scholarly journals A molecular approach to rationally constructing specific fluorogenic substrates for the detection of acetylcholinesterase activity in live cells, mice brains and tissues

2020 ◽  
Vol 11 (41) ◽  
pp. 11285-11292 ◽  
Author(s):  
Xiaofeng Wu ◽  
Jong Min An ◽  
Jizhen Shang ◽  
Eugene Huh ◽  
Sujie Qi ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Ache Activity ◽  
Normal Mouse ◽  
High Selectivity ◽  
Acetylcholinesterase Activity ◽  
Live Cells ◽  
Molecular Approach ◽  
Fluorogenic Substrates ◽  
New Approach ◽  
Normal Mouse Brain

In this work, a new approach was developed for designing the representative P10 with high selectivity and sensitivity for imaging AChE activity in the cells and normal mouse brain.

scholarly journals Myelin basic protein is affected by reduced synthesis of myelin proteolipid protein in the jimpy mouse

1990 ◽  
Vol 268 (1) ◽  
pp. 105-110 ◽  
Author(s):  
A M Fannon ◽  
M A Moscarello
Keyword(s):  
Glutamic Acid ◽  
Mouse Brain ◽  
Gel Electrophoresis ◽  
Normal Mouse ◽  
Proteolipid Protein ◽  
Jimpy Mouse ◽  
Jimpy Mice ◽  
Size Heterogeneity ◽  
Complement Component C8 ◽  
Normal Mouse Brain

Myelin basic proteins (MBPs) from 6-day-old, 10-day-old, 20-day-old and adult normal mouse brain were compared with those from 20-day-old jimpy (dysmyelinating mutant) mouse brain to determine the effect of reduced levels of proteolipid protein (PLP) on MBPs. Alkaline-urea-gel electrophoresis showed that 6-day-old and 10-day-old normal and jimpy MBPs lacked charge microheterogeneity, since C8 (the least cationic of the components; not be confused with complement component C8) was the only charge isomer present. In contrast, MBPs from 20-day-old and adult normal mouse brain displayed extensive charge microheterogeneity, having at least eight components. A 32 kDa MBP was the major isoform observed on immunoblots of acid-soluble protein from 6-day-old and 10-day-old normal and 20-day-old jimpy mouse brain. There were eight bands present in 20-day-old and adult normal mouse brain. Purified human MBP charge heteromers C1, C2, C3 and C4 reacted strongly with rat 14 kDa MBP antiserum, whereas the reaction with human C8 was weak. This suggested that MBPs from early-myelinating and jimpy mice did not react to MBP antisera because C8 was the major charge isomer in these animals. Purification of MBPs from normal and jimpy brain by alkaline-gel electrophoresis showed that both normal and jimpy MBPs have size heterogeneity when subjected to SDS/PAGE. However, the size isoforms in normal mouse brain (32, 21, 18.5, 17 and 14 kDa) differed from those in jimpy brain (32, 21, 20, 17, 15 and 14 kDa) in both size and relative amounts. Amino acid analyses of MBPs from jimpy brain showed an increase in glutamic acid, alanine and ornithine, and a decrease in histidine, arginine and proline. The changes in glutamic acid, ornithine and arginine are characteristic of the differences observed in human C8 when compared with C1.

Expression of Fas Antigen in the Normal Mouse Brain

1998 ◽  
Vol 252 (3) ◽  
pp. 623-628 ◽  
Author(s):  
Cheho Park ◽  
Kazuhiro Sakamaki ◽  
Osamu Tachibana ◽  
Tetsumori Yamashima ◽  
Junkoh Yamashita ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Normal Mouse ◽  
Fas Antigen ◽  
Normal Mouse Brain

A morphological and ultrastructural investigation of normal mouse brain tissue after intracerebral injection of tumor necrosis factor

1992 ◽  
Vol 77 (2) ◽  
pp. 279-287 ◽  
Author(s):  
Toshiki Yamasaki ◽  
Haruhiko Kikuchi ◽  
Kouzo Moritake ◽  
Seiichi Nagao ◽  
Kouichi Iwasaki ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Brain Tissue ◽  
Mouse Brain ◽  
Tumor Necrosis ◽  
Normal Mouse ◽  
Mononuclear Cell Infiltration ◽  
Content Type ◽  
Normal Mouse Brain ◽  
Necrosis Factor ◽  
Fine Print

✓ Morphological and ultrastructural changes in normal mouse brain tissue were investigated after intracerebral stereotactic injections of tumor necrosis factor (specific activity: 2.0 × 106 U/mg protein) into the right frontal lobe. The mice received either a single infusion or multiple tumor necrosis factor infusions in three different dose groups (10, 100, or 500 U). Compared with sham-treated control mice that received adjusted intracerebral injections of purified albumin, the tumor necrosis factor-treated mice in all dose groups did not show any specific in vivo behavioral abnormalities during the 2 months of study following the infusions. Histological studies revealed hemorrhage attributable to the mechanics of the intracerebral infusions, a thickening of the arachnoid membranes, a reactive gliosis, and neutrophilic and/or mononuclear cell infiltration along the infusion pathway. A local neutrophilic response was prominent 1 day after tumor necrosis factor injection. An immunohistochemical analysis indicated that the mononuclear cell infiltration consisted of lymphocytes and macrophages. Except for the transient neutrophilic infiltration, these histological alterations did not differ from those seen in the sham-treated control groups, and most nonspecific reactive changes disappeared within 8 weeks after the injections. Furthermore, an ultrastructural study showed no apparent pathological changes in the cytoplasmic organelles of neuronal, glial, and endothelial cells in the tumor necrosis factor-injected mouse specimens. These results suggest that the tumor necrosis factor injections caused no specific toxicity and did not alter the parenchymal and stromal cells comprising normal mouse brain tissue.

Phosphorylated α-synuclein in normal mouse brain

FEBS Letters ◽  
2004 ◽  
Vol 572 (1-3) ◽  
pp. 227-232 ◽  
Author(s):  
Yu Hirai ◽  
Shinobu C Fujita ◽  
Takeshi Iwatsubo ◽  
Masato Hasegawa
Keyword(s):  
Mouse Brain ◽  
Normal Mouse ◽  
Normal Mouse Brain

Transplanted Transgenically Marked Oligodendrocytes Survive, Migrate and Myelinate in the Normal Mouse Brain as They Do in the Shiverer Mouse Brain

1994 ◽  
Vol 6 (5) ◽  
pp. 814-824 ◽  
Author(s):  
F. Lachapelle ◽  
E. Duhamel-Clerin ◽  
A. Gansmüller ◽  
A. Baron-Van Evercooren ◽  
H. Villarroya ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Normal Mouse ◽  
Normal Mouse Brain

Apolipoprotein E is found in astrocytes but not in microglia in the normal mouse brain

1999 ◽  
Vol 35 (2) ◽  
pp. 123-133 ◽  
Author(s):  
Shinobu C Fujita ◽  
Keiko Sakuta ◽  
Reiko Tsuchiya ◽  
Hiroki Hamanaka
Keyword(s):  
Apolipoprotein E ◽  
Mouse Brain ◽  
Normal Mouse ◽  
Normal Mouse Brain
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