M. Bosire ◽  
Lars M. Blank ◽  
Miriam A. Rosenbaum
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Electron Transfer ◽  
Fuel Cells ◽  
Microbial Fuel Cells ◽  
Pure Culture ◽  
Bioelectrochemical Systems ◽  
Content Type ◽  
Mediated Electron Transfer ◽  
Phenazine Production ◽  
Model Strain

ABSTRACTPseudomonas aeruginosais an important, thriving member of microbial communities of microbial bioelectrochemical systems (BES) through the production of versatile phenazine redox mediators. Pure culture experiments with a model strain revealed synergistic interactions ofP. aeruginosawith fermenting microorganisms whereby the synergism was mediated through the shared fermentation product 2,3-butanediol. Our work here shows that the behavior and efficiency ofP. aeruginosain mediated current production is strongly dependent on the strain ofP. aeruginosa. We compared levels of phenazine production by the previously investigated model strainP. aeruginosaPA14, the alternative model strainP. aeruginosaPAO1, and the BES isolatePseudomonassp. strain KRP1 with glucose and the fermentation products 2,3-butanediol and ethanol as carbon substrates. We found significant differences in substrate-dependent phenazine production and resulting anodic current generation for the three strains, with the BES isolate KRP1 being overall the best current producer and showing the highest electrochemical activity with glucose as a substrate (19 μA cm−2with ∼150 μg ml−1phenazine carboxylic acid as a redox mediator). Surprisingly,P. aeruginosaPAO1 showed very low phenazine production and electrochemical activity under all tested conditions.IMPORTANCEMicrobial fuel cells and other microbial bioelectrochemical systems hold great promise for environmental technologies such as wastewater treatment and bioremediation. While there is much emphasis on the development of materials and devices to realize such systems, the investigation and a deeper understanding of the underlying microbiology and ecology are lagging behind. Physiological investigations focus on microorganisms exhibiting direct electron transfer in pure culture systems. Meanwhile, mediated electron transfer with natural redox compounds produced by, for example,Pseudomonas aeruginosamight enable an entire microbial community to access a solid electrode as an alternative electron acceptor. To better understand the ecological relationships between mediator producers and mediator utilizers, we here present a comparison of the phenazine-dependent electroactivities of threePseudomonasstrains. This work forms the foundation for more complex coculture investigations of mediated electron transfer in microbial fuel cells.

Electrochemical communication between microbial cells and electrodes via osmium redox systems

2012 ◽  
Vol 40 (6) ◽  
pp. 1330-1335 ◽  
Author(s):  
Kamrul Hasan ◽  
Sunil A. Patil ◽  
Dónal Leech ◽  
Cecilia Hägerhäll ◽  
Lo Gorton
Keyword(s):  
Electron Transfer ◽  
Biofuel Cells ◽  
Major Group ◽  
Electrochemical Biosensors ◽  
Bioelectrochemical Systems ◽  
Bacterial Cells ◽  
Redox Systems ◽  
Microbial Cells ◽  
Fundamental Part ◽  
Micro Organisms

Electrochemical communication between micro-organisms and electrodes is the integral and fundamental part of BESs (bioelectrochemical systems). The immobilization of bacterial cells on the electrode and ensuring efficient electron transfer to the electrode via a mediator are decisive features of mediated electrochemical biosensors. Notably, mediator-based systems are essential to extract electrons from the non-exoelectrogens, a major group of microbes in Nature. The advantage of using polymeric mediators over diffusible mediators led to the design of osmium redox polymers. Their successful use in enzyme-based biosensors and BFCs (biofuel cells) paved the way for exploring their use in microbial BESs. The present mini-review focuses on osmium-bound redox systems used to date in microbial BESs and their role in shuttling electrons from viable microbial cells to electrodes.

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