scholarly journals Investigation of the detoxification effect of licorice on Semen Strychni-induced acute toxicity in rats using a HPLC-Q-TOF/MS-based metabolomics approach

RSC Advances ◽  
2020 ◽  
Vol 10 (72) ◽  
pp. 44398-44407
Author(s):  
Yalan Xiang ◽  
Chao Wang ◽  
Jing Wen ◽  
Min Zhang ◽  
Xiaoyu Duan ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Semen Strychni ◽  
Tof Ms

Biomarkers and metabolomic pathway provide an integral understanding for the acute toxicity of Semen Strychni and the detoxification effect of licorice.

scholarly journals The Correlation between Chemical Composition, as Determined by UPLC-TOF-MS, and Acute Toxicity ofVeratrum nigrumL. andRadix paeoniae alba

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Xianxie Zhang ◽  
Yuguang Wang ◽  
Qiande Liang ◽  
Zengchun Ma ◽  
Chengrong Xiao ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Acute Toxicity ◽  
Chemical Constituents ◽  
Two Agents ◽  
Veratrum Alkaloids ◽  
Radix Paeoniae Alba ◽  
Insight Into ◽  
Tof Ms

The eighteen incompatible medicaments is an important theory in traditional Chinese medicine. The theory suggests that drugs in the eighteen incompatible medicaments can be toxic when used together.Veratrum nigrumL. andRadix paeoniae albabelong to the eighteen incompatible medicaments and have been prohibited for thousands of years. This study offers preliminary insight into the mechanism and chemical constituents responsible for the incompatibility and toxicity of these two agents. Specifically, we performed toxicology studies to identify and quantify the constituent substances of the two agents. Experiments revealed that acute toxicity increases when the dose ofV. nigrumL. is higher than, or equal to, RPA. UPLC-TOF-MS analysis showed that, although the volumes ofV. nigrumL. were the same, the content of some veratrum alkaloids changed significantly and had a trend toward a highly positive correlationr≥0.8with toxicity. This suggests that the increased toxicity of theV. nigrumL. and RPA combination was due mainly to increased content of the special veratrum alkaloids. The cytotoxicity of veratridine in SH-SY5Y cells was decreased with increasing paeoniflorin concentrations. This study provides insight into the mechanism behind the incompatibility theory of TCM.

scholarly journals Pharmacokinetic, acute toxicity, and pharmacodynamic studies of semen strychni total alkaloid microcapsules

2021 ◽  
Vol 18 (9) ◽  
pp. 1985-1992
Author(s):  
Xinli Song ◽  
Daobin Yang ◽  
Yunxia Wang ◽  
Wen Liu ◽  
Yonglin Wang ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Half Life ◽  
Total Alkaloid ◽  
Pain Threshold ◽  
Low Dose ◽  
High Dose ◽  
Total Alkaloids ◽  
Liquid Chromatography Tandem Mass ◽  
Pain Inhibition ◽  
Semen Strychni

Purpose: To investigate the safety and effectiveness of semen strychni total alkaloid microcapsules (SSTAM), compared with semen strychni total alkaloids (SSTA). Methods: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed to assess pharmacokinetics of brucine and strychnine in rats. Acute toxicity was investigated in pre-test and formal experiments in mice. The pharmacodynamics of SSTAM and SSTA were evaluated by their analgesic and anti-inflammatory activities. Results: With respect to brucine, the half-life of SSTA group (1.6 mg/kg), low-dose SSTAM group (6 mg/kg) and high-dose SSTAM group (10 mg/kg) was 5.723, 9.321 and 9.025 h, respectively. With respect to strychnine, the half-life of SSTA group, low-dose SSTAM group and high-dose SSTAM group was 4.065, 8.819 and 8.654 h, respectively. The LD50 values of SSTAM group and SSTA group were 236.59 and 30.27 mg/kg, respectively. The pain inhibition rates of SSTAM groups (25 and 50 mg/kg) were higher than that of SSTA group (p < 0.05) while the pain threshold values of the SSTAM groups (25 and 50 mg/kg) were higher than that of blank control (p < 0.01) and SSTA groups (p < 0.01) at 60 min and 120 min. The inhibition rates of the SSTAM groups (25 and 50 mg/kg) were higher than that of SSTA group based on ear swelling and cotton ball granulation tests. Compared with blank control and SSTA groups, the absorbance values of SSTAM groups (25 and 50 mg/kg) were lower (p < 0.01). Conclusion: SSTAM increases the dosage of administration but reducea the toxicity of the alkaloids in rats, and is thus a potentially safe and effective drug delivery system.

Determination and Toxicology Studies of Quaternary Ammonium Salts in Solution after Organoclay Processing

2018 ◽  
Vol 762 ◽  
pp. 368-372
Author(s):  
Matiss Pals ◽  
Ieva Putna-Nimane ◽  
Juris Kostjukovs ◽  
Jūlija Karasa ◽  
Solvita Kostjukova ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Daphnia Magna ◽  
Quaternary Ammonium ◽  
Toxicity Test ◽  
Quaternary Ammonium Salts ◽  
Ammonium Salts ◽  
Acute Toxicity Test ◽  
Innovative Materials ◽  
Tof Ms

Organoclays have potential to be used as additives in various facade paints and other finishing materials. Such new innovative materials must be rigorously tested for them to have no impact on environment. Focus of this study was to develop HPLC – TOF/MS method for determining quaternary ammonium salts in various material washing solutions and to determine organoclay toxicity by performing acute toxicity test on Daphnia magna. From the obtained results, it was possible to conclude that organoclays can be safely used as additives in various materials with low or no impact on environment. We determined that organoclays are significantly less toxic then some quaternary ammonium salts on its own.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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