scholarly journals A one step method for isolation of genomic DNA using multi-amino modified magnetic nanoparticles

RSC Advances ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 3324-3332
Author(s):  
Jia Xu ◽  
Dan Chen ◽  
Yuan Yang ◽  
Hongjian Gong ◽  
Wenqi Gao ◽  
...  

Schematic procedure of DNA isolation using mAMNPs.

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Ying Li ◽  
Ning Tang ◽  
Fuyuhiko Inagaki ◽  
Chisato Mukai ◽  
Kazuichi Hayakawa

1,3-Dialkylimidazolium-based ionic liquids were chemically synthesized and bonded on the surface of magnetic nanoparticles (MNPs) with easy one-step reaction. The obtained six kinds of ionic liquid modified MNPs were characterized with transmission electron microscopy, thermogravimetric analysis, magnetization, and FTIR, which owned the high adsorption capacity due to the nanometer size and high-density modification with ionic liquids. Functionality of MNPs with ionic liquids greatly influenced the solubility of the MNPs with organic solvents depending on the alkyl chain length and the anions of the ionic liquids. Moreover, the obtained MNPs showed the specific extraction efficiency to organic pollutant, polycyclic aromatic hydrocarbons, while superparamagnetic property of the MNPs facilitated the convenient separation of MNPs from the bulks water samples.


2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Yolanda Osuna ◽  
Karla M. Gregorio-Jauregui ◽  
J. Gerardo Gaona-Lozano ◽  
Iliana M. de la Garza-Rodríguez ◽  
Anna Ilyna ◽  
...  

Chitosan-coated magnetic nanoparticles (CMNP) were obtained at 50°C in a one-step method comprising coprecipitation in the presence of low chitosan content. CMNP showed high magnetization and superparamagnetism. They were composed of a core of 9.5 nm in average diameter and a very thin chitosan layer in accordance with electron microscopy measurements. The results from Fourier transform infrared spectrometry demonstrated that CMNP were obtained and those from thermogravimetric analysis allowed to determine that they were composed of 95 wt% of magnetic nanoparticles and 5 wt% of chitosan. 67% efficacy in the Pb+2removal test indicated that only 60% of amino groups on CMNP surface bound to Pb, probably due to some degree of nanoparticle flocculation during the redispersion. The very low weight ratio chitosan to magnetic nanoparticles obtained in this study, 0.053, and the high yield of the precipitation reactions (≈97%) are noticeable.


Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Arianna Marengo ◽  
Cecilia Cagliero ◽  
Barbara Sgorbini ◽  
Jared L. Anderson ◽  
Miranda N. Emaus ◽  
...  

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Karla M. Gregorio-Jauregui ◽  
Ma. Guadalupe Pineda ◽  
Jorge E. Rivera-Salinas ◽  
Gil Hurtado ◽  
Hened Saade ◽  
...  

Preparation of magnetic nanoparticles coated with chitosan in one step by the coprecipitation method in the presence of different chitosan concentrations is reported here. Obtaining of magnetic superparamagnetic nanoparticles was confirmed by X-ray diffraction and magnetic measurements. Scanning transmission electron microscopy allowed to identify spheroidal nanoparticles with around 10-11 nm in average diameter. Characterization of the products by Fourier transform infrared spectroscopy demonstrated that composite chitosan-magnetic nanoparticles were obtained. Chitosan content in obtained nanocomposites was estimated by thermogravimetric analysis. The nanocomposites were tested in Pb2+removal from a PbCl2aqueous solution, showing a removal efficacy up to 53.6%. This work provides a simple method for chitosan-coated nanoparticles obtaining, which could be useful for heavy metal ions removal from water.


1993 ◽  
Vol 58 (11) ◽  
pp. 2642-2650 ◽  
Author(s):  
Zdeněk Kruliš ◽  
Ivan Fortelný ◽  
Josef Kovář

The effect of dynamic curing of PP/EPDM blends with sulfur and thiuram disulfide systems on their mechanical properties was studied. The results were interpreted using the knowledge of the formation of phase structure in the blends during their melt mixing. It was shown, that a sufficiently slow curing reaction is necessary if a high impact strength is to be obtained. Only in such case, a fine and homogeneous dispersion of elastomer can be formed, which is the necessary condition for high impact strength of the blend. Using an inhibitor of curing in the system and a one-step method of dynamic curing leads to an increase in impact strength of blends. From the comparison of shear modulus and impact strength values, it follows that, at the stiffness, the dynamically cured blends have higher impact strength than the uncured ones.


2019 ◽  
Vol 375 ◽  
pp. 122000 ◽  
Author(s):  
Yang Xuan ◽  
Xian-Lin Song ◽  
Xiao-Quan Yang ◽  
Ruo-Yun Zhang ◽  
Zi-Yu Song ◽  
...  

2020 ◽  
Vol 16 (1) ◽  
pp. 1-10
Author(s):  
Tomáš Solný ◽  
Petr Ptáček ◽  
Tomáš Opravil ◽  
Lucie Dlabajová ◽  
Matěj Březina ◽  
...  

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