scholarly journals A modular and divergent approach to spirocyclic pyrrolidines

2020 ◽  
Vol 11 (38) ◽  
pp. 10354-10360 ◽  
Author(s):  
Benjamin D. A. Shennan ◽  
Peter W. Smith ◽  
Yusuke Ogura ◽  
Darren J. Dixon
Keyword(s):  
Divergent Sequence

A three-step, modular and divergent sequence accessing challenging spirocyclic pyrrolidines has been developed, featuring a novel reductive spirocyclization cascade.

scholarly journals Activation of the lac Repressor in the Transgenic Mouse

Genetics ◽  
1997 ◽  
Vol 147 (1) ◽  
pp. 297-304
Author(s):  
Heidi Scrable ◽  
Peter J Stambrook
Keyword(s):  
Copy Number ◽  
Methylation Status ◽  
Lac Repressor ◽  
The Other ◽  
Lac Operon ◽  
Normal Process ◽  
Divergent Sequence ◽  
Somatic Tissues ◽  
New Material ◽  
Bacterial Sequence

Abstract We have introduced sequences encoding the lac repressor of Escherichia coli into the genome of the mouse. One sequence was derived from the bacterial lac operon and the other was created by reencoding the amino acid sequence of lacI with mammalian codons. Both versions are driven by an identical promoter fragment derived from the human β-actin locus and were microinjected into genetically identical pronuclear stage embryos. All transgenes utilizing the bacterial coding sequence were transcriptionally silent in all somatic tissues tested. The sequence re-encoded with mammalian codons was transcriptionally active at all transgene loci and expressed ubiquitously. Using methylation-sensitive enzymes, we have determined the methylation status of lac repressor transgenes encoded by either the bacterial or mammalian sequence. The highly divergent bacterial sequence was hypermethylated at all transgene loci, while the mammalian sequence was only hypermethylated at a high copy number locus. This may reflect a normal process that protects the genome from acquiring new material that has an abnormally divergent sequence or structure.

scholarly journals A One-Pot Divergent Sequence to Pyrazole and Quinoline Derivatives

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2160
Author(s):  
Guido Gambacorta ◽  
David C. Apperley ◽  
Ian R. Baxendale
Keyword(s):  
Scientific Literature ◽  
Building Blocks ◽  
Quinoline Derivatives ◽  
Divergent Sequence ◽  
One Pot

The hydroxy-pyrazole and 3-hydroxy-oxindole motifs have been utilised in several pharma and agrochemical leads but are distinctly underrepresented in the scientific literature due to the limited routes of preparation. We have developed a one-pot procedure for their synthesis starting from simple isatins. The method employs cheap and easy-to-handle building blocks and allows easy isolation.

scholarly journals Distribution of the Mosaic StructuredmurM Genes among Natural Populations ofStreptococcus pneumoniae

2000 ◽  
Vol 182 (23) ◽  
pp. 6798-6805 ◽  
Author(s):  
Sergio R. Filipe ◽  
Elena Severina ◽  
Alexander Tomasz
Keyword(s):  
Sequence Variation ◽  
Natural Populations ◽  
Laboratory Strain ◽  
Great Majority ◽  
Large Collection ◽  
Divergent Sequence ◽  
Chromatography Analysis ◽  
Exact Position ◽  
Complete Sequencing ◽  
Resistant Strains

ABSTRACT The presence and sequence variation of the murM gene were studied in a large collection (814 strains) of genetically diverseStreptococcus pneumoniae isolates, which included 27 different serogroups and both penicillin-resistant (423 isolates, 67 pulsed-field gel electrophoretic [PFGE] types) and intermediately penicillin-resistant (165 isolates, 66 PFGE types) and penicillin-susceptible (226 isolates, 135 PFGE types) strains. Diversity of the murM sequences was tested by hybridization with mainly two kinds of probes: one derived from the amplification of the nucleotide sequence between nucleotides 201 and 624 in the penicillin-susceptible laboratory strain R36A (murMA probe) and a second probe that amplified the comparable, highly divergent sequence in the penicillin-resistant strain Pen6 (murMBprobe). The great majority of the strains (761 of 814), including both penicillin-susceptible and penicillin-resistant isolates, reacted exclusively with the murMA probe. A smaller group of penicillin-resistant strains (48 of 814 isolates) reacted only with themurMB DNA probe, and an additional 5 isolates reacted with both probes. High-pressure liquid chromatography analysis of the peptidoglycan of strains hybridizing with murMB showed that they invariably contained an increased proportion of branched peptides. Complete sequencing of murM from a group of penicillin-resistant isolates allowed the identification of a number of different murMB alleles that differed in the length and exact position of the divergent (Pen6 type) sequences within the particular murM. The close similarity of these divergent sequences in the various murM alleles suggests a possible common heterologous origin.

