scholarly journals Cytosine-5 methylation-directed construction of a Au nanoparticle-based nanosensor for simultaneous detection of multiple DNA methyltransferases at the single-molecule level

2020 ◽  
Vol 11 (35) ◽  
pp. 9675-9684
Author(s):  
Li-Juan Wang ◽  
Xiao Han ◽  
Jian-Ge Qiu ◽  
BingHua Jiang ◽  
Chun-Yang Zhang
Keyword(s):  
Single Molecule ◽  
Simultaneous Detection ◽  
Dna Methyltransferases ◽  
Sensitive Detection ◽  
Au Nanoparticle ◽  
Single Molecule Level

Cytosine-5 methylation-directed construction of Au nanoparticle-based nanosensors enables specific and sensitive detection of multiple DNA methyltransferases.

scholarly journals Simultaneous sensitive detection of multiple DNA glycosylases from lung cancer cells at the single-molecule level

2018 ◽  
Vol 9 (3) ◽  
pp. 712-720 ◽  
Author(s):  
Juan Hu ◽  
Ming-hao Liu ◽  
Ying Li ◽  
Bo Tang ◽  
Chun-yang Zhang
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Single Molecule ◽  
Simultaneous Detection ◽  
Sensitive Detection ◽  
Lung Cancer Cells ◽  
Dna Glycosylase ◽  
Dna Glycosylases ◽  
Single Molecule Level ◽  
Alkyladenine Dna Glycosylase

We demonstrate the simultaneous detection of human 8-oxoguanine DNA glycosylase 1 and human alkyladenine DNA glycosylase at the single-molecule level.

scholarly journals Rolling circle amplification-driven encoding of different fluorescent molecules for simultaneous detection of multiple DNA repair enzymes at the single-molecule level

2020 ◽  
Vol 11 (22) ◽  
pp. 5724-5734
Author(s):  
Chen-chen Li ◽  
Hui-yan Chen ◽  
Juan Hu ◽  
Chun-yang Zhang
Keyword(s):  
Dna Repair ◽  
Single Molecule ◽  
Rolling Circle Amplification ◽  
Simultaneous Detection ◽  
Single Molecule Detection ◽  
Dna Repair Enzymes ◽  
Rolling Circle ◽  
Single Molecule Level ◽  
Repair Enzymes ◽  
Fluorescent Molecules

Integration of single-molecule detection with rolling circle amplification-driven encoding of different fluorescent molecules enables simultaneous detection of multiple DNA repair enzymes.

scholarly journals Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at single-molecule level

2021 ◽  
Author(s):  
Chen-Chen Li ◽  
Hui-yan Chen ◽  
Xiliang Luo ◽  
Juan Hu ◽  
Chun-yang Zhang
Keyword(s):  
Lung Cancer ◽  
Clinical Diagnosis ◽  
Single Molecule ◽  
Simultaneous Measurement ◽  
Signal Amplification ◽  
Simultaneous Detection ◽  
Single Molecule Detection ◽  
Single Molecule Level ◽  
Multicolor Fluorescence ◽  
Cancer Tissues

The simultaneous detection of multiple microRNAs (miRNAs) will facilitate the early clinical diagnosis. Herein, we demonstrate the integration of multicolor fluorophores-encoded cascade signal amplification with single-molecule detection for simultaneous measurement...

Atomic Force Microscopy (AFM) for Topography and Recognition Imaging at Single Molecule Level

2013 ◽  
pp. 102-112
Author(s):  
Memed Duman ◽  
Andreas Ebner ◽  
Christian Rankl ◽  
Jilin Tang ◽  
Lilia A. Chtcheglova ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Single Molecule Level ◽  
Force Microscopy ◽  
Atomic Force ◽  
Recognition Imaging

Curaxin-Induced DNA Topology Alterations Trigger the Distinct Binding Response of CTCF and FACT at the Single-Molecule Level

Biochemistry ◽  
2021 ◽  
Vol 60 (7) ◽  
pp. 494-499
Author(s):  
Ke Lu ◽  
Cuifang Liu ◽  
Yinuo Liu ◽  
Anfeng Luo ◽  
Jun Chen ◽  
...  
Keyword(s):  
Single Molecule ◽  
Dna Topology ◽  
Single Molecule Level

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

scholarly journals Power-law behavior of transcription factor dynamics at the single-molecule level implies a continuum affinity model

2021 ◽  
Author(s):  
David A Garcia ◽  
Gregory Fettweis ◽  
Diego M Presman ◽  
Ville Paakinaho ◽  
Christopher Jarzynski ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Single Molecule ◽  
Power Law ◽  
Dwell Time ◽  
Specific Binding ◽  
Power Law Distribution ◽  
Single Molecule Level ◽  
Binding Behavior ◽  
Chromatin Template ◽  
Nuclear Domains

Abstract Single-molecule tracking (SMT) allows the study of transcription factor (TF) dynamics in the nucleus, giving important information regarding the diffusion and binding behavior of these proteins in the nuclear environment. Dwell time distributions obtained by SMT for most TFs appear to follow bi-exponential behavior. This has been ascribed to two discrete populations of TFs—one non-specifically bound to chromatin and another specifically bound to target sites, as implied by decades of biochemical studies. However, emerging studies suggest alternate models for dwell-time distributions, indicating the existence of more than two populations of TFs (multi-exponential distribution), or even the absence of discrete states altogether (power-law distribution). Here, we present an analytical pipeline to evaluate which model best explains SMT data. We find that a broad spectrum of TFs (including glucocorticoid receptor, oestrogen receptor, FOXA1, CTCF) follow a power-law distribution of dwell-times, blurring the temporal line between non-specific and specific binding, suggesting that productive binding may involve longer binding events than previously believed. From these observations, we propose a continuum of affinities model to explain TF dynamics, that is consistent with complex interactions of TFs with multiple nuclear domains as well as binding and searching on the chromatin template.

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