scholarly journals A single transcription factor binds to two divergent sequence elements with a common function in cardiac myosin light chain-2 promoter.

1992 ◽  
Vol 12 (3) ◽  
pp. 1107-1116 ◽  
Author(s):  
P Qasba ◽  
E Lin ◽  
M D Zhou ◽  
A Kumar ◽  
M A Siddiqui
Keyword(s):  
Transcription Factor ◽  
Gene Transcription ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Cardiac Myosin ◽  
Divergent Sequence ◽  
Specific Element ◽  
Myosin Light Chain 2 ◽  
Sequence Elements ◽  
Gel Shift

The cardiac myosin light chain-2 (MLC-2) gene promoter contains several positive and negative cis-acting sequences that are involved in the regulation of its expression. We describe here the properties of two activator sequences, elements A and P, and their DNA-binding factors (ABFs). Element A (CCAAAAGTGG), located at -61, has homology with the evolutionarily conserved sequence CC(A/T)6GG, present in the genes of many contractile proteins. Element P (TAACCTTGAAAGC), located 114 bp upstream of element A, is conserved in both chicken and rat cardiac MLC-2 gene promoters. Deletion mutagenesis demonstrated that these two elements are involved in the positive regulation of MLC-2 gene transcription. At least two sequence-specific element A-binding proteins, ABF-1 and ABF-2, were identified by gel shift analysis of the fractionated cardiac nuclear proteins. ABF-1 binds to element A with strict dependence on the internal element A sequence AAAAGT. In contrast, ABF-2 exhibits a relaxed sequence requirement, as it recognizes the consensus CArG and CCAAT box sequences as well. ABF-2 also recognizes the distal element P despite the fact that the sequences of elements A and P are divergent. DNase I footprinting, methylation interference, and gel shift analyses demonstrated unequivocally that the element A-DNA affinity-purified protein ABF-2 binds to element P with sequence specificity. Since both elements A and P play a positive regulatory role in MLC-2 gene transcription and bind to a single protein (ABF-2), it would appear that ABF-2 is a key transcription factor with the ability to recognize divergent sequence elements involved in a common regulatory pathway during myogenesis.

A Divergent Sequence of Romberg Integrals

2019 ◽  
Vol 75 (1) ◽  
Author(s):  
André Pierro de Camargo
Keyword(s):  
Divergent Sequence

scholarly journals Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl-CoA:acyl carrier protein transacylase domains in modular polyketide synthases

FEBS Letters ◽  
1995 ◽  
Vol 374 (2) ◽  
pp. 246-248 ◽  
Author(s):  
Stephen F. Haydock ◽  
Jesús F. Aparicio ◽  
István Molnár ◽  
Torsten Schwecke ◽  
Lake Ee Khaw ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Polyketide Synthases ◽  
Carrier Protein ◽  
Sequence Motifs ◽  
Divergent Sequence

scholarly journals RH-regular transformations which sums a given double sequence

Filomat ◽  
2013 ◽  
Vol 27 (4) ◽  
pp. 625-627 ◽  
Author(s):  
Richard Patterson
Keyword(s):  
Double Sequence ◽  
Divergent Sequence ◽  
Multidimensional Analog

In 1946 P. Erdos and P. C Rosenbloom presented the following theorem that arose out of discussions they had with R. P. Agnew. Let {xn} be a bounded divergent sequence. Suppose that {xn} is summable by every regular Toeplitz method which sums {xn}. Then {yn} is of the form {cxn + an} where {an} is convergent. The goals of the paper includes the presentation of a multidimensional analog of Erdos and Rosenbloom results in [1].

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